6,812 research outputs found
The State of the Art in Deep Learning Applications, Challenges, and Future Prospects::A Comprehensive Review of Flood Forecasting and Management
Floods are a devastating natural calamity that may seriously harm both infrastructure and people. Accurate flood forecasts and control are essential to lessen these effects and safeguard populations. By utilizing its capacity to handle massive amounts of data and provide accurate forecasts, deep learning has emerged as a potent tool for improving flood prediction and control. The current state of deep learning applications in flood forecasting and management is thoroughly reviewed in this work. The review discusses a variety of subjects, such as the data sources utilized, the deep learning models used, and the assessment measures adopted to judge their efficacy. It assesses current approaches critically and points out their advantages and disadvantages. The article also examines challenges with data accessibility, the interpretability of deep learning models, and ethical considerations in flood prediction. The report also describes potential directions for deep-learning research to enhance flood predictions and control. Incorporating uncertainty estimates into forecasts, integrating many data sources, developing hybrid models that mix deep learning with other methodologies, and enhancing the interpretability of deep learning models are a few of these. These research goals can help deep learning models become more precise and effective, which will result in better flood control plans and forecasts. Overall, this review is a useful resource for academics and professionals working on the topic of flood forecasting and management. By reviewing the current state of the art, emphasizing difficulties, and outlining potential areas for future study, it lays a solid basis. Communities may better prepare for and lessen the destructive effects of floods by implementing cutting-edge deep learning algorithms, thereby protecting people and infrastructure
Anuário científico da Escola Superior de Tecnologia da Saúde de Lisboa - 2021
É com grande prazer que apresentamos a mais recente edição (a 11.ª) do Anuário Científico da Escola Superior de Tecnologia da Saúde de Lisboa. Como instituição de ensino superior, temos o compromisso de promover e incentivar a pesquisa científica em todas as áreas do conhecimento que contemplam a nossa missão. Esta publicação tem como objetivo divulgar toda a produção científica desenvolvida pelos Professores, Investigadores, Estudantes e Pessoal não Docente da ESTeSL durante 2021. Este Anuário é, assim, o reflexo do trabalho árduo e dedicado da nossa comunidade, que se empenhou na produção de conteúdo científico de elevada qualidade e partilhada com a Sociedade na forma de livros, capítulos de livros, artigos publicados em revistas nacionais e internacionais, resumos de comunicações orais e pósteres, bem como resultado dos trabalhos de 1º e 2º ciclo. Com isto, o conteúdo desta publicação abrange uma ampla variedade de tópicos, desde temas mais fundamentais até estudos de aplicação prática em contextos específicos de Saúde, refletindo desta forma a pluralidade e diversidade de áreas que definem, e tornam única, a ESTeSL. Acreditamos que a investigação e pesquisa científica é um eixo fundamental para o desenvolvimento da sociedade e é por isso que incentivamos os nossos estudantes a envolverem-se em atividades de pesquisa e prática baseada na evidência desde o início dos seus estudos na ESTeSL. Esta publicação é um exemplo do sucesso desses esforços, sendo a maior de sempre, o que faz com que estejamos muito orgulhosos em partilhar os resultados e descobertas dos nossos investigadores com a comunidade científica e o público em geral. Esperamos que este Anuário inspire e motive outros estudantes, profissionais de saúde, professores e outros colaboradores a continuarem a explorar novas ideias e contribuir para o avanço da ciência e da tecnologia no corpo de conhecimento próprio das áreas que compõe a ESTeSL. Agradecemos a todos os envolvidos na produção deste anuário e desejamos uma leitura inspiradora e agradável.info:eu-repo/semantics/publishedVersio
Estudo da remodelagem reversa miocárdica através da análise proteómica do miocárdio e do líquido pericárdico
Valve replacement remains as the standard therapeutic option for aortic
stenosis patients, aiming at abolishing pressure overload and triggering
myocardial reverse remodeling. However, despite the instant hemodynamic
benefit, not all patients show complete regression of myocardial hypertrophy,
being at higher risk for adverse outcomes, such as heart failure. The current
comprehension of the biological mechanisms underlying an incomplete reverse
remodeling is far from complete. Furthermore, definitive prognostic tools and
ancillary therapies to improve the outcome of the patients undergoing valve
replacement are missing. To help abridge these gaps, a combined myocardial
(phospho)proteomics and pericardial fluid proteomics approach was followed,
taking advantage of human biopsies and pericardial fluid collected during
surgery and whose origin anticipated a wealth of molecular information
contained therein.
