Automated Discrimination of Pathological Regions in Tissue Images: Unsupervised Clustering vs Supervised SVM Classification

Recognizing and isolating cancerous cells from non pathological tissue areas (e.g. connective stroma) is crucial for fast and objective immunohistochemical analysis of tissue images. This operation allows the further application of fully-automated techniques for quantitative evaluation of protein activity, since it avoids the necessity of a preventive manual selection of the representative pathological areas in the image, as well as of taking pictures only in the pure-cancerous portions of the tissue. In this paper we present a fully-automated method based on unsupervised clustering that performs tissue segmentations highly comparable with those provided by a skilled operator, achieving on average an accuracy of 90%. Experimental results on a heterogeneous dataset of immunohistochemical lung cancer tissue images demonstrate that our proposed unsupervised approach overcomes the accuracy of a theoretically superior supervised method such as Support Vector Machine (SVM) by 8%

Steganoscription : exploring techniques for privacy-preserving crowdsourced transcription of handwritten documents

textThe focus my research is the historical document format represented by the Central State Hospital (CSH) dataset, handwritten medical records. The specific problem innate to the CSH dataset in question is how to transcribe sensitive, cursive-handwritten documents via a manual vehicle- such as crowdsourcing. Manual methods are necessarily no matter the sophistication of the optical character recognition system used because of the inconsistencies within cursive script. To address this problem I've developed an application that enables users to transcribe sensitive, handwritten, document images while preserving the privacy of the context around the transcribed text via random word selection and visual manipulation of the displayed text. This is made possible through several algorithms that process documents from a top-down approach. These system operations detect and segment lines of text in images, reverse the slant common to cursive script, detect and segment words, and finally, manipulate word-images before they are displayed to users; combinations of color, noise, and geometric manipulations are currently supported and used randomly. This system, called Steganoscription, combines the concepts of steganography and transcription.Informatio

Adaptive Algorithms for Automated Processing of Document Images

Large scale document digitization projects continue to motivate interesting document understanding technologies such as script and language identification, page classification, segmentation and enhancement. Typically, however, solutions are still limited to narrow domains or regular formats such as books, forms, articles or letters and operate best on clean documents scanned in a controlled environment. More general collections of heterogeneous documents challenge the basic assumptions of state-of-the-art technology regarding quality, script, content and layout. Our work explores the use of adaptive algorithms for the automated analysis of noisy and complex document collections. We first propose, implement and evaluate an adaptive clutter detection and removal technique for complex binary documents. Our distance transform based technique aims to remove irregular and independent unwanted foreground content while leaving text content untouched. The novelty of this approach is in its determination of best approximation to clutter-content boundary with text like structures. Second, we describe a page segmentation technique called Voronoi++ for complex layouts which builds upon the state-of-the-art method proposed by Kise [Kise1999]. Our approach does not assume structured text zones and is designed to handle multi-lingual text in both handwritten and printed form. Voronoi++ is a dynamically adaptive and contextually aware approach that considers components' separation features combined with Docstrum [O'Gorman1993] based angular and neighborhood features to form provisional zone hypotheses. These provisional zones are then verified based on the context built from local separation and high-level content features. Finally, our research proposes a generic model to segment and to recognize characters for any complex syllabic or non-syllabic script, using font-models. This concept is based on the fact that font files contain all the information necessary to render text and thus a model for how to decompose them. Instead of script-specific routines, this work is a step towards a generic character and recognition scheme for both Latin and non-Latin scripts

Automated image segmentation for detecting cell spreading for metastasizing assessments of cancer development

The automated segmentation of cells in microscopic images is an open research problem that has important implications for studies of the developmental and cancer processes based on in vitro models. In this paper, we present the approach for segmentation of the DIC images of cultured cells using G-neighbor smoothing followed by Kauwahara filtering and local standard deviation approach for boundary detection. NIH FIJI/ImageJ tools are used to create the ground truth dataset. The results of this work indicate that detection of cell boundaries using segmentation approach even in the case of realistic measurement conditions is a challenging problem

A Fuzzy Approach to Text Segmentation in Web Images Based on Human Colour Perception

This chapter describes a new approach for the segmentation of text in images on Web pages. In the same spirit as the authors’ previous work on this subject, this approach attempts to model the ability of humans to differentiate between colours. In this case, pixels of similar colour are first grouped using a colour distance defined in a perceptually uniform colour space (as opposed to the commonly used RGB). The resulting colour connected components are then grouped to form larger (character-like) regions with the aid of a propinquity measure, which is the output of a fuzzy inference system. This measure expresses the likelihood for merging two components based on two features. The first feature is the colour distance between the components, in the L*a*b* colour space. The second feature expresses the topological relationship of two components. The results of the method indicate a better performance than previous methods devised by the authors and possibly better (a direct comparison is not really possible due to the differences in application domain characteristics between this and previous methods) performance to other existing methods

Image Analysis Based on Soft Computing and Applied on Space Shuttle During the Liftoff Process

Imaging techniques based on Soft Computing (SC) and developed at Kennedy Space Center (KSC) have been implemented on a variety of prototype applications related to the safety operation of the Space Shuttle during the liftoff process. These SC-based prototype applications include detection and tracking of moving Foreign Objects Debris (FOD) during the Space Shuttle liftoff, visual anomaly detection on slidewires used in the emergency egress system for the Space Shuttle at the laJlIlch pad, and visual detection of distant birds approaching the Space Shuttle launch pad. This SC-based image analysis capability developed at KSC was also used to analyze images acquired during the accident of the Space Shuttle Columbia and estimate the trajectory and velocity of the foam that caused the accident