881 research outputs found

    An investigation into mechanisms of regeneration specificity in planarian flatworms.

    Get PDF
    Many animals have the extraordinary ability to replace lost body parts, even so we humans do not. One critical but poorly understood aspect of this phenomenon is how wounds tailor the regeneration response to the particular target structure that needs to be regrown. In my thesis work I have attempted to address this problem in the champions of regeneration, the planarian flatworms. If one of these animals is cut into tiny pieces, each of the pieces will regenerate a head at the anterior end and tail at the posterior end. For over a century investigators have searched for the intrinsic polarity cue underlying this regeneration polarity, but until now its mechanistic basis is not known. The explicit goal of my thesis work was to identify this cue. The general approach that I have taken toward identification of the intrinsic polarity is to systematically compare two different planarian species with subtle variations in the establishment of regeneration polarity, Schmidtea mediterranea and Girardia tigrina. First, I demonstrate through systematic comparison of different amputation paradigms that regeneration polarity is dependent not only on species, but also on piece length, body size and anteroposterior axis position. Second, given that these findings are consistent with a gradient- based intrinsic polarity cue as prevalent hypothesis in the field, I tested whether the recently identified tail-to-head gradient of canonical Wnt (cWnt) signalling could be mechanistic basis of regeneration polarity. As precondition for doing so, I developed new approaches to measure and manipulate cWnt signalling in planaria. The data acquired with these tools suggest that the cWnt gradient may contribute to the observed position-dependence of regeneration polarity but is overall not the (only) intrinsic polarity cue. Third, I present my initial efforts to test whether the longitudinal muscle fibres (LMFs) in which notum is exclusively activated are an intrinsic polarity cue. My results suggest that “bundles” of short, intrinsically polarised LMFs running along the AP axis may express notum when they are cut anterior to their nucleus and moreover that misregulation of such a mechanism may underlie the species-dependence of regeneration polarity. Overall, the work presented in this thesis offers new insight into the cellular and conceptual basis of planarian regeneration polarity and, in doing so, the more general question of how regenerative organisms “sense” precisely what body part is missing and therefore needs to be regrown. Furthermore, it puts forward new hypotheses that through additional experimentation may explain lead to elucidation of the underlying molecular mechanisms

    Comparing the production of a formula with the development of L2 competence

    Get PDF
    This pilot study investigates the production of a formula with the development of L2 competence over proficiency levels of a spoken learner corpus. The results show that the formula in beginner production data is likely being recalled holistically from learners’ phonological memory rather than generated online, identifiable by virtue of its fluent production in absence of any other surface structure evidence of the formula’s syntactic properties. As learners’ L2 competence increases, the formula becomes sensitive to modifications which show structural conformity at each proficiency level. The transparency between the formula’s modification and learners’ corresponding L2 surface structure realisations suggest that it is the independent development of L2 competence which integrates the formula into compositional language, and ultimately drives the SLA process forward

    2017 GREAT Day Program

    Get PDF
    SUNY Geneseo’s Eleventh Annual GREAT Day.https://knightscholar.geneseo.edu/program-2007/1011/thumbnail.jp

    Contributions to improve the technologies supporting unmanned aircraft operations