From over 1800 and 750 proteins identified, respectively, in the myocardium
and in the pericardial fluid of aortic stenosis patients, a total of 90 dysregulated
proteins were detected. Gene annotation and pathway enrichment analyses,
together with discriminant analysis, are compatible with a scenario of increased
pro-hypertrophic gene expression and protein synthesis, defective ubiquitinproteasome system activity, proclivity to cell death (potentially fed by
complement activity and other extrinsic factors, such as death receptor
activators), acute-phase response, immune system activation and fibrosis.
Specific validation of some targets through immunoblot techniques and
correlation with clinical data pointed to complement C3 β chain, Muscle Ring
Finger protein 1 (MuRF1) and the dual-specificity Tyr-phosphorylation
regulated kinase 1A (DYRK1A) as potential markers of an incomplete
response. In addition, kinase prediction from phosphoproteome data suggests
that the modulation of casein kinase 2, the family of IκB kinases, glycogen
synthase kinase 3 and DYRK1A may help improve the outcome of patients
undergoing valve replacement. Particularly, functional studies with DYRK1A+/-
cardiomyocytes show that this kinase may be an important target to treat
cardiac dysfunction, provided that mutant cells presented a different response
to stretch and reduced ability to develop force (active tension).
This study opens many avenues in post-aortic valve replacement reverse
remodeling research. In the future, gain-of-function and/or loss-of-function
studies with isolated cardiomyocytes or with animal models of aortic bandingdebanding will help disclose the efficacy of targeting the surrogate therapeutic
targets. Besides, clinical studies in larger cohorts will bring definitive proof of
complement C3, MuRF1 and DYRK1A prognostic value.A substituição da válvula aórtica continua a ser a opção terapêutica de
referência para doentes com estenose aórtica e visa a eliminação da
sobrecarga de pressão, desencadeando a remodelagem reversa miocárdica.
Contudo, apesar do benefício hemodinâmico imediato, nem todos os pacientes
apresentam regressão completa da hipertrofia do miocárdio, ficando com maior
risco de eventos adversos, como a insuficiência cardíaca. Atualmente, os
mecanismos biológicos subjacentes a uma remodelagem reversa incompleta
ainda não são claros. Além disso, não dispomos de ferramentas de
prognóstico definitivos nem de terapias auxiliares para melhorar a condição
dos pacientes indicados para substituição da válvula. Para ajudar a resolver
estas lacunas, uma abordagem combinada de (fosfo)proteómica e proteómica
para a caracterização, respetivamente, do miocárdio e do líquido pericárdico
foi seguida, tomando partido de biópsias e líquidos pericárdicos recolhidos em
ambiente cirúrgico.
Das mais de 1800 e 750 proteínas identificadas, respetivamente, no miocárdio
e no líquido pericárdico dos pacientes com estenose aórtica, um total de 90
proteínas desreguladas foram detetadas. As análises de anotação de genes,
de enriquecimento de vias celulares e discriminativa corroboram um cenário de
aumento da expressão de genes pro-hipertróficos e de síntese proteica, um
sistema ubiquitina-proteassoma ineficiente, uma tendência para morte celular
(potencialmente acelerada pela atividade do complemento e por outros fatores
extrínsecos que ativam death receptors), com ativação da resposta de fase
aguda e do sistema imune, assim como da fibrose.
