63,537 research outputs found
Automated single-cell motility analysis on a chip using lensfree microscopy.
Quantitative cell motility studies are necessary for understanding biophysical processes, developing models for cell locomotion and for drug discovery. Such studies are typically performed by controlling environmental conditions around a lens-based microscope, requiring costly instruments while still remaining limited in field-of-view. Here we present a compact cell monitoring platform utilizing a wide-field (24 mm(2)) lensless holographic microscope that enables automated single-cell tracking of large populations that is compatible with a standard laboratory incubator. We used this platform to track NIH 3T3 cells on polyacrylamide gels over 20 hrs. We report that, over an order of magnitude of stiffness values, collagen IV surfaces lead to enhanced motility compared to fibronectin, in agreement with biological uses of these structural proteins. The increased throughput associated with lensfree on-chip imaging enables higher statistical significance in observed cell behavior and may facilitate rapid screening of drugs and genes that affect cell motility
Index to NASA Tech Briefs, 1975
This index contains abstracts and four indexes--subject, personal author, originating Center, and Tech Brief number--for 1975 Tech Briefs
Dial It In: Rotating RF Sensors to Enhance Radio Tomography
A radio tomographic imaging (RTI) system uses the received signal strength
(RSS) measured by RF sensors in a static wireless network to localize people in
the deployment area, without having them to carry or wear an electronic device.
This paper addresses the fact that small-scale changes in the position and
orientation of the antenna of each RF sensor can dramatically affect imaging
and localization performance of an RTI system. However, the best placement for
a sensor is unknown at the time of deployment. Improving performance in a
deployed RTI system requires the deployer to iteratively "guess-and-retest",
i.e., pick a sensor to move and then re-run a calibration experiment to
determine if the localization performance had improved or degraded. We present
an RTI system of servo-nodes, RF sensors equipped with servo motors which
autonomously "dial it in", i.e., change position and orientation to optimize
the RSS on links of the network. By doing so, the localization accuracy of the
RTI system is quickly improved, without requiring any calibration experiment
from the deployer. Experiments conducted in three indoor environments
demonstrate that the servo-nodes system reduces localization error on average
by 32% compared to a standard RTI system composed of static RF sensors.Comment: 9 page
ReCon: Revealing and Controlling PII Leaks in Mobile Network Traffic
It is well known that apps running on mobile devices extensively track and
leak users' personally identifiable information (PII); however, these users
have little visibility into PII leaked through the network traffic generated by
their devices, and have poor control over how, when and where that traffic is
sent and handled by third parties. In this paper, we present the design,
implementation, and evaluation of ReCon: a cross-platform system that reveals
PII leaks and gives users control over them without requiring any special
privileges or custom OSes. ReCon leverages machine learning to reveal potential
PII leaks by inspecting network traffic, and provides a visualization tool to
empower users with the ability to control these leaks via blocking or
substitution of PII. We evaluate ReCon's effectiveness with measurements from
controlled experiments using leaks from the 100 most popular iOS, Android, and
Windows Phone apps, and via an IRB-approved user study with 92 participants. We
show that ReCon is accurate, efficient, and identifies a wider range of PII
than previous approaches.Comment: Please use MobiSys version when referencing this work:
http://dl.acm.org/citation.cfm?id=2906392. 18 pages, recon.meddle.mob
Ontology based annotation of contextualized vital signs
Representing the kinetic state of a patient (posture, motion, and activity) during vital sign measurement is an important part of continuous monitoring applications, especially remote monitoring applications. In contextualized vital sign representation, the measurement result is presented in conjunction with salient measurement context metadata. We present an automated annotation system for vital sign measurements that uses ontologies from the Open Biomedical Ontology Foundry (OBO Foundry) to represent the patient’s kinetic state at the time of measurement. The annotation system is applied to data generated by a wearable personal status monitoring (PSM) device. We demonstrate how annotated PSM data can be queried for contextualized vital signs as well as sensor algorithm configuration parameters
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