1,296 research outputs found

    Unicast UDP Usage Guidelines for Application Designers

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    Full TCP/IP for 8-Bit architectures

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    We describe two small and portable TCP/IP implementations fulfilling the subset of RFC1122 requirements needed for full host-to-host interoperability. Our TCP/IP implementations do not sacrifice any of TCP's mechanisms such as urgent data or congestion control. They support IP fragment reassembly and the number of multiple simultaneous connections is limited only by the available RAM. Despite being small and simple, our implementations do not require their peers to have complex, full-size stacks, but can communicate with peers running a similarly light-weight stack. The code size is on the order of 10 kilobytes and RAM usage can be configured to be as low as a few hundred bytes

    The surface reactivity of iron oxide nanoparticles as a potential hazard for aquatic environments: A study on Daphnia magna adults and embryos

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    open5noNano-ecotoxicology is extensively debated and nanomaterial surface reactivity is an emerging topic. Iron oxide nanoparticles are widely applied, with organic or inorganic coatings for stabilizing their suspensions. Surface active maghemite nanoparticles (SAMNs) are the unique example of naked iron oxide displaying high colloidal and structural stability in water and chemical reactivity. The colloidal behavior of SAMNs was studied as a function of the medium salinity and protocols of acute and chronic toxicity on Daphnia magna were consequently adapted. SAMN distribution into the crustacean, intake/depletion rates and swimming performances were evaluated. No sign of toxicity was detected in two model organisms from the frst trophic level (P. subcapitata and L. minor). In D. magna, acute EC50 values of SAMN was assessed, while no sub-lethal efects were observed and the accumulation of SAMNs in the gut appeared as the sole cause of mortality. Fast depuration and absence of delayed efects indicated no retention of SAMNs within the organism. In spite of negligible toxicity on D. magna adults, SAMN surface reactivity was responsible of membrane bursting and lethality on embryos. The present study ofers a contribution to the nascent knowledge concerning the impact of nanoparticle surface reactivity on biological interfaces.openMassimiliano Magro, Marco De Liguoro, Eleonora Franzago, Davide Baratella, Fabio VianelloMagro, Massimiliano; DE LIGUORO, Marco; Franzago, Eleonora; Baratella, Davide; Vianello, Fabi

    A Backward/Forward Recovery Approach for the Preconditioned Conjugate Gradient Algorithm

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    Several recent papers have introduced a periodic verification mechanism to detect silent errors in iterative solvers. Chen [PPoPP'13, pp. 167--176] has shown how to combine such a verification mechanism (a stability test checking the orthogonality of two vectors and recomputing the residual) with checkpointing: the idea is to verify every dd iterations, and to checkpoint every c×dc \times d iterations. When a silent error is detected by the verification mechanism, one can rollback to and re-execute from the last checkpoint. In this paper, we also propose to combine checkpointing and verification, but we use algorithm-based fault tolerance (ABFT) rather than stability tests. ABFT can be used for error detection, but also for error detection and correction, allowing a forward recovery (and no rollback nor re-execution) when a single error is detected. We introduce an abstract performance model to compute the performance of all schemes, and we instantiate it using the preconditioned conjugate gradient algorithm. Finally, we validate our new approach through a set of simulations

    Accurate Prosthetic Hand

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    The purpose of this project is to explore a method to improve the dexterity of artificial hands by closely mimicking the biomechanics of a human hand. The mechanical system of the device is actuated using several stepper motors controlled by electroencephalogram (EEG) and electromyograph (EMG) signals. The majority of the device’s motions are controlled using EEG, with three distinct thoughts executing three distinct grips: pinch, hook, and point. EMG signals are used for finer motor control, such as controlling the strength of each grip pattern. The completion of this project resulted in a prosthetic hand prototype capable of nine degrees of freedom as well as the creation of a control system that relies on sensory input from the mind and body

    A next-generation sequencing method for overcoming the multiple gene copy problem in polyploid phylogenetics, applied to Poa grasses

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    <p>Abstract</p> <p>Background</p> <p>Polyploidy is important from a phylogenetic perspective because of its immense past impact on evolution and its potential future impact on diversification, survival and adaptation, especially in plants. Molecular population genetics studies of polyploid organisms have been difficult because of problems in sequencing multiple-copy nuclear genes using Sanger sequencing. This paper describes a method for sequencing a barcoded mixture of targeted gene regions using next-generation sequencing methods to overcome these problems.</p> <p>Results</p> <p>Using 64 3-bp barcodes, we successfully sequenced three chloroplast and two nuclear gene regions (each of which contained two gene copies with up to two alleles per individual) in a total of 60 individuals across 11 species of Australian <it>Poa </it>grasses. This method had high replicability, a low sequencing error rate (after appropriate quality control) and a low rate of missing data. Eighty-eight percent of the 320 gene/individual combinations produced sequence reads, and >80% of individuals produced sufficient reads to detect all four possible nuclear alleles of the homeologous nuclear loci with 95% probability.</p> <p>We applied this method to a group of sympatric Australian alpine <it>Poa </it>species, which we discovered to share an allopolyploid ancestor with a group of American <it>Poa </it>species. All markers revealed extensive allele sharing among the Australian species and so we recommend that the current taxonomy be re-examined. We also detected hypermutation in the <it>trn</it>H-<it>psb</it>A marker, suggesting it should not be used as a land plant barcode region. Some markers indicated differentiation between Tasmanian and mainland samples. Significant positive spatial genetic structure was detected at <100 km with chloroplast but not nuclear markers, which may be a result of restricted seed flow and long-distance pollen flow in this wind-pollinated group.</p> <p>Conclusions</p> <p>Our results demonstrate that 454 sequencing of barcoded amplicon mixtures can be used to reliably sample all alleles of homeologous loci in polyploid species and successfully investigate phylogenetic relationships among species, as well as to investigate phylogeographic hypotheses. This next-generation sequencing method is more affordable than and at least as reliable as bacterial cloning. It could be applied to any experiment involving sequencing of amplicon mixtures.</p
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