    Get PDF
    Mención Internacional en el título de doctorUnmanned Aerial Vehicles (UAVs), in their smaller versions known as drones, are becoming increasingly important in today's societies. The systems that make them up present a multitude of challenges, of which error can be considered the common denominator. The perception of the environment is measured by sensors that have errors, the models that interpret the information and/or define behaviors are approximations of the world and therefore also have errors. Explaining error allows extending the limits of deterministic models to address real-world problems. The performance of the technologies embedded in drones depends on our ability to understand, model, and control the error of the systems that integrate them, as well as new technologies that may emerge. Flight controllers integrate various subsystems that are generally dependent on other systems. One example is the guidance systems. These systems provide the engine's propulsion controller with the necessary information to accomplish a desired mission. For this purpose, the flight controller is made up of a control law for the guidance system that reacts to the information perceived by the perception and navigation systems. The error of any of the subsystems propagates through the ecosystem of the controller, so the study of each of them is essential. On the other hand, among the strategies for error control are state-space estimators, where the Kalman filter has been a great ally of engineers since its appearance in the 1960s. Kalman filters are at the heart of information fusion systems, minimizing the error covariance of the system and allowing the measured states to be filtered and estimated in the absence of observations. State Space Models (SSM) are developed based on a set of hypotheses for modeling the world. Among the assumptions are that the models of the world must be linear, Markovian, and that the error of their models must be Gaussian. In general, systems are not linear, so linearization are performed on models that are already approximations of the world. In other cases, the noise to be controlled is not Gaussian, but it is approximated to that distribution in order to be able to deal with it. On the other hand, many systems are not Markovian, i.e., their states do not depend only on the previous state, but there are other dependencies that state space models cannot handle. This thesis deals a collection of studies in which error is formulated and reduced. First, the error in a computer vision-based precision landing system is studied, then estimation and filtering problems from the deep learning approach are addressed. Finally, classification concepts with deep learning over trajectories are studied. The first case of the collection xviiistudies the consequences of error propagation in a machine vision-based precision landing system. This paper proposes a set of strategies to reduce the impact on the guidance system, and ultimately reduce the error. The next two studies approach the estimation and filtering problem from the deep learning approach, where error is a function to be minimized by learning. The last case of the collection deals with a trajectory classification problem with real data. This work completes the two main fields in deep learning, regression and classification, where the error is considered as a probability function of class membership.Los vehículos aéreos no tripulados (UAV) en sus versiones de pequeño tamaño conocidos como drones, van tomando protagonismo en las sociedades actuales. Los sistemas que los componen presentan multitud de retos entre los cuales el error se puede considerar como el denominador común. La percepción del entorno se mide mediante sensores que tienen error, los modelos que interpretan la información y/o definen comportamientos son aproximaciones del mundo y por consiguiente también presentan error. Explicar el error permite extender los límites de los modelos deterministas para abordar problemas del mundo real. El rendimiento de las tecnologías embarcadas en los drones, dependen de nuestra capacidad de comprender, modelar y controlar el error de los sistemas que los integran, así como de las nuevas tecnologías que puedan surgir. Los controladores de vuelo integran diferentes subsistemas los cuales generalmente son dependientes de otros sistemas. Un caso de esta situación son los sistemas de guiado. Estos sistemas son los encargados de proporcionar al controlador de los motores información necesaria para cumplir con una misión deseada. Para ello se componen de una ley de control de guiado que reacciona a la información percibida por los sistemas de percepción y navegación. El error de cualquiera de estos sistemas se propaga por el ecosistema del controlador siendo vital su estudio. Por otro lado, entre las estrategias para abordar el control del error se encuentran los estimadores en espacios de estados, donde el filtro de Kalman desde su aparición en los años 60, ha sido y continúa siendo un gran aliado para los ingenieros. Los filtros de Kalman son el corazón de los sistemas de fusión de información, los cuales minimizan la covarianza del error del sistema, permitiendo filtrar los estados medidos y estimarlos cuando no se tienen observaciones. Los modelos de espacios de estados se desarrollan en base a un conjunto de hipótesis para modelar el mundo. Entre las hipótesis se encuentra que los modelos del mundo han de ser lineales, markovianos y que el error de sus modelos ha de ser gaussiano. Generalmente los sistemas no son lineales por lo que se realizan linealizaciones sobre modelos que a su vez ya son aproximaciones del mundo. En otros casos el ruido que se desea controlar no es gaussiano, pero se aproxima a esta distribución para poder abordarlo. Por otro lado, multitud de sistemas no son markovianos, es decir, sus estados no solo dependen del estado anterior, sino que existen otras dependencias que los modelos de espacio de estados no son capaces de abordar. Esta tesis aborda un compendio de estudios sobre los que se formula y reduce el error. En primer lugar, se estudia el error en un sistema de aterrizaje de precisión basado en visión por computador. Después se plantean problemas de estimación y filtrado desde la aproximación del aprendizaje profundo. Por último, se estudian los conceptos de clasificación con aprendizaje profundo sobre trayectorias. El primer caso del compendio estudia las consecuencias de la propagación del error de un sistema de aterrizaje de precisión basado en visión artificial. En este trabajo se propone un conjunto de estrategias para reducir el impacto sobre el sistema de guiado, y en última instancia reducir el error. Los siguientes dos estudios abordan el problema de estimación y filtrado desde la perspectiva del aprendizaje profundo, donde el error es una función que minimizar mediante aprendizaje. El último caso del compendio aborda un problema de clasificación de trayectorias con datos reales. Con este trabajo se completan los dos campos principales en aprendizaje profundo, regresión y clasificación, donde se plantea el error como una función de probabilidad de pertenencia a una clase.I would like to thank the Ministry of Science and Innovation for granting me the funding with reference PRE2018-086793, associated to the project TEC2017-88048-C2-2-R, which provide me the opportunity to carry out all my PhD. activities, including completing an international research internship.Programa de Doctorado en Ciencia y Tecnología Informática por la Universidad Carlos III de MadridPresidente: Antonio Berlanga de Jesús.- Secretario: Daniel Arias Medina.- Vocal: Alejandro Martínez Cav

