Use of multiple singular value decompositions to analyze complex intracellular calcium ion signals

We compare calcium ion signaling ($\mathrm {Ca}^{2+}$) between two exposures; the data are present as movies, or, more prosaically, time series of images. This paper describes novel uses of singular value decompositions (SVD) and weighted versions of them (WSVD) to extract the signals from such movies, in a way that is semi-automatic and tuned closely to the actual data and their many complexities. These complexities include the following. First, the images themselves are of no interest: all interest focuses on the behavior of individual cells across time, and thus, the cells need to be segmented in an automated manner. Second, the cells themselves have 100$+$ pixels, so that they form 100$+$ curves measured over time, so that data compression is required to extract the features of these curves. Third, some of the pixels in some of the cells are subject to image saturation due to bit depth limits, and this saturation needs to be accounted for if one is to normalize the images in a reasonably unbiased manner. Finally, the $\mathrm {Ca}^{2+}$ signals have oscillations or waves that vary with time and these signals need to be extracted. Thus, our aim is to show how to use multiple weighted and standard singular value decompositions to detect, extract and clarify the $\mathrm {Ca}^{2+}$ signals. Our signal extraction methods then lead to simple although finely focused statistical methods to compare $\mathrm {Ca}^{2+}$ signals across experimental conditions.Comment: Published in at http://dx.doi.org/10.1214/09-AOAS253 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

Dynamic Occupancy Grid Prediction for Urban Autonomous Driving: A Deep Learning Approach with Fully Automatic Labeling

Long-term situation prediction plays a crucial role in the development of intelligent vehicles. A major challenge still to overcome is the prediction of complex downtown scenarios with multiple road users, e.g., pedestrians, bikes, and motor vehicles, interacting with each other. This contribution tackles this challenge by combining a Bayesian filtering technique for environment representation, and machine learning as long-term predictor. More specifically, a dynamic occupancy grid map is utilized as input to a deep convolutional neural network. This yields the advantage of using spatially distributed velocity estimates from a single time step for prediction, rather than a raw data sequence, alleviating common problems dealing with input time series of multiple sensors. Furthermore, convolutional neural networks have the inherent characteristic of using context information, enabling the implicit modeling of road user interaction. Pixel-wise balancing is applied in the loss function counteracting the extreme imbalance between static and dynamic cells. One of the major advantages is the unsupervised learning character due to fully automatic label generation. The presented algorithm is trained and evaluated on multiple hours of recorded sensor data and compared to Monte-Carlo simulation