925 research outputs found
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly
Determining the ages of sub-fossil cetacean remains, found in the Carse of Stirling, Scotland
During the 19th and early 20th centuries, sub-fossil cetacean remains were often discovered in the Firth of Forth, Central Scotland. These bones and skeletons of "Whales" were excavated from a recent, estuarine deposit (named "carse clay") and, within the biological and geological sciences, were not judged to be important. That palaeontological evidence is re-evaluated in this thesis. These cetacean remains have been preserved in an unusual marine environment and form an exceptional fossil assemblage, with almost no geological precedents. Why is it there?
Whatever caused exceptional preservation in the Firth of Forth in the early Holocene (c. 9.5 – 2.5ka cal BP) can be best identified with chronological data. The ages of six sets of cetacean remains are determined in this thesis, by radiocarbon dating and stratigraphic inference. To reconstruct where a bone or skeleton had been found in the "carse" and then to identify any surviving elements in modern museum collections, archaic textual sources had to be thoroughly investigated. Radiocarbon dates from marine organisms require correction for "reservoir effects" and those applicable to mysticete cetaceans require careful consideration.
The absolute dating evidence shows that no two "Whales" are the same age and that each died, and was then preserved, over the period 9.5 – 7.0ka cal BP. Therefore, a "disaster" (e.g. tsunami) or mass mortality is unlikely to have caused these remains to accumulate. A combination of physical processes and stable environmental conditions are more likely responsible, and might still permit exceptional preservation in the modern Firth of Forth. Actualistic experiment (observing if, and how, a cetacean carcass is preserved or dispersed on a modern tidal foreshore) would allow further insights into this cryptic palaeontological assemblage
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Methane Production by Methanogens In Simulated Subsurface Martian Environments
Methane has a typical atmospheric photochemical lifetime of ∼300 years on Mars, making contemporary reported detections (and non-detections) of methane a fiercely debated topic, due to the potential need for a present-day source. On Earth, most methane is produced by methanogenic microbes present in, e.g., ruminants, wetlands, lakes and permafrost. Of the four metabolic pathways on Earth, the hydrogenotrophic pathway is the most common, utilising CO2 and H2 as substrates. Both gases are present on Mars, plus liquid water and essential elements (CHNOPS) that are requirements for life, and organics. Surface conditions on Mars are sterilising, however, the temperature and pressure of the subsurface are potentially favourable to life and provide a shield to sterilising surface conditions, and are thus a possible habitat for methanogens. A meta-analysis was
conducted, motivated by these subsurface parameters, that redefined the statistical representation for several growth parameters for all type-strains of methanogens and analysed multiple parameters
simultaneously across multiple categories (e.g. metabolism), showing that the optimal average conditions in which to grow methanogens would be a meso-temperate (20 to 39◦C), hypersaline and slightly acidic environment. Two martian subsurface environments were simulated to determine whether environmental or chemical factors are inhibitory to methanogenesis. (1) Methanoculleus marisnigri was grown in a custom-built, high-pressure manifold at 60 bar and 25◦C to simulate the subsurface of Mars, although no methane was produced, due to a technical issue resulting in oxygenated medium. However, some cells survived five weeks of oxygenation. (2) Methanothermococcus okinawensis was grown in a simulated chemical environment at 1 bar and 60◦C, that included a regolith simulant, a proposed martian brine and a Mars-relevant organic source (carbonaceous chondrite). The simulated parameters of the chemical environment of subsurface Mars were not inhibitory to hydrogenotrophic methanogenesis, suggesting it is feasible (from a metabolic perspective) that subsurface methanogens could be producing contemporary methane on Mars
Relatively Absolute : Relative and Absolute Chronologies in the Neolithic of Southeast Europe
Зборник радова на тему апсолутне и релативне хронологије неолитског периода у југоисточној Европи. Географски покрива области од Грчке до Хрватске, а хронолошки период између 7000 и 4500 године пре нове ере. У зборнику су приказани најновији приступи и резултати радиокарбонских анализа и статистички и типолошки модели који побољшавају прецизност резултата
Geoarchaeological Investigations of Late Pleistocene Physical Environments and Impacts of Prehistoric Foragers on the Ecosystem in Northern Malawi and Austria
A growing body of research shows that not only did environmental changes play an important role in human evolution, but humans in turn have impacted ecosystems and landscape evolution since the Late Pleistocene. This thesis presents collaborative work on Late Pleistocene open-air sites in the Karonga District of northern Malawi, in which new aspects of forager behavior came to light through the reconstruction of physical environments. My work has helped recognize that late Middle Stone Age (MSA) activity and tool production occurred in locally more open riparian environments within evergreen gallery forest, surrounded by a regional vegetation dominated by miombo woodlands and savanna. Additionally, MSA hunter-gatherers exploited the confluence of river and wetland areas along the shores of Lake Malawi, which likely served as important corridors for the dispersal of biota. By comparing data from the archaeological investigations with lake core records, we were able to identify effects of anthropogenic burning on vegetation structures and sedimentation in the region as early as 80 thousand years ago. These findings not only proved it possible to uncover early impacts of human activity on the ecosystem, but also emphasize the importance of fire in the lives of early foragers.
