1,682 research outputs found
Protein Profiling of Arabidopsis Roots Treated With Humic Substances: Insights Into the Metabolic and Interactome Networks
Background and Aim: Humic substances (HSs) influence the chemical and physical
properties of the soil, and are also known to affect plant physiology and nutrient uptake.
This study aimed to elucidate plant metabolic pathways and physiological processes
influenced by HS activity.
Methods: Arabidopsis roots were treated with HS for 8 h. Quantitative mass
spectrometry-based proteomics analysis of root proteins was performed using the
iTRAQ (Isobaric Tag for Relative and Absolute Quantification) technique. Out of 902
protein families identified and quantified for HS treated vs. untreated roots, 92 proteins
had different relative content. Bioinformatic tools such as STRING, KEGG, IIS and
Cytoscape were used to interpret the biological function, pathway analysis and
visualization of network amongst the identified proteins.
Results: From this analysis it was possible to evaluate that all of the identified proteins
were functionally classified into several categories, mainly redox homeostasis, response
to inorganic substances, energy metabolism, protein synthesis, cell trafficking, and
division.
Conclusion: In the present study an overview of the metabolic pathways most modified
by HS biological activity is provided. Activation of enzymes of the glycolytic pathway
and up regulation of ribosomal protein indicated a stimulation in energy metabolism and
protein synthesis. Regulation of the enzymes involved in redox homeostasis suggest a
pivotal role of reactive oxygen species in the signaling and modulation of HS-induced
responses
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