4,876 research outputs found

    A simple iterative independent component analysis algorithm for vibration source signal identification of complex structures

    Get PDF
    ABSTRACT:Independent Component Analysis (ICA), one of the blind source separation methods, can be applied for extracting unknown source signals only from received signals. This is accomplished by finding statistical independence of signal mixtures and has been successfully applied to myriad fields such as medical science, image processing, and numerous others. Nevertheless, there are inherent problems that have been reported when using this technique: insta- bility and invalid ordering of separated signals, particularly when using a conventional ICA technique in vibratory source signal identification of complex structures. In this study, a simple iterative algorithm of the conventional ICA has been proposed to mitigate these problems. The proposed method to extract more stable source signals having valid order includes an iterative and reordering process of extracted mixing matrix to reconstruct finally converged source signals, referring to the magnitudes of correlation coefficients between the intermediately separated signals and the signals measured on or nearby sources. In order to review the problems of the conventional ICA technique and to vali- date the proposed method, numerical analyses have been carried out for a virtual response model and a 30m class submarine model. Moreover, in order to investigate applicability of the proposed method to real problem of complex structure, an experiment has been carried out for a scaled submarine mockup. The results show that the proposed method could resolve the inherent problems of a conventional ICA technique

    Blind Deconvolution of Anisoplanatic Images Collected by a Partially Coherent Imaging System

    Get PDF
    Coherent imaging systems offer unique benefits to system operators in terms of resolving power, range gating, selective illumination and utility for applications where passively illuminated targets have limited emissivity or reflectivity. This research proposes a novel blind deconvolution algorithm that is based on a maximum a posteriori Bayesian estimator constructed upon a physically based statistical model for the intensity of the partially coherent light at the imaging detector. The estimator is initially constructed using a shift-invariant system model, and is later extended to the case of a shift-variant optical system by the addition of a transfer function term that quantifies optical blur for wide fields-of-view and atmospheric conditions. The estimators are evaluated using both synthetically generated imagery, as well as experimentally collected image data from an outdoor optical range. The research is extended to consider the effects of weighted frame averaging for the individual short-exposure frames collected by the imaging system. It was found that binary weighting of ensemble frames significantly increases spatial resolution

    Automated Analysis of Fluorescent Microscopic Images to Identify Protein-Protein Interactions

    Get PDF
    The identification and confirmation of protein interactions significantly challenges the field of systems biology and related bio-computational efforts. The identification of protein-protein interactions along with their spatial and temporal localization is useful for assigning functional information to proteins. Fluorescence microscopy is an ideal method for assessing protein localization and interactions as a number of techniques and reagents have been described. Historically, data sets obtained from fluorescence microscopy have been analyzed manually, a process that is both time consuming and tedious. The development of an automated system that can measure the location and dynamics of interacting proteins inside a live cell is of high priority. This paper describes an automated image analysis system used to identify an interaction between two proteins of interest. These proteins are fused to either Green Fluorescent Protein (GFP) or DivIVA, a bacterial cell division protein that localizes to the cell poles. Upon induction of the DivIVA fusion protein, the GFP-fusion protein is recruited to the cell poles if a positive interaction occurs. There were many problems that came into the picture during the development for an automated system to identify these positive interactions. There were basic segmentation and edge detection problems and the problems caused by inclusion bodies (will be discussed in the sections to follow). Different known procedures to obtain thresholds, and edges were evaluated and the apt ones for our analysis were implemented. A proper flow of advanced image processing and feature extraction algorithms was laid out. These steps were used to analyze the datasets of acquired images. Various methods applied are discussed in detail. The experiments conducted along with the results generated are discussed extensively. A statistical feature set used to quantify the image based information and to aid in the determination of a positive interaction is developed. Various image processing and feature extraction algorithms used to analyze fluorescence microscopic images were also applied to Atomic force microscopic images with a few modifications. There was a basic problem of uneven background noise and this was removed using a common procedure that is used to remove uneven illumination in DIC images. These AFM images were analyzed and quantized using numerical descriptors defined during the analysis of fluorescent microscopic images
    • 

    corecore