Fish powder as a low-cost component in media for producing bacterial cellulose

Some bacteria can produce extracellular bacterial cellulose (BC). This polysaccharide is chemically identical to cellulose produced by plants but has no associated lignin and hemicelluloses. The unique mechanical properties, chemical stability and purity allow BC to be exploited for a range of biomedical applications. However, medium costs limit commercial BC production. The suitability of using fish powder as a low-cost media component for producing BC by submerged culture of Gluconacetobacter xylinus in shake flasks was investigated. Fish powder was made by drying and grinding Koi carp (Cyprinus carpio), a pest fish in New Zealand waterways. Fermentations were done at 30oC in a growth medium containing 50 g/L glucose, the required minerals, and either 5 g/L yeast extract or 15 g/L fish powder, The BC yield on both yeast extract and fish powder was 0.04 g/g glucose, demonstrating fish powder was a suitable low cost ingredient for supplying nitrogen and amino acids in the media

Decolouring bloodmeal: Consumption and potential recycling of peracetic acid

A method of deodorizing and decolouring bloodmeal using an equilibrium mixture of peracetic acid, hydrogen peroxide, acetic acid and water has been developed to improve its marketability as a source of protein for bioplastics. The objective of this study was to determine what quantity of peracetic acid is required to give reasonable bleaching of the bloodmeal and determine whether there is potential for the wastewater to be recycled. This was carried out by measuring the quantity of chemical species in the initial equilibrium mixture and the resulting wastewater upon bleaching using volumetric analysis. Bleaching efficacy was determined after exposing 100 g bloodmeal to 1.1, 2.5, 3.6, 4.5 and 5.6 wt% peracetic acid solutions as either 300 g total solution or a constant molar equivalent of 2.2 mmol peracetic acid/g bloodmeal and using a chromameter to measure colour change. Addition of 300 g 5.6 wt% peracetic acid solution resulted in effective bleaching. This represented a ratio of 2.20 mmol peracetic acid/g bloodmeal of which 1.4 mmol peracetic acid/g bloodmeal was consumed (63%). If 300 g 300 g of <2.5 wt% solution is added such that there is still 2.2 mmol peracetic acid/g bloodmeal, bleaching is still insufficient. These results suggest that an excess of peracetic is required for bleaching to occur, and that its concentration is paramount to bleaching efficacy. Due to the excess of peracetic acid used in the bleaching process, there is potential for wastewater recycling to be carried out provided that the wastewater is not diluted

An investigation of milk powder deposition on parallel fins

One method to reduce the energy consumption of industrial milk spray dryers is to recover waste heat from the exhaust dryer air. A significant challenge associated with this opportunity is the air contains a small amount of powder that may deposit on the face and surfaces of a recuperator. This paper introduces a novel lab based test that simulates powder deposition on a bank of parallel plate fins at exhaust dryer air conditions. The fin bank acts like the face of a typical finned tube row in a recuperator. The aim of this study is to look at how deposition on the front of fins is affected by the air conditions. Results show similar characteristics to other milk powder deposition studies that exhibit a dramatic increase in deposition once critical stickiness levels are reached. As powder deposits on the face of the fins, the pressure drop across the bank increases until eventually an asymptote occurs, at which point the rates of deposition and removal are similar. For very sticky conditions, deposition on the face of the fins can cause a rise in the pressure drop by as much as 65%. The pressure drop has also been successfully related to the percentage of open frontal area of the fins with and without deposition. Deposition inside and at the rear of the fin bank was found to be minimal

Processibility of corn protein blends and resulting properties of the extrudates

During the last decade, the global biofuels industry has experienced exponential growth. By-products such as high protein corn gluten meal (CGM) and high fibre distillers dried grains with solubles (DDGS) have grown in parallel. CGM has been shown to be suitable as a biopolymer; the high fibre content of DDGS reduces its effectiveness, although it is considerably cheaper. In this study, the processing behaviour of CGM and DDGS blends were evaluated and resulting extrudate properties were determined. Prior to processing, urea was used as a denaturant. DDGS:CGM ratios of 0, 33, 50, 66 and 100% were processed in a single screw extruder, which solely used dissipative heating, with a 2 mm circular die. Resulting screw speeds ranged from 216 to 228 rpm, and die exit temperatures ranged from 96 to 150oC. Blends containing DDGS were less uniformly consolidated and resulted in more dissipative heating. Blends showed multiple glass transitions, which is characteristic of mechanically compatible blends. Transmission electron microscopy revealed phase separation on a micro-scale, although distinct CGM or DDGS phases could not be identified. On a macro-scale, optical microscopy suggested that CGM-rich blends were better consolidated, supported by visual observations of a more continuous extrudate formed during extrusion. As with all biological materials, the extruded blends exhibited sorption behaviour over time, the magnitude of which varied according to blend ratio. EMC values ranged from approximately 0% to nearly 50%, depending upon the humidity level and blend ratio. Nonlinear regression was successfully used to model the effects of relative humidity and blend ratio on the equilibrium moisture contents, with a coefficient of determination of 99%. Future work should aim to also characterize the mechanical properties of these blends to assess their suitability as either bioplastic feedstock or pelletized livestock feed