A validação de alguns alvos específicos através de immunoblot e correlação
com dados clínicos apontou para a cadeia β do complemento C3, a Muscle
Ring Finger protein 1 (MuRF1) e a dual-specificity Tyr-phosphoylation
regulated kinase 1A (DYRK1A) como potenciais marcadores de uma resposta
incompleta. Por outro lado, a predição de cinases a partir do fosfoproteoma,
sugere que a modulação da caseína cinase 2, a família de cinases do IκB, a
glicogénio sintase cinase 3 e da DYRK1A pode ajudar a melhorar a condição
dos pacientes indicados para intervenção. Em particular, a avaliação funcional
de cardiomiócitos DYRK1A+/- mostraram que esta cinase pode ser um alvo
importante para tratar a disfunção cardíaca, uma vez que os miócitos mutantes
responderam de forma diferente ao estiramento e mostraram uma menor
capacidade para desenvolver força (tensão ativa).
Este estudo levanta várias hipóteses na investigação da remodelagem reversa.
No futuro, estudos de ganho e/ou perda de função realizados em
cardiomiócitos isolados ou em modelos animais de banding-debanding da
aorta ajudarão a testar a eficácia de modular os potenciais alvos terapêuticos
encontrados. Além disso, estudos clínicos em coortes de maior dimensão
trarão conclusões definitivas quanto ao valor de prognóstico do complemento
C3, MuRF1 e DYRK1A.Programa Doutoral em Biomedicin
Forested buffers in agricultural landscapes : mitigation effects on stream–riparian meta-ecosystems
Stream–riparian meta-ecosystems are strongly connected through exchanges of
energy, material and organisms. Land use can disrupt ecological connectivity by
affecting community composition directly and/or indirectly by altering the instream
and riparian habitats that support biological structure and function. Although
forested riparian buffers are increasingly used as a management intervention, our
understanding of their effects on the functioning of stream–riparian metaecosystems
is limited. This study assessed patterns in the longitudinal and lateral
profiles of streams in modified landscapes across Europe and Sweden using a pairedreach
approach, with upstream unbuffered reaches lacking woody riparian
vegetation and with downstream reaches having well-developed forested buffers.
The presence of buffers was positively associated with stream ecological status as
well as important attributes, which included instream shading and the provision of
suitable habitats for instream and riparian communities, thus supporting more
aquatic insects (especially EPT taxa). Emergence of aquatic insects is particularly
important because they mediate reciprocal flows of subsidies into terrestrial systems.
Results of fatty acid analysis and prey DNA from spiders further supported the
importance of buffers in providing more aquatic-derived quality food (i.e. essential
fatty acids) for riparian spiders. Findings presented in this thesis show that buffers
contribute to the strengthening of cross-ecosystem connectivity and have the
potential to affect a wide range of consumers in modified landscapes
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Into the Multiverse: Methods for Studying Developmental Neuroscience
One major challenge in developmental neuroscience research is the sheer number of choices researchers face when addressing even a single research question. Even once data collection is complete, the journey from raw data to interpretation of findings may depend on numerous decisions. To address this issue, this dissertation explores “multiverse” analysis techniques for following many analytical paths at once in the same dataset.
In chapter 1, multiverses are used to examine which analyses of age-related change in amygdala-medial prefrontal cortex circuitry are robust versus sensitive to researcher decisions. Chapter 2 uses multiverse analysis to identify optimal solutions for mitigating breathing-induced artifacts in resting-state functional magnetic resonance imaging data. Chapter 3 uses a variety of model specifications to characterize simultaneous reward learning strategies in youth contingent on both visual task cues and spatial-motor information.
Despite varied approaches and goals, each of the three studies highlight the benefits of conducting multiple parallel analyses for both addressing questions in developmental neuroscience and deepening understanding of the methods used to address them
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Co-design As Healing: Exploring The Experiences Of Participants Facing Mental Health Problems
This thesis is an exploration of the healing role of co-design in mental health. Although co-design projects conducted within mental health settings are rising, existing literature tends to focus on the object of design and its outcomes while the experiences of participants per se remain largely unexplored. The guiding research question of this study is not how we design things that improve mental health, but how co-designing, as an act, might do so.
The thesis presents two projects that were organized in collaboration with the mental health charity Islington Mind and the Psychosis Therapy Project (PTP) in London.
The project at Islington Mind used a structured design process inviting participants to design for wellbeing. A case study analysis provides insights on how participants were impacted, summarizing key challenges and opportunities.