    Point Cloud Processing for Environmental Analysis in Autonomous Driving using Deep Learning

    Get PDF
    Autonomous self-driving cars need a very precise perception system of their environment, working for every conceivable scenario. Therefore, different kinds of sensor types, such as lidar scanners, are in use. This thesis contributes highly efficient algorithms for 3D object recognition to the scientific community. It provides a Deep Neural Network with specific layers and a novel loss to safely localize and estimate the orientation of objects from point clouds originating from lidar sensors. First, a single-shot 3D object detector is developed that outputs dense predictions in only one forward pass. Next, this detector is refined by fusing complementary semantic features from cameras and joint probabilistic tracking to stabilize predictions and filter outliers. The last part presents an evaluation of data from automotive-grade lidar scanners. A Generative Adversarial Network is also being developed as an alternative for target-specific artificial data generation.One of the main objectives of leading automotive companies is autonomous self-driving cars. They need a very precise perception system of their environment, working for every conceivable scenario. Therefore, different kinds of sensor types are in use. Besides cameras, lidar scanners became very important. The development in that field is significant for future applications and system integration because lidar offers a more accurate depth representation, independent from environmental illumination. Especially algorithms and machine learning approaches, including Deep Learning and Artificial Intelligence based on raw laser scanner data, are very important due to the long range and three-dimensional resolution of the measured point clouds. Consequently, a broad field of research with many challenges and unsolved tasks has been established. This thesis aims to address this deficit and contribute highly efficient algorithms for 3D object recognition to the scientific community. It provides a Deep Neural Network with specific layers and a novel loss to safely localize and estimate the orientation of objects from point clouds. First, a single shot 3D object detector is developed that outputs dense predictions in only one forward pass. Next, this detector is refined by fusing complementary semantic features from cameras and a joint probabilistic tracking to stabilize predictions and filter outliers. In the last part, a concept for deployment into an existing test vehicle focuses on the semi-automated generation of a suitable dataset. Subsequently, an evaluation of data from automotive-grade lidar scanners is presented. A Generative Adversarial Network is also being developed as an alternative for target-specific artificial data generation. Experiments on the acquired application-specific and benchmark datasets show that the presented methods compete with a variety of state-of-the-art algorithms while being trimmed down to efficiency for use in self-driving cars. Furthermore, they include an extensive set of standard evaluation metrics and results to form a solid baseline for future research.Eines der Hauptziele führender Automobilhersteller sind autonome Fahrzeuge. Sie benötigen ein sehr präzises System für die Wahrnehmung der Umgebung, dass für jedes denkbare Szenario überall auf der Welt funktioniert. Daher sind verschiedene Arten von Sensoren im Einsatz, sodass neben Kameras u. a. auch Lidar Sensoren ein wichtiger Bestandteil sind. Die Entwicklung auf diesem Gebiet ist für künftige Anwendungen von höchster Bedeutung, da Lidare eine genauere, von der Umgebungsbeleuchtung unabhängige, Tiefendarstellung bieten. Insbesondere Algorithmen und maschinelle Lernansätze wie Deep Learning, die Rohdaten über Lernzprozesse direkt verarbeiten können, sind aufgrund der großen Reichweite und der dreidimensionalen Auflösung der gemessenen Punktwolken sehr wichtig. Somit hat sich ein weites Forschungsfeld mit vielen Herausforderungen und ungelösten Problemen etabliert. Diese Arbeit zielt darauf ab, dieses Defizit zu verringern und effiziente Algorithmen zur 3D-Objekterkennung zu entwickeln. Sie stellt ein tiefes Neuronales Netzwerk mit spezifischen Schichten und einer neuartigen Fehlerfunktion zur sicheren Lokalisierung und Schätzung der Orientierung von Objekten aus Punktwolken bereit. Zunächst wird ein 3D-Detektor entwickelt, der in nur einem Vorwärtsdurchlauf aus einer Punktwolke alle Objekte detektiert. Anschließend wird dieser Detektor durch die Fusion von komplementären semantischen Merkmalen aus Kamerabildern und einem gemeinsamen probabilistischen Tracking verfeinert, um die Detektionen zu stabilisieren und Ausreißer zu filtern. Im letzten Teil wird ein Konzept für den Einsatz in einem bestehenden Testfahrzeug vorgestellt, das sich auf die halbautomatische Generierung eines geeigneten Datensatzes konzentriert. Hierbei wird eine Auswertung auf Daten von Automotive-Lidaren vorgestellt. Als Alternative zur zielgerichteten künstlichen Datengenerierung wird ein weiteres generatives Neuronales Netzwerk untersucht. Experimente mit den erzeugten anwendungsspezifischen- und Benchmark-Datensätzen zeigen, dass sich die vorgestellten Methoden mit dem Stand der Technik messen können und gleichzeitig auf Effizienz für den Einsatz in selbstfahrenden Autos optimiert sind. Darüber hinaus enthalten sie einen umfangreichen Satz an Evaluierungsmetriken und -ergebnissen, die eine solide Grundlage für die zukünftige Forschung bilden

