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Aligning scan acquisition circles in optical coherence tomography images of the retinal nerve fibre layer
Optical coherence tomography (OCT) is widely used in the assessment of retinal nerve fibre layer thickness (RNFLT) in glaucoma. Images are typically acquired with a circular scan around the optic nerve head. Accurate registration of OCT scans is essential for measurement reproducibility and longitudinal examination. This study developed and evaluated a special image registration algorithm to align the location of the OCT scan circles to the vessel features in the retina using probabilistic modelling that was optimised by an expectation-maximization algorithm. Evaluation of the method on 18 patients undergoing large numbers of scans indicated improved data acquisition and better reproducibility of measured RNFLT when scanning circles were closely matched. The proposed method enables clinicians to consider the RNFLT measurement and its scan circle location on the retina in tandem, reducing RNFLT measurement variability and assisting detection of real change of RNFLT in the longitudinal assessment of glaucoma
Glycosylases and AP-cleaving enzymes as a general tool for probe-directed cleavage of ssDNA targets
The current arsenal of molecular tools for site-directed cleavage of single-stranded DNA (ssDNA) is limited. Here, we describe a method for targeted DNA cleavage that requires only the presence of an A nucleotide at the target position. The procedure involves hybridization of a complementary oligonucleotide probe to the target sequence. The probe is designed to create a deliberate G:A mismatch at the desired position of cleavage. The DNA repair enzyme MutY glycosylase recognizes the mismatch structure and selectively removes the mispaired A from the duplex to create an abasic site in the target strand. Addition of an AP-endonuclease, such as Endonuclease IV, subsequently cleaves the backbone dividing the DNA strand into two fragments. With an appropriate choice of an AP-cleaving enzyme, the 3ā²- and 5ā²-ends of the cleaved DNA are suitable to take part in subsequent enzymatic reactions such as priming for polymerization or joining by DNA ligation. We define suitable standard reaction conditions for glycosylase/AP-cleaving enzyme (G/AP) cleavage, and demonstrate the use of the method in an improved scheme for in situ detection using target-primed rolling-circle amplification of padlock probes
Three-Dimensional Spectral-Domain Optical Coherence Tomography Data Analysis for Glaucoma Detection
Purpose: To develop a new three-dimensional (3D) spectral-domain optical coherence tomography (SD-OCT) data analysis method using a machine learning technique based on variable-size super pixel segmentation that efficiently utilizes full 3D dataset to improve the discrimination between early glaucomatous and healthy eyes. Methods: 192 eyes of 96 subjects (44 healthy, 59 glaucoma suspect and 89 glaucomatous eyes) were scanned with SD-OCT. Each SD-OCT cube dataset was first converted into 2D feature map based on retinal nerve fiber layer (RNFL) segmentation and then divided into various number of super pixels. Unlike the conventional super pixel having a fixed number of points, this newly developed variable-size super pixel is defined as a cluster of homogeneous adjacent pixels with variable size, shape and number. Features of super pixel map were extracted and used as inputs to machine classifier (LogitBoost adaptive boosting) to automatically identify diseased eyes. For discriminating performance assessment, area under the curve (AUC) of the receiver operating characteristics of the machine classifier outputs were compared with the conventional circumpapillary RNFL (cpRNFL) thickness measurements. Results: The super pixel analysis showed statistically significantly higher AUC than the cpRNFL (0.855 vs. 0.707, respectively, p = 0.031, Jackknife test) when glaucoma suspects were discriminated from healthy, while no significant difference was found when confirmed glaucoma eyes were discriminated from healthy eyes. Conclusions: A novel 3D OCT analysis technique performed at least as well as the cpRNFL in glaucoma discrimination and even better at glaucoma suspect discrimination. This new method has the potential to improve early detection of glaucomatous damage. Ā© 2013 Xu et al
Fast and Accurate Algorithm for Eye Localization for Gaze Tracking in Low Resolution Images
Iris centre localization in low-resolution visible images is a challenging
problem in computer vision community due to noise, shadows, occlusions, pose
variations, eye blinks, etc. This paper proposes an efficient method for
determining iris centre in low-resolution images in the visible spectrum. Even
low-cost consumer-grade webcams can be used for gaze tracking without any
additional hardware. A two-stage algorithm is proposed for iris centre
localization. The proposed method uses geometrical characteristics of the eye.
In the first stage, a fast convolution based approach is used for obtaining the
coarse location of iris centre (IC). The IC location is further refined in the
second stage using boundary tracing and ellipse fitting. The algorithm has been
evaluated in public databases like BioID, Gi4E and is found to outperform the
state of the art methods.Comment: 12 pages, 10 figures, IET Computer Vision, 201
Magnetic pattern at supergranulation scale: the Void Size Distribution
The large-scale magnetic pattern of the quiet sun is dominated by the
magnetic network. This network, created by photospheric magnetic fields swept
into convective downflows, delineates the boundaries of large scale cells of
overturning plasma and exhibits voids in magnetic organization. Such voids
include internetwork fields, a mixed-polarity sparse field that populate the
inner part of network cells. To single out voids and to quantify their
intrinsic pattern a fast circle packing based algorithm is applied to 511
SOHO/MDI high resolution magnetograms acquired during the outstanding solar
activity minimum between 23 and 24 cycles. The computed Void Distribution
Function shows a quasi-exponential decay behavior in the range 10-60 Mm. The
lack of distinct flow scales in such a range corroborates the hypothesis of
multi-scale motion flows at the solar surface. In addition to the
quasi-exponential decay we have found that the voids reveal departure from a
simple exponential decay around 35 Mm.Comment: 6 pages, 8 figures, to appear in Astronomy and Astrophysic
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