Publications contained within this dissertation:
A. Wright, D.K., Thompson, J.C., Schilt, F.C., Cohen, A., Choi, J-H., Mercader, J., Nightingale, S., Miller, C.E., Mentzer, S.M., Walde, D., Welling, M., and Gomani-Chindebvu, E. “Approaches to Middle Stone Age landscape archaeology in tropical Africa”. Special issue Geoarchaeology of the Tropics of Journal of Archaeological Science 77:64-77. http://dx.doi.org/10.1016/j.jas.2016.01.014
B. Schilt, F.C., Verpoorte, A., Antl, W. “Micromorphology of an Upper Paleolithic cultural layer at Grub-Kranawetberg, Austria”. Journal of Archaeological Science: Reports 14:152-162. http://dx.doi.org/10.1016/j.jasrep.2017.05.041
C. Nightingale, S., Schilt, F.C., Thompson, J.C., Wright, D.K., Forman, S., Mercader, J., Moss, P., Clarke, S. Itambu, M., Gomani-Chindebvu, E., Welling, M. Late Middle Stone Age Behavior and Environments at Chaminade I (Karonga, Malawi). Journal of Paleolithic Archaeology 2-3:258-397. https://doi.org/10.1007/s41982-019-00035-3
D. Thompson, J.C.*, Wright, D.K.*, Ivory, S.J.*, Choi, J-H., Nightingale, S., Mackay, A., Schilt, F.C., Otárola-Castillo, E., Mercader, J., Forman, S.L., Pietsch, T., Cohen, A.S., Arrowsmith, J.R., Welling, M., Davis, J., Schiery, B., Kaliba, P., Malijani, O., Blome, M.W., O’Driscoll, C., Mentzer, S.M., Miller, C., Heo, S., Choi, J., Tembo, J., Mapemba, F., Simengwa, D., and Gomani-Chindebvu, E. “Early human impacts and ecosystem reorganization in southern-central Africa”. Science Advances 7(19): eabf9776. *equal contribution https://doi.org/10.1126/sciadv.abf9776
E. Schilt, F.C., Miller, C.M., Wright, D.K., Mentzer, S.M., Mercader, J., Moss, Choi, J.-H., Siljedal, G., Clarke, S., Mwambwiga, A., Thomas, K., Barbieri, A., Kaliba, P., Gomani-Chindebvu, E., Thompson, J.C. “Hunter-gatherer environments at the Late Pleistocene sites of Bruce and Mwanganda´s Village, northern Malawi”. Quaternary Science Reviews 292: 107638. https://www.sciencedirect.com/science/article/pii/S0277379122002694 [untranslated
First peopling of the Americas : modelling the palaeo-landscape and potential Upper Palaeolithic human migration routes
Le peuplement des Amériques fut le dernier grand événement migratoire de Homo sapiens et nous méconnaissons toujours les détails à son sujet. Des débats surgissent concernant l’environnement, les populations concernées, ainsi que les cultures impliquées. Malheureusement, des biais scientifiques persistent quant à la chronologie de cet événement et il peut donc être difficile de proposer quelque chose de nouveau. Avec ArcMap 10.7.1, nous présentons de nouveaux modèles de migrations terrestres basés sur les sentiers de moindre effort, retraçant les routes potentielles que les humains ont pu utiliser afin d’arriver en Amérique au cours du Pléistocène; nous surlignons les facteurs environnementaux, génétiques et archéologiques spécifiques qui doivent être considérés pour les modèles futurs, et nous présentons deux trajets de migration qui auraient pu avoir été utilisé pendant le Paléolithique Supérieur, élucidant par conséquent comment les humains sont arrivés pour la première fois dans le continent américain.The peopling of the Americas was the last great dispersal event of our species, Homo sapiens, and there is still so much we do not know about it. Debates arise concerning the environment, the populations involved, as well as the cultural or physical markers they might have left behind. Unfortunately, the debate concerning the First Peopling of North America is marked by scientific biases and it can thus be difficult to propose something new. Through ArcMap 10.7.1, we present a Least Cost model of terrestrial migrations from Asia to America, we highlight the specific environmental, genetic and archaeological factors that need to be considered in future models, and present two migration paths that could have been used during the Late Pleistocene, thus shedding light onto how humans first arrived in the American continent
BDS GNSS for Earth Observation
For millennia, human communities have wondered about the possibility of observing
phenomena in their surroundings, and in particular those affecting the Earth on which they live.
More generally, it can be conceptually defined as Earth observation (EO) and is the collection of
information about the biological, chemical and physical systems of planet Earth. It can be undertaken
through sensors in direct contact with the ground or airborne platforms (such as weather balloons and
stations) or remote-sensing technologies. However, the definition of EO has only become significant
in the last 50 years, since it has been possible to send artificial satellites out of Earth’s orbit.
