A Study of Raman Spectroscopy as a Clinical Diagnostic Tool for the Detection of Lynch Syndrome/Hereditary NonPolyposis Colorectal Cancer (HNPCC)

Lynch syndrome also known as hereditary non-polyposis colorectal cancer (HNPCC) is a highly penetrant hereditary form of colorectal cancer that accounts for approximately 3% of all cases. It is caused by mutations in DNA mismatch repair resulting in accelerated adenoma to carcinoma progression. The current clinical guidelines used to identify Lynch Syndrome (LS) are known to be too stringent resulting in overall underdiagnoses. Raman spectroscopy is a powerful analytical tool used to probe the molecular vibrations of a sample to provide a unique chemical fingerprint. The potential of using Raman as a diagnostic tool for discriminating LS from sporadic adenocarcinoma is explored within this thesis. A number of experimental parameters were initially optimized for use with formalin fixed paraffin embedded colonic tissue (FFPE). This has resulted in the development of a novel cost-effective backing substrate shown to be superior to the conventionally used calcium fluoride (CaF2). This substrate is a form of silanized super mirror stainless steel that was found to have a much lower Raman background, enhanced Raman signal and complete paraffin removal from FFPE tissues. Performance of the novel substrate was compared against CaF2 by acquiring large high resolution Raman maps from FFPE rat and human colonic tissue. All of the major histological features were discerned from steel mounted tissue with the benefit of clear lipid signals without paraffin obstruction. Biochemical signals were comparable to those obtained on CaF2 with no detectable irregularities. By using principal component analysis to reduce the dimensionality of the dataset it was then possible to use linear discriminant analysis to build a classification model for the discrimination of normal colonic tissue (n=10) from two pathological groups: LS (n=10) and sporadic adenocarcinoma (n=10). Using leaveone-map-out cross-validation of the model classifier has shown that LS was predicted with a sensitivity of 63% and a specificity of 89% - values that are competitive with classification techniques applied routinely in clinical practice

Ultrasound renal stone diagnosis based on convolutional neural network and VGG16 features

This paper deals with the classification of the kidneys for renal stones on ultrasound images. Convolutional neural network (CNN) and pre-trained CNN (VGG16) models are used to extract features from ultrasound images. Extreme gradient boosting (XGBoost) classifiers and random forests are used for classification. The features extracted from CNN and VGG16 are used to compare the performance of XGBoost and random forest. An image with normal and renal stones was classified. This work uses 630 real ultrasound images from Al-Diwaniyah General Teaching Hospital (a lithotripsy center) in Iraq. Classifier performance is evaluated using its accuracy, recall, and F1 score. With an accuracy of 99.47%, CNN-XGBoost is the most accurate model

Automatic emotion recognition in clinical scenario: a systematic review of methods

none4Automatic emotion recognition has powerful opportunities in the clinical field, but several critical aspects are still open, such as heterogeneity of methodologies or technologies tested mainly on healthy people. This systematic review aims to survey automatic emotion recognition systems applied in real clinical contexts, to deeply analyse clinical and technical aspects, how they were addressed, and relationships among them. The literature review was conducted on: IEEEXplore, ScienceDirect, Scopus, PubMed, ACM. Inclusion criteria were the presence of an automatic emotion recognition algorithm and the enrollment of at least 2 patients in the experimental protocol. The review process followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Moreover, the works were analysed according to a reference model to deeply examine both clinical and technical topics. 52 scientific papers passed inclusion criteria. Most clinical scenarios involved neurodevelopmental, neurological and psychiatric disorders with the aims of diagnosing, monitoring, or treating emotional symptoms. The most adopted signals are video and audio, while supervised shallow learning is mostly used for emotion recognition. A poor study design, tiny samples, and the absence of a control group emerged as methodological weaknesses. Heterogeneity of performance metrics, datasets and algorithms challenges results comparability, robustness, reliability and reproducibility.openPepa, Lucia; Spalazzi, Luca; Capecci, Marianna; Ceravolo, Maria GabriellaPepa, Lucia; Spalazzi, Luca; Capecci, Marianna; Ceravolo, Maria Gabriell

Glomerular proteomic profiling of kidney biopsies with hypertensive nephropathy reveals a signature of disease progression

Hypertensive nephropathy (HN) requires a kidney biopsy as diagnostic gold-standard but histological findings are unspecific and specific prognostic markers are missing. We aimed at identifying candidate prognostic markers based on glomerular protein signatures. We studied adult patients (n = 17) with eGFR >30 ml/min/1.73m(2) and proteinuria = 1.5, pPeer reviewe

Machine Learning Techniques for Differential Diagnosis of Vertigo and Dizziness: A Review.

