5,847 research outputs found

    An investigation into the structure of genomes within an evolution that uses embryogenesis

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    Evolutionary algorithms that use embryogenesis in the creation of individuals have several desirable qualities. Such algorithms are able to create complex, modular designs which can scale well to large problems. However, the inner workings of developmental algorithms have not been investigated as thoroughly as their direct-encoding counterparts. More precisely, it would be beneficial to look at how the rules used during embryogenesis evolve alongside the phenotypes they produced. This paper reports on such an investigation into the evolution of a rule set for the growth of an artificial neural network, and identifies several aspects that are desirable for the genomes of a developmental evolutionary algorithm

    An investigation into the structure of genomes within an evolution that uses embryogenesis

    Get PDF
    Evolutionary algorithms that use embryogenesis in the creation of individuals have several desirable qualities. Such algorithms are able to create complex, modular designs which can scale well to large problems. However, the inner workings of developmental algorithms have not been investigated as thoroughly as their direct-encoding counterparts. More precisely, it would be beneficial to look at how the rules used during embryogenesis evolve alongside the phenotypes they produced. This paper reports on such an investigation into the evolution of a rule set for the growth of an artificial neural network, and identifies several aspects that are desirable for the genomes of a developmental evolutionary algorithm

    Programmed cell death and genetic stability in conifer embryogenesis

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    Somatic embryogenesis, the generation of embryos from somatic cells, is a valuable tool for studying embryology. In addition, somatic embryos can be used for large-scale vegetative propagation, an application of great interest for forestry. A critical event during early embryo differentiation in conifers is the apical basal polarization, which proceeds through the establishment of two embryonic parts: the proliferating embryonal mass and the terminally differentiated suspensor. The development of both parts is strictly coordinated and imbalance causes embryonic defects. The suspensor cells are eliminated by programmed cell death (PCD). In animals, caspase family proteases are the main executioners of PCD. In this work we have used synthetic peptide substrates containing caspase recognition sites and corresponding specific inhibitors to analyse the role of caspase-like activity during early embryo differentiation in Norway spruce (Picea abies L. Karst.). We found that VEIDase is the principal caspase-like activity. This activity is localized specifically in suspensor cells, and its inhibition prevents normal embryo development by blocking the suspensor differentiation. The in vitro VEIDase activity was shown to be highly sensitive to pH, ionic strength, temperature and zinc concentration. In vivo studies with Zinquin, a zinc-specific fluorescent probe, revealed a high accumulation of intracellular free zinc in the embryonal masses and an abrupt decrease in the suspensor. Increased zinc concentration in the culture medium suppresses terminal differentiation and PCD of the suspensor. In accordance, exposure of early embryos to TPEN, a zinc-specific chelator, induces ectopic cell death affecting embryonal masses. This establishes zinc as an important factor affecting cell fate specification during plant embryogenesis. Before somatic embryos can be accepted for clonal propagation it is important to show that the regenerated plants have similar growth to that of seedlings and are genetically uniform. The genetic integrity during zygotic and somatic embryogenesis in Norway spruce and Scots pine (Pinus sylvestris L.) was investigated by comparing the stability of variable nuclear microsatellite loci. The stability varied significantly among families in both species during somatic embryogenesis. Scots pine families showing low genetic stability during establishment of embryogenic cultures had a higher embryogenic potential than those that were genetically more stable. In contrast, embryo development was suppressed in genetically unstable families. The stability of microsatellites was in general higher in zygotic embryos than in somatic embryos. No deviation in growth was observed in somatic embryo plants of Norway spruce carrying mutated microsatellites

    Barley (Hordeum vulgare L.) Improvement Past, Present and Future

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    Barley has been cultivated for more than 10,000 years. Barley improvement studies always have the privilege of the breeders and scientists. This review is expected to provide a resource for researchers interested in barley improvement research in terms of mutation breeding, tissue culture, gene transfers, gene editing, molecular markers, transposons, epigenetic, genomic studies and system biology. We aimed to discuss some important and/or recent studies and improvements about barley for understanding the factors responsible for converting barley plants into the superior cereals, which occurred through gene transfers, gene editing and molecular breeding, which is important and could help us enhance the current pool of cultivated barley species to provide enough material for the future
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