PyZebrascope: An Open-Source Platform for Brain-Wide Neural Activity Imaging in Zebrafish

Understanding how neurons interact across the brain to control animal behaviors is one of the central goals in neuroscience. Recent developments in fluorescent microscopy and genetically-encoded calcium indicators led to the establishment of whole-brain imaging methods in zebrafish, which record neural activity across a brain-wide volume with single-cell resolution. Pioneering studies of whole-brain imaging used custom light-sheet microscopes, and their operation relied on commercially developed and maintained software not available globally. Hence it has been challenging to disseminate and develop the technology in the research community. Here, we present PyZebrascope, an open-source Python platform designed for neural activity imaging in zebrafish using light-sheet microscopy. PyZebrascope has intuitive user interfaces and supports essential features for whole-brain imaging, such as two orthogonal excitation beams and eye damage prevention. Its camera module can handle image data throughput of up to 800 MB/s from camera acquisition to file writing while maintaining stable CPU and memory usage. Its modular architecture allows the inclusion of advanced algorithms for microscope control and image processing. As a proof of concept, we implemented a novel automatic algorithm for maximizing the image resolution in the brain by precisely aligning the excitation beams to the image focal plane. PyZebrascope enables whole-brain neural activity imaging in fish behaving in a virtual reality environment. Thus, PyZebrascope will help disseminate and develop light-sheet microscopy techniques in the neuroscience community and advance our understanding of whole-brain neural dynamics during animal behaviors.Peer Reviewe

Deep Learning based Virtual Point Tracking for Real-Time Target-less Dynamic Displacement Measurement in Railway Applications

In the application of computer-vision based displacement measurement, an optical target is usually required to prove the reference. In the case that the optical target cannot be attached to the measuring objective, edge detection, feature matching and template matching are the most common approaches in target-less photogrammetry. However, their performance significantly relies on parameter settings. This becomes problematic in dynamic scenes where complicated background texture exists and varies over time. To tackle this issue, we propose virtual point tracking for real-time target-less dynamic displacement measurement, incorporating deep learning techniques and domain knowledge. Our approach consists of three steps: 1) automatic calibration for detection of region of interest; 2) virtual point detection for each video frame using deep convolutional neural network; 3) domain-knowledge based rule engine for point tracking in adjacent frames. The proposed approach can be executed on an edge computer in a real-time manner (i.e. over 30 frames per second). We demonstrate our approach for a railway application, where the lateral displacement of the wheel on the rail is measured during operation. We also implement an algorithm using template matching and line detection as the baseline for comparison. The numerical experiments have been performed to evaluate the performance and the latency of our approach in the harsh railway environment with noisy and varying backgrounds

Earth resources technology satellite operations control center and data processing facility. Book 2 - Systems studies Final report

Systems analysis for ERTS NASA Data Processing Facility system and subsystem

NASA SBIR abstracts of 1991 phase 1 projects

The objectives of 301 projects placed under contract by the Small Business Innovation Research (SBIR) program of the National Aeronautics and Space Administration (NASA) are described. These projects were selected competitively from among proposals submitted to NASA in response to the 1991 SBIR Program Solicitation. The basic document consists of edited, non-proprietary abstracts of the winning proposals submitted by small businesses. The abstracts are presented under the 15 technical topics within which Phase 1 proposals were solicited. Each project was assigned a sequential identifying number from 001 to 301, in order of its appearance in the body of the report. Appendixes to provide additional information about the SBIR program and permit cross-reference of the 1991 Phase 1 projects by company name, location by state, principal investigator, NASA Field Center responsible for management of each project, and NASA contract number are included

PyZebraScope: an open-source platform for brain-wide neural activity imaging in zebrafish

Understanding how neurons interact across the brain to control animal behaviors is one of the central goals in neuroscience. Recent developments in fluorescent microscopy and genetically-encoded calcium indicators led to the establishment of whole-brain imaging methods in zebrafish, which records neural activity across a brain-wide volume with single-cell resolution. Pioneering studies of whole-brain imaging used custom light-sheet microscopes, and their operation relied on commercially developed and maintained software that is not available globally. Hence it has been challenging to disseminate and develop the technology in the research community. Here, we present PyZebrascope, an open-source Python platform designed for neural activity imaging in zebrafish using light-sheet microscopy. PyZebrascope has intuitive user interfaces and implements essential features for whole-brain imaging, such as two orthogonal excitation beams and eye damage prevention. Its modular architecture allows the inclusion of advanced algorithms for microscope control and image processing. As a proof of concept, we implemented an automatic algorithm for maximizing the image resolution in the brain by precisely aligning the excitation beams to the image focal plane. PyZebrascope enables whole-brain neural activity imaging in fish behaving in a virtual reality environment with a stable high data throughput and low CPU and memory consumption. Thus, PyZebrascope will help disseminate and develop light-sheet microscopy techniques in the neuroscience community and advance our understanding of whole-brain neural dynamics during animal behaviors

UAV or Drones for Remote Sensing Applications in GPS/GNSS Enabled and GPS/GNSS Denied Environments

The design of novel UAV systems and the use of UAV platforms integrated with robotic sensing and imaging techniques, as well as the development of processing workflows and the capacity of ultra-high temporal and spatial resolution data, have enabled a rapid uptake of UAVs and drones across several industries and application domains.This book provides a forum for high-quality peer-reviewed papers that broaden awareness and understanding of single- and multiple-UAV developments for remote sensing applications, and associated developments in sensor technology, data processing and communications, and UAV system design and sensing capabilities in GPS-enabled and, more broadly, Global Navigation Satellite System (GNSS)-enabled and GPS/GNSS-denied environments.Contributions include:UAV-based photogrammetry, laser scanning, multispectral imaging, hyperspectral imaging, and thermal imaging;UAV sensor applications; spatial ecology; pest detection; reef; forestry; volcanology; precision agriculture wildlife species tracking; search and rescue; target tracking; atmosphere monitoring; chemical, biological, and natural disaster phenomena; fire prevention, flood prevention; volcanic monitoring; pollution monitoring; microclimates; and land use;Wildlife and target detection and recognition from UAV imagery using deep learning and machine learning techniques;UAV-based change detection