A new transgenic reporter line reveals Wnt-dependent Snai2 re-expression and cranial neural crest differentiation in Xenopus

During vertebrate embryogenesis, the cranial neural crest (CNC) forms at the neural plate border and subsequently migrates and differentiates into many types of cells. The transcription factor Snai2, which is induced by canonical Wnt signaling to be expressed in the early CNC, is pivotal for CNC induction and migration in Xenopus. However, snai2 expression is silenced during CNC migration, and its roles at later developmental stages remain unclear. We generated a transgenic X. tropicalis line that expresses enhanced green fluorescent protein (eGFP) driven by the snai2 promoter/enhancer, and observed eGFP expression not only in the pre-migratory and migrating CNC, but also the differentiating CNC. This transgenic line can be used directly to detect deficiencies in CNC development at various stages, including subtle perturbation of CNC differentiation. In situ hybridization and immunohistochemistry confirm that Snai2 is re-expressed in the differentiating CNC. Using a separate transgenic Wnt reporter line, we show that canonical Wnt signaling is also active in the differentiating CNC. Blocking Wnt signaling shortly after CNC migration causes reduced snai2 expression and impaired differentiation of CNC-derived head cartilage structures. These results suggest that Wnt signaling is required for snai2 re-expression and CNC differentiation

Application of the Titius-Bode Rule to the 55 Cancri System: Tentative Prediction of a Possibly Habitable Planet

Following the notion that the Titius-Bode rule (TBR) may also be applicable to some extra-solar planetary systems, although this number could be relatively small, it is applied to 55 Cancri, which is a G-type main-sequence star currently known to host five planets. Following a concise computational process, we tentatively identify four new hypothetical planetary positions given as 0.081, 0.41, 1.51 and 2.95 AU from the star. The likelihood that these positions are occupied by real existing planets is significantly enhanced for the positions of 1.51 and 2.95 AU in the view of previous simulations on planet formation and planetary orbital stability. For example, Raymond et al. (2008) [ApJ 689, 478] argued that additional planets would be possible between 55 Cnc f and 55 Cnc d, which would include planets situated at 1.51 and 2.95 AU. If two additional planets are assumed to exist between 55 Cnc f and 55 Cnc d, the deduced domains of stability would be given as 1.3-1.6 and 2.2-3.3 AU. The possible planet near 1.5 AU appears to be located at the outskirts of the stellar habitable zone, which is however notably affected by the stellar parameters as well as the adopted model of circumstellar habitability. We also compute the distance of the next possible outer planet in the 55 Cnc system, which if existing is predicted to be located between 10.9 and 12.2 AU, which is consistent with orbital stability constraints. The inherent statistical significance of the TBR is evaluated following the method by Lynch (2003) [MNRAS 341, 1174]. Yet it is up to future planetary search missions to verify or falsify the applicability of the TBR to the 55 Cnc system, and to attain information on additional planets, if existing.Comment: 9 pages, 3 figures, 9 tables; accepted by Publications of the Astronomical Society of Japan; to be published on 2012 August 25; Ref.: PASJ 64 (4

Lorentz invariance violation and charge (non--)conservation: A general theoretical frame for extensions of the Maxwell equations

All quantum gravity approaches lead to small modifications in the standard laws of physics which lead to violations of Lorentz invariance. One particular example is the extended standard model (SME). Here, a general phenomenological approach for extensions of the Maxwell equations is presented which turns out to be more general than the SME and which covers charge non--conservation (CNC), too. The new Lorentz invariance violating terms cannot be probed by optical experiments but need, instead, the exploration of the electromagnetic field created by a point charge or a magnetic dipole. Some scalar--tensor theories and higher dimensional brane theories predict CNC in four dimensions and some models violating Special Relativity have been shown to be connected with CNC and its relation to the Einstein Equivalence Principle has been discussed. Due to this upcoming interest, the experimental status of electric charge conservation is reviewed. Up to now there seem to exist no unique tests of charge conservation. CNC is related to the precession of polarization, to a modification of the $1/r$--Coulomb potential, and to a time-dependence of the fine structure constant. This gives the opportunity to describe a dedicated search for CNC.Comment: To appear in Physical Review