A Microfluidic Programmable Array for Label-free Detection of Biomolecules

One of the most promising ways to improve clinical diagnostic tools is to use microfluidic Lab-on-a-chip devices. Such devices can provide a dense array of fluidic components and sensors at the micro-scale which drastically reduce the necessary sample volumes and testing time. This dissertation develops a unique electrochemical sensor array in a microfluidic device for high-throughput, label-free detection of both DNA hybridization and protein adsorption experiments. The device consists of a patterned 3 x 3 grid of electrodes which can be individually addressed and microfluidic channels molded using the elastomer PDMS. The channels are bonded over the patterned electrodes on a silicon or glass substrate. The electrodes are designed to provide a row-column addressing format to reduce the number of contact pads required and to drastically reduce the complexity involved in scaling the device to include larger arrays. The device includes straight channels of 100 micron height which can be manually rotated to provide either horizontal or vertical fluid flow over the patterned sensors. To enhance the design of the arrayed device, a series of microvalves were integrated with the platform. This integrated system requires rounded microfluidic channels of 32 micron height and a second layer of channels which act as pneumatic valves to pinch off selected areas of the microfluidic channel. With the valves, the fluid flow direction can be controlled autonomously without moving the bonded PDMS layer. Changes to the mechanism of detection and diffusion properties of the system were examined after the integration of the microvalve network. Protein adhesion studies of three different proteins to three functionalized surfaces were performed. The electrochemical characterization data could be used to help identify adhesion properties for surface coatings used in biomedical devices or for passivating sensor surfaces. DNA hybridization experiments were performed and confirmed both arrayed and sensitive detection. Hybridization experiments performed in the valved device demonstrated an altered diffusion regime which directly affected the detection mechanism. On average, successful hybridization yielded a signal increase 8x higher than two separate control experiments. The detection limit of the sensor was calculated to be 8 nM

The Layer 0 Inner Silicon Detector of the D0 Experiment

This paper describes the design, fabrication, installation and performance of the new inner layer called Layer 0 (L0) that was inserted in the existing Run IIa Silicon Micro-Strip Tracker (SMT) of the D0 experiment at the Fermilab Tevatron collider. L0 provides tracking information from two layers of sensors, which are mounted with center lines at a radial distance of 16.1 mm and 17.6 mm respectively from the beam axis. The sensors and readout electronics are mounted on a specially designed and fabricated carbon fiber structure that includes cooling for sensor and readout electronics. The structure has a thin polyimide circuit bonded to it so that the circuit couples electrically to the carbon fiber allowing the support structure to be used both for detector grounding and a low impedance connection between the remotely mounted hybrids and the sensors.Comment: 28 pages, 9 figure

Embedded Sensors to Monitor Production of Composites : From Infusion to Curing of Resin