    AI for time-resolved imaging: from fluorescence lifetime to single-pixel time of flight

    Get PDF
    Time-resolved imaging is a field of optics which measures the arrival time of light on the camera. This thesis looks at two time-resolved imaging modalities: fluorescence lifetime imaging and time-of-flight measurement for depth imaging and ranging. Both of these applications require temporal accuracy on the order of pico- or nanosecond (10−12 − 10−9s) scales. This demands special camera technology and optics that can sample light-intensity extremely quickly, much faster than an ordinary video camera. However, such detectors can be very expensive compared to regular cameras while offering lower image quality. Further, information of interest is often hidden (encoded) in the raw temporal data. Therefore, computational imaging algorithms are used to enhance, analyse and extract information from time-resolved images. "A picture is worth a thousand words". This describes a fundamental blessing and curse of image analysis: images contain extreme amounts of data. Consequently, it is very difficult to design algorithms that encompass all the possible pixel permutations and combinations that can encode this information. Fortunately, the rise of AI and machine learning (ML) allow us to instead create algorithms in a data-driven way. This thesis demonstrates the application of ML to time-resolved imaging tasks, ranging from parameter estimation in noisy data and decoding of overlapping information, through super-resolution, to inferring 3D information from 1D (temporal) data

    Managing distributed situation awareness in a team of agents

    Get PDF
    The research presented in this thesis investigates the best ways to manage Distributed Situation Awareness (DSA) for a team of agents tasked to conduct search activity with limited resources (battery life, memory use, computational power, etc.). In the first part of the thesis, an algorithm to coordinate agents (e.g., UAVs) is developed. This is based on Delaunay triangulation with the aim of supporting efficient, adaptable, scalable, and predictable search. Results from simulation and physical experiments with UAVs show good performance in terms of resources utilisation, adaptability, scalability, and predictability of the developed method in comparison with the existing fixed-pattern, pseudorandom, and hybrid methods. The second aspect of the thesis employs Bayesian Belief Networks (BBNs) to define and manage DSA based on the information obtained from the agents' search activity. Algorithms and methods were developed to describe how agents update the BBN to model the system’s DSA, predict plausible future states of the agents’ search area, handle uncertainties, manage agents’ beliefs (based on sensor differences), monitor agents’ interactions, and maintains adaptable BBN for DSA management using structural learning. The evaluation uses environment situation information obtained from agents’ sensors during search activity, and the results proved superior performance over well-known alternative methods in terms of situation prediction accuracy, uncertainty handling, and adaptability. Therefore, the thesis’s main contributions are (i) the development of a simple search planning algorithm that combines the strength of fixed-pattern and pseudorandom methods with resources utilisation, scalability, adaptability, and predictability features; (ii) a formal model of DSA using BBN that can be updated and learnt during the mission; (iii) investigation of the relationship between agents search coordination and DSA management
    corecore