1,534 research outputs found
Sequence-structure relations of pseudoknot RNA
<p>Abstract</p> <p>Background</p> <p>The analysis of sequence-structure relations of RNA is based on a specific notion and folding of RNA structure. The notion of coarse grained structure employed here is that of canonical RNA pseudoknot contact-structures with at most two mutually crossing bonds (3-noncrossing). These structures are folded by a novel, <it>ab initio </it>prediction algorithm, cross, capable of searching all 3-noncrossing RNA structures. The algorithm outputs the minimum free energy structure.</p> <p>Results</p> <p>After giving some background on RNA pseudoknot structures and providing an outline of the folding algorithm being employed, we present in this paper various, statistical results on the mapping from RNA sequences into 3-noncrossing RNA pseudoknot structures. We study properties, like the fraction of pseudoknot structures, the dominant pseudoknot-shapes, neutral walks, neutral neighbors and local connectivity. We then put our results into context of molecular evolution of RNA.</p> <p>Conclusion</p> <p>Our results imply that, in analogy to RNA secondary structures, 3-noncrossing pseudoknot RNA represents a molecular phenotype that is well suited for molecular and in particular neutral evolution. We can conclude that extended, percolating neutral networks of pseudoknot RNA exist.</p
Salt Effects on the Thermodynamics of a Frameshifting RNA Pseudoknot under Tension
Because of the potential link between -1 programmed ribosomal frameshifting
and response of a pseudoknot (PK) RNA to force, a number of single molecule
pulling experiments have been performed on PKs to decipher the mechanism of
programmed ribosomal frameshifting. Motivated in part by these experiments, we
performed simulations using a coarse-grained model of RNA to describe the
response of a PK over a range of mechanical forces (s) and monovalent salt
concentrations (s). The coarse-grained simulations quantitatively reproduce
the multistep thermal melting observed in experiments, thus validating our
model. The free energy changes obtained in simulations are in excellent
agreement with experiments. By varying and , we calculated the phase
diagram that shows a sequence of structural transitions, populating distinct
intermediate states. As and are changed, the stem-loop tertiary
interactions rupture first, followed by unfolding of the -end
hairpin (). Finally, the
-end hairpin unravels, producing an extended state
(). A theoretical analysis of the phase
boundaries shows that the critical force for rupture scales as with for
() transition. This relation is used to
obtain the preferential ion-RNA interaction coefficient, which can be
quantitatively measured in single-molecule experiments, as done previously for
DNA hairpins. A by-product of our work is the suggestion that the frameshift
efficiency is likely determined by the stability of the -end
hairpin that the ribosome first encounters during translation.Comment: Final draft accepted in Journal of Molecular Biology, 16 pages
including Supporting Informatio
- …