The need for using light-weight and high-strength fibre reinforced polymer in different applications has increased in the past few decades. The ideal product offers excellent mechanical and chemical properties with much lower weight compared to traditionally used metals. Initially, the fibre-reinforced polymers are being produced by trial and error iterations. This causes a very expensive product, with random quality and lack of reproducibility. There is a need to replace trial and error experiments with knowledge-based approaches. Using sensors for in-situ production to monitor the results in a reliable and repeatable way gives a high-quality composite product and optimizes the time and cost of the process. One of the common manufacturing processes of fibre-reinforced polymer composite is resin infusion in dry fabrics. The resin impregnates the fibrous textile through the existence of a pressure gradient in the fibrous mat, which is generated by a vacuum pump or by a resin injection at high pressure. The impregnation of the dry textile is a result of the pressure gradient between resin inlet and venting point in the mold. Therefore, the most relevant measurement to detect the resin front and the changes of resin hydrostatic pressure is measuring the pressure directly inside the laminate. In this study, pressure sensors provide real-time information about the resin front in laminate and the changes of resin hydrostatic pressure during the infusion. Different pressure sensors and interconnection techniques were examined to minimize the size of the sensing element in the composite. After complete impregnation of the fibres, the curing degree of the resin has to be measured. Microscale interdigital capacitive sensors with a perforated substrate of polyimide are designed and fabricated. The sensors are fabricated on polyimide substrate with a thickness of about 5 micrometers. The polyimide is thermally stable up to 450 degree celsius. Therefore, the sensor can be used for a variety of processes even with high-temperature curing requirements. They have a volume of around 0.1 mm3. The miniaturized dimensions of the sensor enables it to remain in the composite product with the negligible diminishing of mechanical properties. The metallization of the sensor is insulated with metal oxide built up from the metallization itself. This insulation layer enables measurement in electrically conductive carbon fibres. The sensors will remain inside the composite material for structural health monitoring during the life-time of composite. Ideally, the sensors for online process monitoring of composites should be made of the identical fibres or resin in that composite. This will eliminate the wound effect in the host material. To obtain sensorial material, a high-performance resin for aerospace application, type RTM6, is mixed with different plasticizers. The cured mixture of the resin is thin and flexible. An interdigital comb structure is screen-printed on the newly developed substrate. The curing degree of the RTM6 resin in glass and carbon fibres is measured by screen-printed planar interdigital sensor on flexible RTM6. Having sensors for online process monitoring is important for industry 4.0 to autonomously produce fibre reinforced composites in a so-called smart factory . Both, pressure sensors and interdigital capacitive sensors in this thesis can be used for online process monitoring. They will provide a knowledge-based approach for high-quality and low-cost products

Complete fabrication station of scalable microfluidic devices for sensing applications

Microfluidics has become a field of intense research in the last decades due to the interesting capabilities this type of devices have. In the sensing area, they are meant to outperform classical laboratory techniques in terms of speed, volume of sample required, resolution, handling and efficiency. However, the technology has not achieved the predicted impact on the actual sensing world. Among the issues that slow down its development, the limited scalability of the fabrication techniques used results in a poor translation from research to the market

Photonic Biosensors: Detection, Analysis and Medical Diagnostics

The role of nanotechnologies in personalized medicine is rising remarkably in the last decade because of the ability of these new sensing systems to diagnose diseases from early stages and the availability of continuous screenings to characterize the efficiency of drugs and therapies for each single patient. Recent technological advancements are allowing the development of biosensors in low-cost and user-friendly platforms, thereby overcoming the last obstacle for these systems, represented by limiting costs and low yield, until now. In this context, photonic biosensors represent one of the main emerging sensing modalities because of their ability to combine high sensitivity and selectivity together with real-time operation, integrability, and compatibility with microfluidics and electric circuitry for the readout, which is fundamental for the realization of lab-on-chip systems. This book, “Photonic Biosensors: Detection, Analysis and Medical Diagnostics”, has been published thanks to the contributions of the authors and collects research articles, the content of which is expected to assume an important role in the outbreak of biosensors in the biomedical field, considering the variety of the topics that it covers, from the improvement of sensors’ performance to new, emerging applications and strategies for on-chip integrability, aiming at providing a general overview for readers on the current advancements in the biosensing field

Sensor System for Autonomous Detection of Mold Spore Contamination

An autonomous sensor system for monitoring mold spore contaminations inside archives at desired time intervals is presented. Presence of airborne mold spores in the indoor environment not only damages organic material but it is also a potential threat to human health. To avoid this risk a sensor system for monitoring mold spore concentrations is of great importance. In this thesis, a mold sensor system with a replaceable bioreactor array for autonomous detection of the airborne spores has been investigated. The sensor system consists of a bioreactor cartridge to analyze the mold growth, an air sampling unit for distributing the mold spores into the bioreactors and a control unit to automatize the detection process. Each bioreactor is sealed with a sacrificial silicon nitride membrane, which can be opened on demand. Once activated, the membrane gets opened and the spores present in the air sample get in contact with the culture medium and start to germinate. The mold detection is performed by using an integrated approach of impedance and colorimetric principles