Vertigo is a sensation of movement that results from disorders of the inner ear balance organs and their central connections, with aetiologies that are often benign and sometimes serious. An individual who develops vertigo can be effectively treated only after a correct diagnosis of the underlying vestibular disorder is reached. Recent advances in artificial intelligence promise novel strategies for the diagnosis and treatment of patients with this common symptom. Human analysts may experience difficulties manually extracting patterns from large clinical datasets. Machine learning techniques can be used to visualize, understand, and classify clinical data to create a computerized, faster, and more accurate evaluation of vertiginous disorders. Practitioners can also use them as a teaching tool to gain knowledge and valuable insights from medical data. This paper provides a review of the literatures from 1999 to 2021 using various feature extraction and machine learning techniques to diagnose vertigo disorders. This paper aims to provide a better understanding of the work done thus far and to provide future directions for research into the use of machine learning in vertigo diagnosis

Application of infrared thermography in computer aided diagnosis

The invention of thermography, in the 1950s, posed a formidable problem to the research community: What is the relationship between disease and heat radiation captured with Infrared (IR) cameras? The research community responded with a continuous effort to find this crucial relationship. This effort was aided by advances in processing techniques, improved sensitivity and spatial resolution of thermal sensors. However, despite this progress fundamental issues with this imaging modality still remain. The main problem is that the link between disease and heat radiation is complex and in many cases even non-linear. Furthermore, the change in heat radiation as well as the change in radiation pattern, which indicate disease, is minute. On a technical level, this poses high requirements on image capturing and processing. On a more abstract level, these problems lead to inter-observer variability and on an even more abstract level they lead to a lack of trust in this imaging modality. In this review, we adopt the position that these problems can only be solved through a strict application of scientific principles and objective performance assessment. Computing machinery is inherently objective; this helps us to apply scientific principles in a transparent way and to assess the performance results. As a consequence, we aim to promote thermography based Computer-Aided Diagnosis (CAD) systems. Another benefit of CAD systems comes from the fact that the diagnostic accuracy is linked to the capability of the computing machinery and, in general, computers become ever more potent. We predict that a pervasive application of computers and networking technology in medicine will help us to overcome the shortcomings of any single imaging modality and this will pave the way for integrated health care systems which maximize the quality of patient care

ATEM: A Topic Evolution Model for the Detection of Emerging Topics in Scientific Archives

This paper presents ATEM, a novel framework for studying topic evolution in scientific archives. ATEM is based on dynamic topic modeling and dynamic graph embedding techniques that explore the dynamics of content and citations of documents within a scientific corpus. ATEM explores a new notion of contextual emergence for the discovery of emerging interdisciplinary research topics based on the dynamics of citation links in topic clusters. Our experiments show that ATEM can efficiently detect emerging cross-disciplinary topics within the DBLP archive of over five million computer science articles

Big data analytics for preventive medicine

© 2019, Springer-Verlag London Ltd., part of Springer Nature. Medical data is one of the most rewarding and yet most complicated data to analyze. How can healthcare providers use modern data analytics tools and technologies to analyze and create value from complex data? Data analytics, with its promise to efficiently discover valuable pattern by analyzing large amount of unstructured, heterogeneous, non-standard and incomplete healthcare data. It does not only forecast but also helps in decision making and is increasingly noticed as breakthrough in ongoing advancement with the goal is to improve the quality of patient care and reduces the healthcare cost. The aim of this study is to provide a comprehensive and structured overview of extensive research on the advancement of data analytics methods for disease prevention. This review first introduces disease prevention and its challenges followed by traditional prevention methodologies. We summarize state-of-the-art data analytics algorithms used for classification of disease, clustering (unusually high incidence of a particular disease), anomalies detection (detection of disease) and association as well as their respective advantages, drawbacks and guidelines for selection of specific model followed by discussion on recent development and successful application of disease prevention methods. The article concludes with open research challenges and recommendations