The design at PTP worked towards creating a collective brief in an emergent fashion, finally culminating in a board game. The experiences of participants were explored through Interpretative Phenomenological Analysis (IPA), using semi-structured interview data. The analysis served to identify key themes characterising the experience of co-design such as contributing, connecting, thinking and intentioning. In addition, a mixed-methods analysis of questionnaires and interview data exploring participants' wellbeing, showed that all participants who engaged fairly consistently in the project improved after the project ended, although some participants' scores returned to baseline six months later.
Reflecting on both projects, an approach to facilitation within mental health is outlined, detailing how the dimensions of weaving and layered participation, nurturing mattering and facilitating attitudes interlace. This contribution raises awareness of tacit dimensions in the practice of facilitation, articulating the nuances of how to encourage and sustain meaningful and ethical engagement and offering insights into a range of tools. It highlights the importance of remaining reflexive in relation to attitudes and emotions and discusses practical methodological and ethical challenges and ways to resolve them which can be of benefit to researchers embarking on a similar journey.
The thesis also offers detailed insights on how methodologies from different fields were integrated into a whole, arguing for transparency and reflexivity about epistemological assumptions, and how underlying paradigms shift in an interdisciplinary context.
Based on the overall findings, the thesis makes a case for considering design as healing (or a designerly way of healing), highlighting implications at a systems, social and individual level. It makes an original contribution to our understanding of design, highlighting its healing character, and proposes a new way to support mental health. The participants in this study not only had increased their own wellbeing through co-designing, but were also empowered and contributed towards healing the world. Hence, the thesis argues for a unique, holistic perspective of design and mental health, recognizing the interconnectedness of the individual, social and systemic dimensions of the healing processes that are ignited
Annals [...].
Pedometrics: innovation in tropics; Legacy data: how turn it useful?; Advances in soil sensing; Pedometric guidelines to systematic soil surveys.Evento online. Coordenado por: Waldir de Carvalho Junior, Helena Saraiva Koenow Pinheiro, Ricardo Simão Diniz Dalmolin
Cultivating Agrobiodiversity in the U.S.: Barriers and Bridges at Multiple Scales
The diversity of crops grown in the United States (U.S.) is declining, causing agricultural landscapes to become more and more simplified. This trend is concerning for the loss of important plant, insect, and animal species, as well as the pollution and degradation of our environment. Through three separate but related studies, this dissertation addresses the need to increase the diversity of these agricultural landscapes in the U.S., particularly through diversifying the type and number of crops grown. The first study uses multiple, openly accessible datasets related to agricultural land use and policies to document and visualize change over recent decades. Through this, I show that U.S. agriculture has gradually become more specialized in the crops grown, crop production is heavily concentrated in certain areas, and crop diversity is continuing to decline. Meanwhile, federal agricultural policy, while having become more influential over how U.S. agriculture operates, incentivizes this specialization. The second study uses nonlinear statistical modeling to identify and compare social, political, and ecological factors that best predict crop diversity across nine regions in the U.S. Factors of climate, prior land use, and farm inputs best predict diversity across regions, but regions show key differences in how factors are important, indicating that patterns at the regional scale constrain and enable further diversification. Finally, the third study relied on interviews with farmers and key informants in southern Idaho’s Magic Valley – a cluster of eight counties that is known to be agriculturally diverse. Interviews gauge what farmers are currently doing to manage crop diversity (the present) and how they imagine alternative landscapes (the imaginary). We found that farmers in the Magic Valley manage current diversity mainly through cover cropping and diverse crop rotations, but daily struggles and political barriers make experimenting with and imagining alternative landscapes difficult and unlikely to occur. Together, these three studies provide an integrated view of how and why U.S. agriculture landscapes simplify or diversify, as well as the barriers and bridges such pathways of diversification
The Future of Work and Digital Skills
The theme for the events was "The Future of Work and Digital Skills". The 4IR caused a
