A handheld high-sensitivity micro-NMR CMOS platform with B-field stabilization for multi-type biological/chemical assays

We report a micro-nuclear magnetic resonance (NMR) system compatible with multi-type biological/chemical lab-on-a-chip assays. Unified in a handheld scale (dimension: 14 x 6 x 11 cm³, weight: 1.4 kg), the system is capable to detect<100 pM of Enterococcus faecalis derived DNA from a 2.5 μL sample. The key components are a portable magnet (0.46 T, 1.25 kg) for nucleus magnetization, a system PCB for I/O interface, an FPGA for system control, a current driver for trimming the magnetic (B) field, and a silicon chip fabricated in 0.18 μm CMOS. The latter, integrated with a current-mode vertical Hall sensor and a low-noise readout circuit, facilitates closed-loop B-field stabilization (2 mT → 0.15 mT), which otherwise fluctuates with temperature or sample displacement. Together with a dynamic-B-field transceiver with a planar coil for micro-NMR assay and thermal control, the system demonstrates: 1) selective biological target pinpointing; 2) protein state analysis; and 3) solvent-polymer dynamics, suitable for healthcare, food and colloidal applications, respectively. Compared to a commercial NMR-assay product (Bruker mq-20), this platform greatly reduces the sample consumption (120x), hardware volume (175x), and weight (96x)

Laboratory Protocols CIMMYT Applied Molecular Genetics Laboratory

chemiluminescence immunoassays, DNA, DNA hybridization, molecular markers, PCR, RFLPs, Research and Development/Tech Change/Emerging Technologies, F30,

Impact of remote sensing upon the planning, management, and development of water resources

Principal water resources users were surveyed to determine the impact of remote data streams on hydrologic computer models. Analysis of responses demonstrated that: most water resources effort suitable to remote sensing inputs is conducted through federal agencies or through federally stimulated research; and, most hydrologic models suitable to remote sensing data are federally developed. Computer usage by major water resources users was analyzed to determine the trends of usage and costs for the principal hydrologic users/models. The laws and empirical relationships governing the growth of the data processing loads were described and applied to project the future data loads. Data loads for ERTS CCT image processing were computed and projected through the 1985 era

The indirect effects of manufacturing internationalization on logistics - Evidence from the Italian districts

The aim of the paper is to investigate the indirect impact of internationalisation process, undertaken by the district firms located in Veneto region (north-eastern Italy), on the logistics’ employment change within the same industrial districts. The results of the empirical analysis have showed that within the districts there is not a clear positive relationship between internationalisation and the employment growth in the logistics sector. This might be due, either to a strong insourcing of logistics activities by district firms, or to a trend of outsourcing such activities to logistics suppliers located outside the industrial districts. The paper is organised into five sections. An introduction is followed by a literary review on the direct and indirect effects of manufacturing internationalisation on the home country, with a specific focus on logistics. The sample and the methodology are described in section three, section four presents the empirical results. Conclusions and further research questions follow.internationalisation, industrial districts, logistics sector, indirect effects.

Standard methods for molecular research in Apis mellifera

From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.Fundação para a Ciência e Tecnologi

A new GIS toolbox for integrating massive heterogeneous GIS data for land use change analysis

Agricultural land use in Germany and related impacts on the environment and the use of natural resources are key research topics at the Thünen-Institute of Rural Studies. As spatial context is essential for the analysis of causal connections, GIS data regarding all necessary information was gathered during different research projects and prepared for processing in a database. In particular, the Integrated Administration and Control System, which was available for certain project purposes for several Federal Laender and years, serves as a very detailed data source for agricultural land use. We use different Open Source GIS software like PostgreSQL/ PostGIS, GRASS and QuantumGIS for geoprocessing, supplemented with the proprietary ESRI product ArcGIS. After introducing the used input data and the general processing approach, this paper presents a selection of geoprocessing routines for which Open Source GIS software was used. As an exemplary ’use case’ for the conclusions from the consecutive statistical analysis, we summarize impacts of increased biogas production on agricultural land use change highlighting the trend in biogas maize cultivation and the conversion of permanent grassland to agricultural cropland

High performance millimeter-wave microstrip circulators and isolators

Millimeter wave systems, phased array antennas, and high performance components all require wideband circulators (and isolators) to perform diplexing and switching, to improve isolation and Voltage Standing Wave Ratio (VSWR), and to construct IMPATT diode reflection amplifiers. Presently, most of the millimeter-wave circulators and isolators are available in the configurations of waveguide or stripline, both of which suffer from the shortcomings of bulky size/weight, narrow bandwidth, and poor compatibility with monolithic millimeter-wave integrated circuits (MMIC). MMW microstrip circulators/isolators can eliminate or improve these shortcomings. Stub-tuned microstrip circulator configuration were developed utilizing the electromagnetic fields perturbation technique, the adhesion problems of microstrip metallization on new ferrite substrate were overcome, the fabrication, assembly, packaging techniques were improved, and then successfully designed, fabricated a Ka band circulator which has isolation and return loss of greater than 16dB, insertion loss less than 0.7dB. To assess the steady and reliable performance of the circulator, a temperature cycling test was done over the range of -20 to +50 C for 3 continuous cycles and found no significant impact or variation of circulator performance

Impact of remote sensing upon the planning, management, and development of water resources

A survey of the principal water resource users was conducted to determine the impact of new remote data streams on hydrologic computer models. The analysis of the responses and direct contact demonstrated that: (1) the majority of water resource effort of the type suitable to remote sensing inputs is conducted by major federal water resources agencies or through federally stimulated research, (2) the federal government develops most of the hydrologic models used in this effort; and (3) federal computer power is extensive. The computers, computer power, and hydrologic models in current use were determined

The supernova-regulated ISM. I. The multi-phase structure

We simulate the multi-phase interstellar medium randomly heated and stirred by supernovae, with gravity, differential rotation and other parameters of the solar neighbourhood. Here we describe in detail both numerical and physical aspects of the model, including injection of thermal and kinetic energy by SN explosions, radiative cooling, photoelectric heating and various transport processes. With 3D domain extending 1 kpc^2 horizontally and 2 kpc vertically, the model routinely spans gas number densities 10^-5 - 10^2 cm^-3, temperatures 10-10^8 K, local velocities up to 10^3 km s^-1 (with Mach number up to 25). The thermal structure of the modelled ISM is classified by inspection of the joint probability density of the gas number density and temperature. We confirm that most of the complexity can be captured in terms of just three phases, separated by temperature borderlines at about 10^3 K and 5x10^5 K. The probability distribution of gas density within each phase is approximately lognormal. We clarify the connection between the fractional volume of a phase and its various proxies, and derive an exact relation between the fractional volume and the filling factors defined in terms of the volume and probabilistic averages. These results are discussed in both observational and computational contexts. The correlation scale of the random flows is calculated from the velocity autocorrelation function; it is of order 100 pc and tends to grow with distance from the mid-plane. We use two distinct parameterizations of radiative cooling to show that the multi-phase structure of the gas is robust, as it does not depend significantly on this choice.Comment: 28 pages, 22 figures and 8 table