Structural characterisation of pre-processed thermoplastic protein derived from bloodmeal

Additives are required to convert bloodmeal powder into an extrudable thermoplastic protein-based bioplastic. These include a protein denaturant, a surfactant, a reducing agent and plasticisers. The objective of this work was to assess the structural changes induced in bloodmeal by these additives prior to extrusion. Structure was investigated using Fourier transform infrared (FT-IR) spectroscopy, wide angle X-ray scattering (WAXS) and synchrotron light based FT-IR microspectroscopy. FT-IR results suggested the additives reduced α-helical content. The shape of the amide I region (1600 – 1700 cm⁻¹, representing carbonyl group stretching in the protein backbone) is known to depend on protein secondary structures. Bloodmeal showed a broad, convoluted peak in this region, with a maximum in the range 1648 – 1658 cm⁻¹, associated with α-helices. With processing additives, a dip was seen in the α-helix region, with twin peaks emerging either side of it. Urea, one of the additives, also absorbs in the amide I region and may also contribute to a change in its shape. Analysis of the amide 3 region supported a reduction in the ratio of α helices to β sheets. Further support of structural changes was shown by WAXS. The additives decreased the sharpness of peaks corresponding to 4.8 Å and 10 Å, thought to represent intra-helix spacing and inter-helix packing respectively. FT-IR microspectroscopy at the Australian Synchrotron enabled spatial variations in secondary structure to be explored using peaks in the amide 3 region. Spatial distribution of secondary structure was detected in bloodmeal and thermoplastically modified bloodmeal prior to extrusion (PPM-TEG). Bloodmeal showed domain separation on the approximate order of 10 μm, whilst PPM-TEG appeared to have larger phases and overall reduced α-helical content, relative to beta sheets

Corporate sustainability reporting index and baseline data for the cruise industry

Sustainability policies and corporate reports demonstrate the impacts cruise companies acknowledge as their responsibility, and the actions put in place to address them. This paper develops a corporate social responsibility index based on the Global Reporting Initiative, with industry specific additions including labor and human rights, health and safety, and environmental and economic aspects. Companies disclose more management than performance data, which is typical of early stages of development. Companies disclosing less information focus on soft indicators which are easy to mimic and demonstrate posturing. Items disclosed tend to be marginal to the core of the business, have a positive economic impact or pre-empt sector regulation. Reports echo the voice of the corporations and not the demands of stakeholders. Institutional isomorphism has not influenced a homogenization in reporting, with only the largest firms reporting at this stage

The global hydrology education resource

This article is a selective overview of a range of contemporary teaching resources currently available globally for university hydrology educators, with an emphasis on web-based resources. Major governmental and scientific organizations relevant to the promotion of hydrology teaching are briefly introduced. Selected online teaching materials are then overviewed, i.e. PowerPoint presentations, course materials, and multimedia. A range of websites offering free basic hydrology modelling software are mentioned, together with some data file sources which could be used for teaching. Websites offering a considerable range of general hydrology links are also noted, as are websites providing international and national data sets which might be incorporated into teaching exercises. Finally, some discussion is given on reference material for different modes of hydrology teaching, including laboratory and field exercises

Discrete rate maximisation power allocation with enhanced BER

This study aims to maximise the rate over a multiple-in multiple-out (MIMO) link using incremental power and bit allocation. Two different schemes, greedy power allocation (GPA) and greedy bit allocation (GBA), are addressed and compared with the standard uniform power allocation (UPA). The design is constrained by the target bit error ratio (BER), the total power budget and fixed discrete modulation orders. The authors demonstrate through simulations that GPA outperforms GBA in terms of throughput and power conservation, whereas GBA is advantageous when a lower BER is beneficial. Once the design constraints are satisfied, remaining power is utilised in two possible ways, leading to improved performance of GPA and UPA algorithms. This redistribution is analysed for fairness in BER performance across all active subchannels using a bisection method