Surface plasmon resonance photonic biosensors based on phase-sensitive measurement techniques.

Law Wing Cheung.Thesis (M.Phil.)--Chinese University of Hong Kong, 2005.Includes bibliographical references.Abstracts in English and Chinese.Abstract --- p.IAcknowledgements --- p.VList of Publications related to this project --- p.VIContents --- p.VIIChapter Chapter 1 --- Introduction --- p.1-1Chapter Chapter 2 --- Literature ReviewChapter 2.1 --- Surface Plasmon Waves --- p.2-2Chapter 2.2 --- Excitation of Surface Plasmon --- p.2-4Chapter 2.2.1 --- Surface Plasmon Coupling Schemes --- p.2-6Chapter 2.3 --- Detection Techniques used in SPR sensors --- p.2-13Chapter 2.3.1 --- Angular Interrogation --- p.2-14Chapter 2.3.2 --- Wavelength Interrogation --- p.2-15Chapter 2.3.3 --- Intensity Interrogation --- p.2-16Chapter 2.3.4 --- Phase Interrogation --- p.2-16Chapter 2.3.5 --- Commercial SPR biosensors --- p.2-18Chapter 2.3.6 --- Comparison between Detection Techniques --- p.2-19Chapter 2.4 --- Applications of SPR biosensors --- p.2-21Chapter Chapter 3 --- Principle of Surface Plasmon Resonance Sensing TechnologyChapter 3.1 --- SPR Phenomenon --- p.3-1Chapter 3.2 --- Conditions for Surface Plasmon Resonance --- p.3-5Chapter 3.3 --- Wave-vectors --- p.3-7Chapter 3.4 --- Surface Plasmon Resonance described by Fresnel's Theory --- p.3-8Chapter 3.5 --- Concept of Surface Plasmon Resonance Biosensing --- p.3-10Chapter Chapter 4 --- ExperimentsChapter 4.1 --- Highly sensitive differential phase-sensitive surface plasmon resonance biosensor based on Mach-Zehnder configuration --- p.4-1Chapter 4.1.1 --- Materials required --- p.4-1Chapter 4.1.2 --- Experimental Setup --- p.4-2Chapter 4.1.3 --- Principle of Differential Phase Measurement --- p.4-3Chapter 4.1.4 --- Photodetector Circuitry --- p.4-6Chapter 4.1.5 --- Digital Signal Processing --- p.4-7Chapter 4.1.6 --- Polymer based Micro-fluidic System Integrated with SPR Biosensor --- p.4-9Chapter 4.2 --- Phase-sensitive Surface Plasmon Resonance Biosensor using the Photoelastic Modulation Technique --- p.4-12Chapter 4.2.1 --- Materials required --- p.4-12Chapter 4.2.2 --- Experimental Setup --- p.4-13Chapter 4.2.3 --- Principle of Photoelastic Modulation Technique and Signal Processing --- p.4-14Chapter 4.2.4 --- Operation Principle of Photoelastic Modulator --- p.4-17Chapter 4.3 --- Sample Preparations --- p.4-18Chapter 4.3.1 --- Glycerin-water Mixtures --- p.4-18Chapter 4.3.2 --- "PBS, BSA and BSA antibody" --- p.4-19Chapter 4.3.3 --- "RPMI, Trypsin, Cells and SDS" --- p.4-20Chapter Chapter5 --- Results amd DiscussionsChapter 5.1 --- Experimental setup I: Highly sensitive differential phase-sensitive surface plasmon resonance biosensor based on Mach-Zehnder configuration --- p.5-1Chapter 5.1.1 --- Measuring various glycerin-water concentration mixture with silver-gold sensing layer --- p.5-1Chapter 5.1.2 --- Comparison between the sensitivity of our setup and reported setup based on phase detection --- p.5-4Chapter 5.1.3 --- Discussion on 0.01° system resolution --- p.5-7Chapter 5.1.4 --- Experiment on monitoring BSA-BSA antibody binding reaction --- p.5-9Chapter 5.1.5 --- Matching oil and glass slide --- p.5-11Chapter 5.1.6 --- Experiments on monitoring BSA-BSA antibody binding reaction with integrated microfluidic system --- p.5-12Chapter 5.1.7 --- Experiment on observing cell adhesion properties on gold surface under the influence of trypsin --- p.5-14Chapter 5.1.8 --- Discussion on the non-specific binding between trypsin and gold surface --- p.5-16Chapter 5.1.9 --- Modifying the gold surface with BSA layer --- p.5-17Chapter 5.1.10 --- Experiment on observing cell adhesion properties on the gold surface under the influence Sodium Dodecyl Sulfate (SDS) --- p.5-18Chapter 5.2 --- Experimental setup II: Phase-sensitive surface plasmon resonance biosensor using the photoelastic modulation technique --- p.5-21Chapter 5.2.1 --- Measurement on difference glycerin-water concentration mixture --- p.5-21Chapter 5.2.2 --- Experiment on monitoring BSA-BSA antibody binding reaction --- p.5-23Chapter Chapter 6 --- Conclusions and Future WorksChapter 6.1 --- Conclusions --- p.6-1Chapter 6.2 --- Future Works --- p.6-2References --- p.R-1AppendixChapter A. --- Phase Extraction Routine written by Matlab --- p.A-1Chapter B. --- Mathematical expressions for calculating the phase angle in the experiment of SPR biosensor using the Photoelastic Modulation Technique --- p.A-6Chapter C. --- Relationship between Concentration and Refractive Index of Glycerin-Water Mixture --- p.A-11Chapter D. --- Physical Properties of Bovine Serum Albumin --- p.A-12Chapter E. --- Simulation Curve written by Matlab --- p.A-1

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