The impact of agricultural activities on water quality: a case for collaborative catchment-scale management using integrated wireless sensor networks

The challenge of improving water quality is a growing global concern, typified by the European Commission Water Framework Directive and the United States Clean Water Act. The main drivers of poor water quality are economics, poor water management, agricultural practices and urban development. This paper reviews the extensive role of non-point sources, in particular the outdated agricultural practices, with respect to nutrient and contaminant contributions. Water quality monitoring (WQM) is currently undertaken through a number of data acquisition methods from grab sampling to satellite based remote sensing of water bodies. Based on the surveyed sampling methods and their numerous limitations, it is proposed that wireless sensor networks (WSNs), despite their own limitations, are still very attractive and effective for real-time spatio-temporal data collection for WQM applications. WSNs have been employed for WQM of surface and ground water and catchments, and have been fundamental in advancing the knowledge of contaminants trends through their high resolution observations. However, these applications have yet to explore the implementation and impact of this technology for management and control decisions, to minimize and prevent individual stakeholder’s contributions, in an autonomous and dynamic manner. Here, the potential of WSN-controlled agricultural activities and different environmental compartments for integrated water quality management is presented and limitations of WSN in agriculture and WQM are identified. Finally, a case for collaborative networks at catchment scale is proposed for enabling cooperation among individually networked activities/stakeholders (farming activities, water bodies) for integrated water quality monitoring, control and management

The Ecology and Sociology of the Mission-Aransas Estuary : An Estuarine and Watershed Profile

watershed profileThe Mission-Aransas National Estuarine Research Reserve (NERR) is one of 28 national estuarine reserves created to promote the responsible use and management of the nation's estuaries through a program combining scientific research, education, and stewardship. The purpose of this document is to provide researchers and resource managers with an adequate basis of knowledge to further development of scientific studies and applied management investigations. This document describes the different physical ecosystem components, ecological processes, habitats, and watersheds of the Reserve. The Mission-Aransas NERR is a complex of wetland, terrestrial, and marine environments. The land is primarily coastal prairie with unique oak motte habitats. The wetlands include riparian habitat, and freshwater and salt water marshes. Within the water areas, the bays are large, open, and include extensive wind tidal flats, seagrass meadows, mangroves, and oyster reefs. This site profile describes each habitat by their location, type, distribution, abundance, current status and trends, issues of concerns, and future research plans. Research within the Mission-Aransas NERR seeks to improve the understanding of the Texas coastal zone ecosystems structure and function. Current research includes: nutrient loading and transformation, estimates of community metabolism, water quality monitoring, freshwater inflow, climate change and fishery habitat. Harmful algal blooms, zooplankton, coliform bacteria, submerged aquatic vegetation, and marsh grass are monitored through the System- Wide Monitoring Program (SWMP). This document also describes the climate, hydrography and oceanography, geology, water quality, and endangered species within the Mission-AransasUniversity of Texas Marine Science InstituteMarine Scienc

Water Temperature Protocol

The purpose of this resource is to measure the temperature of a water sample. Students use an alcohol-filled thermometer or meter to measure the temperature of water. The meter requires calibration before use; the accuracy of the thermometer needs to be checked before use. Educational levels: Primary elementary, Intermediate elementary, Middle school, High school

Guiding the development of a controlled ecological life support system

The workshop is reported which was held to establish guidelines for future development of ecological support systems, and to develop a group of researchers who understand the interdisciplinary requirements of the overall program

Genome Editing Method for the Anaerobic Magnetotactic Bacterium Desulfovibrio magneticus RS-1.

Magnetosomes are complex bacterial organelles that serve as model systems for studying bacterial cell biology, biomineralization, and global iron cycling. Magnetosome biogenesis is primarily studied in two closely related Alphaproteobacteria of the genus Magnetospirillum that form cubooctahedral-shaped magnetite crystals within a lipid membrane. However, chemically and structurally distinct magnetic particles have been found in physiologically and phylogenetically diverse bacteria. Due to a lack of molecular genetic tools, the mechanistic diversity of magnetosome formation remains poorly understood. Desulfovibrio magneticus RS-1 is an anaerobic sulfate-reducing deltaproteobacterium that forms bullet-shaped magnetite crystals. A recent forward genetic screen identified 10 genes in the conserved magnetosome gene island of D. magneticus that are essential for its magnetic phenotype. However, this screen likely missed mutants with defects in crystal size, shape, and arrangement. Reverse genetics to target the remaining putative magnetosome genes using standard genetic methods of suicide vector integration have not been feasible due to the low transconjugation efficiency. Here, we present a reverse genetic method for targeted mutagenesis in D. magneticus using a replicative plasmid. To test this method, we generated a mutant resistant to 5-fluorouracil by making a markerless deletion of the upp gene that encodes uracil phosphoribosyltransferase. We also used this method for targeted marker exchange mutagenesis by replacing kupM, a gene identified in our previous screen as a magnetosome formation factor, with a streptomycin resistance cassette. Overall, our results show that targeted mutagenesis using a replicative plasmid is effective in D. magneticus and may also be applied to other genetically recalcitrant bacteria.IMPORTANCE Magnetotactic bacteria (MTB) are a group of organisms that form intracellular nanometer-scale magnetic crystals though a complex process involving lipid and protein scaffolds. These magnetic crystals and their lipid membranes, termed magnetosomes, are model systems for studying bacterial cell biology and biomineralization and are potential platforms for biotechnological applications. Due to a lack of genetic tools and unculturable representatives, the mechanisms of magnetosome formation in phylogenetically deeply branching MTB remain unknown. These MTB contain elongated bullet-/tooth-shaped magnetite and greigite crystals that likely form in a manner distinct from that of the cubooctahedral-shaped magnetite crystals of the genetically tractable MTB within the Alphaproteobacteria Here, we present a method for genome editing in Desulfovibrio magneticus RS-1, a cultured representative of the deeply branching MTB of the class Deltaproteobacteria This marks a crucial step in developing D. magneticus as a model for studying diverse mechanisms of magnetic particle formation by MTB