Referring strictly to civil applications, satellites of this type were initially designed to provide
satellite images; later, their purpose expanded to include the study of information on land
characteristics, growing vegetation, crops, and environmental pollution. The data collected are used
for several purposes, including the identification of natural resources and the production of accurate
cartography. Satellite observations can cover the land, the atmosphere, and the oceans.
Remote-sensing satellites may be equipped with passive instrumentation such as infrared or
cameras for imaging the visible or active instrumentation such as radar. Generally, such satellites are
non-geostationary satellites, i.e., they move at a certain speed along orbits inclined with respect to the
Earth’s equatorial plane, often in polar orbit, at low or medium altitude, Low Earth Orbit (LEO) and
Medium Earth Orbit (MEO), thus covering the entire Earth’s surface in a certain scan time (properly
called ’temporal resolution’), i.e., in a certain number of orbits around the Earth.
The first remote-sensing satellites were the American NASA/USGS Landsat Program;
subsequently, the European: ENVISAT (ENVironmental SATellite), ERS (European Remote-Sensing
satellite), RapidEye, the French SPOT (Satellite Pour l’Observation de laTerre), and the Canadian
RADARSAT satellites were launched. The IKONOS, QuickBird, and GeoEye-1 satellites were
dedicated to cartography. The WorldView-1 and WorldView-2 satellites and the COSMO-SkyMed
system are more recent. The latest generation are the low payloads called Small Satellites, e.g., the
Chinese BuFeng-1 and Fengyun-3 series.
Also, Global Navigation Satellite Systems (GNSSs) have captured the attention of researchers
worldwide for a multitude of Earth monitoring and exploration applications. On the other hand,
over the past 40 years, GNSSs have become an essential part of many human activities. As is widely
noted, there are currently four fully operational GNSSs; two of these were developed for military
purposes (American NAVstar GPS and Russian GLONASS), whilst two others were developed for
civil purposes such as the Chinese BeiDou satellite navigation system (BDS) and the European
Galileo. In addition, many other regional GNSSs, such as the South Korean Regional Positioning
System (KPS), the Japanese quasi-zenital satellite system (QZSS), and the Indian Regional Navigation
Satellite System (IRNSS/NavIC), will become available in the next few years, which will have
enormous potential for scientific applications and geomatics professionals.
In addition to their traditional role of providing global positioning, navigation, and timing (PNT)
information, GNSS navigation signals are now being used in new and innovative ways. Across the
globe, new fields of scientific study are opening up to examine how signals can provide information
about the characteristics of the atmosphere and even the surfaces from which they are reflected before
being collected by a receiver.
EO researchers monitor global environmental systems using in situ and remote monitoring tools.
Their findings provide tools to support decision makers in various areas of interest, from security
to the natural environment. GNSS signals are considered an important new source of information
because they are a free, real-time, and globally available resource for the EO community
Computer Vision-Aided Intelligent Monitoring of Coffee: Towards Sustainable Coffee Production
Coffee which is prepared from the grinded roasted seeds of harvested coffee
cherries, is one of the most consumed beverage and traded commodity, globally.
To manually monitor the coffee field regularly, and inform about plant and soil
health, as well as estimate yield and harvesting time, is labor-intensive,
time-consuming and error-prone. Some recent studies have developed sensors for
estimating coffee yield at the time of harvest, however a more inclusive and
applicable technology to remotely monitor multiple parameters of the field and
estimate coffee yield and quality even at pre-harvest stage, was missing.
Following precision agriculture approach, we employed machine learning
algorithm YOLO, for image processing of coffee plant. In this study, the latest
version of the state-of-the-art algorithm YOLOv7 was trained with 324 annotated
images followed by its evaluation with 82 unannotated images as test data.
Next, as an innovative approach for annotating the training data, we trained
K-means models which led to machine-generated color classes of coffee fruit and
could thus characterize the informed objects in the image. Finally, we
attempted to develop an AI-based handy mobile application which would not only
efficiently predict harvest time, estimate coffee yield and quality, but also
inform about plant health. Resultantly, the developed model efficiently
analyzed the test data with a mean average precision of 0.89. Strikingly, our
innovative semi-supervised method with an mean average precision of 0.77 for
multi-class mode surpassed the supervised method with mean average precision of
only 0.60, leading to faster and more accurate annotation. The mobile
application we designed based on the developed code, was named CoffeApp, which
possesses multiple features of analyzing fruit from the image taken by phone
camera with in field and can thus track fruit ripening in real time
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Sonic heritage: listening to the past
History is so often told through objects, images and photographs, but the potential of sounds to reveal place and space is often neglected. Our research project ‘Sonic Palimpsest’1 explores the potential of sound to evoke impressions and new understandings of the past, to embrace the sonic as a tool to understand what was, in a way that can complement and add to our predominant visual understandings. Our work includes the expansion of the Oral History archives held at Chatham Dockyard to include women’s voices and experiences, and the creation of sonic works to engage the public with their heritage. Our research highlights the social and cultural value of oral history and field recordings in the transmission of knowledge to both researchers and the public. Together these recordings document how buildings and spaces within the dockyard were used and experienced by those who worked there. We can begin to understand the social and cultural roles of these buildings within the community, both past and present
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