Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part I: Cellular characterization

Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for regenerative medicine, as they are a patient-specific and histocompatible cell source for the treatment of varying diseases. However, currently, little is known about their cellular and molecular profile. In the present study, in a mouse model different donor cell-derived ntESCs from various genetic backgrounds were compared with reference ESCs and analyzed comprehensively at the cellular level. A number of pluripotency marker genes were compared by flow cytometry and immunocytochemistry analysis. Significant differences at the protein level were observed for POU5F1, SOX2, FGF4, NANOG, and SSEA-1. However, such differences had no effect on in vitro cell differentiation and cell fate: derivatives of the three germ layers were detected in all ntESC lines. The neural and cardiac in vitro differentiation revealed minor differences between the cell lines, both at the mRNA and protein level. Karyotype analyses and cell growth studies did not reveal any significant variations. Despite some differences observed, the present study revealed that ntESC lines had similar differentiation competences compared to other ESCs. The results indicate that the observed differences may be related to the genotype rather than to the nuclear transfer technology

Moduli space actions on the Hochschild Co-Chains of a Frobenius algebra I: Cell Operads

This is the first of two papers in which we prove that a cell model of the moduli space of curves with marked points and tangent vectors at the marked points acts on the Hochschild co--chains of a Frobenius algebra. We also prove that a there is dg--PROP action of a version of Sullivan Chord diagrams which acts on the normalized Hochschild co-chains of a Frobenius algebra. These actions lift to operadic correlation functions on the co--cycles. In particular, the PROP action gives an action on the homology of a loop space of a compact simply--connected manifold. In this first part, we set up the topological operads/PROPs and their cell models. The main theorems of this part are that there is a cell model operad for the moduli space of genus $g$ curves with $n$ punctures and a tangent vector at each of these punctures and that there exists a CW complex whose chains are isomorphic to a certain type of Sullivan Chord diagrams and that they form a PROP. Furthermore there exist weak versions of these structures on the topological level which all lie inside an all encompassing cyclic (rational) operad.Comment: 50 pages, 7 figures. Newer version has minor changes. Some material shifted. Typos and small things correcte

GRADING DISCREPANCY IN GLOBAL EDUCATION

This research paper explores the history, ideas and relevance of grading in the globalized world and discusses if grades are a proficient tool to measure proficiency in academic disciplines. It investigates finding based on a simple experiment, which shows grave differences in grading worldwide. The idea of grading was implemented in the eighteen century and this research explores if a system that is based on national measurement has relevance in the globalized world. This research examines the relationship between grades teachers give and proficiency scores given to the same students by external raters. This research found little comparison between teachers’ grading system and student proficiency. The research concludes that the current grading systems are of little to no use in the general measurement of proficiency of academic disciplines. It is the recommendation of the research that new measures are implemented and a new system not only based on proficiency skills are used to measure academic excellence

Tiktok Influences on Teenagers and Young Adults Students: The Common Usages of the Application Tiktok

This research paper is aimed at shedding light on the use of the mobile application Tiktok, it concluded that the use of the application, has had a very little negative impact on the main target user base. The research outlines to a certain degree some difficulties in the management of the application but in comparison with similar social media applications, they appear to be few. This research has concluded that the use of the application seems to be of more concern to spectators of the users than the actual users. This research has also been able to map out the spread of Tiktok over the past 3 years and how it has become a significant participant in the role of influencers, creating an entrepreneurial possibility with its growth. This research recommends further research into the use of popular social media apps and further monitoring of the expansion of the popularity of the usages of social media apps in general

Charismatic Heads of State: The Social Context

http://deepblue.lib.umich.edu/bitstream/2027.42/50843/1/61.pd

Letter from the Friars Cell and Providence College Theatre Department to the Dominican Fathers and Brothers

Providence College Department of Theatre, Dance & Film Letter from the Friars Cell and Providence College Theatre Department to the Dominican Fathers and Brothers regarding Who is Chasing Whom, Sonny DeRee\u27s Life Flashes Before His Eyes, and Midlife Crisis of Dionysus. November 18, 1997https://digitalcommons.providence.edu/experiments_comedy_1997_pubs/1004/thumbnail.jp

Memory NK cell features exploitable in anticancer immunotherapy

Besides their innate ability to rapidly produce effector cytokines and kill virus-infected or transformed cells, natural killer (NK) cells display a strong capability to adapt to environmental modifications and to differentiate into long-lived, hyperfunctional populations, dubbed memory or memory-like NK cells. Despite significant progress in the field of NK cell-based immunotherapies, some factors including their short life span and the occurrence of a tumor-dependent functional exhaustion have limited their clinical efficacy so that strategies aimed at overcoming these limitations represent one of the main current challenges in the field. In this scenario, the exploitation of NK cell memory may have a considerable potential. This article summarizes recent evidence in the literature on the peculiar features that render memory NK cells an attractive tool for antitumor immunotherapy, including their long-term survival and in vivo persistence, the resistance to tumor-dependent immunosuppressive microenvironment, the amplified functional responses to IgG-opsonized tumor cells, and in vitro expansion capability. Along with highlighting these issues, we speculate that memory NK cell-based adoptive immunotherapy settings would greatly take advantage from the combination with tumor-targeting therapeutic antibodies (mAbs), as a strategy to fully unleash their clinical efficacy

Relationship and predictive role of the dual expression of FGFR and IL-8 in metastatic renal cell carcinoma treated with targeted agents

Background/Aim: The expression of IL-8 and FGFR has been related to prognosis and pathological features in renal cell carcinoma. We investigated the relationship between IL-8 and FGFR and the outcome in metastatic renal cell carcinoma (mRCC) patients. Materials and Methods: Clinical data and histological samples of patients affected by mRCC and treated with targeted agents were reviewed. The expression of proteins was assessed using immunohistochemistry. Results: FGFR1, FGFR2, and IL-8 were found to be expressed in 16%, 30%, and 50% of cases, respectively. Significant correlations were found between selected proteins. A lack of expression of FGFR2 and IL8 was found to be correlated with increased progression-free survival (PFS). The survival rate at 24 months was 44%, 38%, and 79% of those expressing both, one, or none of the evaluated proteins, respectively (p=0.047). Conclusion: This analysis found a relationship between the expression of IL-8 and FGFR2 in mRCC patients treated with targeted agents

Letter from The Friars Cell and Providence College Theatre Department to Dominican Sisters

Providence College Department of Theatre, Dance & Film Letter from the Friars Cell and Providence College Theatre Department to the Dominican Sisters regarding Who is Chasing Whom, Sonny DeRee\u27s Life Flashes Before His Eyes, and Midlife Crisis of Dionysus. November 18, 1997https://digitalcommons.providence.edu/experiments_comedy_1997_pubs/1005/thumbnail.jp