hollowing out of middle-income jobs (Frey & Osborne, 2017) but COVID-19 exposed the digital gap as survival depended mainly on digital infrastructure and connectivity. Almost overnight, organizations that had not invested in a digital strategy suddenly realized the need for such a strategy and the associated digital skills. The effects have been profound for those who struggled to adapt, while those who stepped up have reaped quite the reward.Therefore, there are no longer certainties about what the world will look like in a few years from now. However, there are certain ways to anticipate the changes that are occurring and plan on how to continually adapt to an increasingly changing world. Certain jobs will soon be lost and will not come back; other new jobs will however be created. Using data science and other predictive sciences, it is possible to anticipate, to the extent possible, the rate at which certain jobs will be replaced and new jobs created in different industries. Accordingly, the collocated events sought to bring together government, international organizations, academia, industry, organized labour and civil society to deliberate on how these changes are occurring in South Africa, how fast they are occurring and what needs to change in order to prepare society for the changes.Deutsche Gesellschaft für Internationale Zusammenarbeit (GIZ)
British High Commission (BHC)School of Computin
Investigating novel aspects of the blood-brain barrier using high resolution electron microscopy
Doctor ScientiaeThe blood-brain barrier (BBB) is a restrictive interface located between the blood
circulation and the central nervous system (CNS), regulating the homeostatic
environment of the neuronal milieu, by controlling the permeability of the
cerebrovasculature. Currently, we cannot fully comprehend the regulatory features
and the complexity of BBB morphology to allow for intervention clinically. The
thesis consists of four publications. The methodology paper proposes a novel
experimental design to visualize the morphological architecture of immortalized
mouse brain endothelial cell lines (bEnd3/bEnd5). The brain endothelial cells
(BECs) were grown on cellulose matrices and fixed in 2.5 % glutaraldehyde in
preparation for visualization of the paracellular (PC) spaces between adjacent
BECs, employing high-resolution electron microscopy (HREM), with vested
interest in the morphological profile of the developing BEC. The second
publication addresses and reports on the nanosized detail of BEC monolayer
morphology utilizing high-resolution scanning electron microscopy (HR-SEM)
and published the first descriptions of the extrusion of a basement membrane from
developing in vitro BECs. Moreover, we categorized and discussed two types of
nanotubule (NT) development specific for the establishment of the BEC
monolayers. NTs can occur via nanovesicle extrusion onto the BEC membrane
surfaces, which fuse, forming tunneling NTs (TUNTs) between adjacent BECs.
Furthermore, cytoplasmic extensions of BEC membrane leading edges give rise to
tethering NT (TENTs), which result in overlapping regions across the PC spaces,
resulting in PC occlusion. BEC NT communication is illuminated in a third
publication utilizing immunofluorescence microscopy, which reports on the
molecular, cytoskeletal elements governing NT formation. This study shows, for
the first time, f-actin and α-tubulin cytoskeletal proteins extending between the
soma of the cells and NT cytoskeletal structures within an in vitro BBB model.
Thereafter, the effects depolymerizing agents, Cytochalasin D and Nocodazole,
were investigated on f-actin and α-tubulin cytoskeletal protein generation,functionality of NT morphology, cell division and permeability. For the first time,
we show that f-actin possesses an additional function, key to tight junction, plaque
protein organization. Moreover, it facilitates TENT formation, essential for
cytoplasmic projection across PC spaces. Conversely, α-tubulin facilitates known
functions: (i) transportation, (ii) cytokinesis, (iii) cellular division, and (iv)
possesses a novel function as the molecular cytoskeletal backbone of TENTs,
which facilitates BBB impermeability. A critical review evaluates past literature,
in light of the current findings emanating from this study. The review critiques the
concept of BEC cilia, which have been reported in the literature, comprised of
tubulin and actin, but at low-resolution. In the light of our novel observations,
nowhere in transmission electron microscopy do we observe cilia on the BECs,
we postulate that NTs have been misnamed and mischaracterized as cilia. The
thesis endeavors to elucidate the complexity of BEC nanostructures by examining
the emerging role of the nanoscopic landscape of BBB development and the
changing nature of BEC morphology, NT formation and associated
cytoarchitectural underpinnings governing NT morphology. The research study
attempts to, with a view to create new avenues for treating brain pathology,
revolutionize our interpretation of barrier-genesis on a nanoscale
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