7,187 research outputs found
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Archiving and disseminating integrative structure models.
Limitations in the applicability, accuracy, and precision of individual structure characterization methods can sometimes be overcome via an integrative modeling approach that relies on information from all available sources, including all available experimental data and prior models. The open-source Integrative Modeling Platform (IMP) is one piece of software that implements all computational aspects of integrative modeling. To maximize the impact of integrative structures, the coordinates should be made publicly available, as is already the case for structures based on X-ray crystallography, NMR spectroscopy, and electron microscopy. Moreover, the associated experimental data and modeling protocols should also be archived, such that the original results can easily be reproduced. Finally, it is essential that the integrative structures are validated as part of their publication and deposition. A number of research groups have already developed software to implement integrative modeling and have generated a number of structures, prompting the formation of an Integrative/Hybrid Methods Task Force. Following the recommendations of this task force, the existing PDBx/mmCIF data representation used for atomic PDB structures has been extended to address the requirements for archiving integrative structural models. This IHM-dictionary adds a flexible model representation, including coarse graining, models in multiple states and/or related by time or other order, and multiple input experimental information sources. A prototype archiving system called PDB-Dev ( https://pdb-dev.wwpdb.org ) has also been created to archive integrative structural models, together with a Python library to facilitate handling of integrative models in PDBx/mmCIF format
Conformational states of macromolecular assemblies explored by integrative structure calculation
A detailed description of macromolecular assemblies in multiple conformational states can be very valuable for understanding cellular processes. At present, structural determination of most assemblies in different biologically relevant conformations cannot be achieved by a single technique and thus requires an integrative approach that combines information from multiple sources. Different techniques require different computational methods to allow efficient and accurate data processing and analysis. Here, we summarize the latest advances and future challenges in computational methods that help the interpretation of data from two techniquesâmass spectrometry and three-dimensional cryo-electron microscopy (with focus on alignment and classification of heterogeneous subtomograms from cryo-electron tomography). We evaluate how new developments in these two broad fields will lead to further integration with atomic structures to broaden our picture of the dynamic behavior of assemblies in their native environment
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Structural basis of mitochondrial receptor binding and constriction by DRP1.
Mitochondrial inheritance, genome maintenance and metabolic adaptation depend on organelle fission by dynamin-related protein 1 (DRP1) and its mitochondrial receptors. DRP1 receptors include the paralogues mitochondrial dynamics proteins of 49 and 51 kDa (MID49 and MID51) and mitochondrial fission factor (MFF); however, the mechanisms by which these proteins recruit and regulate DRP1 are unknown. Here we present a cryo-electron microscopy structure of full-length human DRP1 co-assembled with MID49 and an analysis of structure- and disease-based mutations. We report that GTP induces a marked elongation and rotation of the GTPase domain, bundle-signalling element and connecting hinge loops of DRP1. In this conformation, a network of multivalent interactions promotes the polymerization of a linear DRP1 filament with MID49 or MID51. After co-assembly, GTP hydrolysis and exchange lead to MID receptor dissociation, filament shortening and curling of DRP1 oligomers into constricted and closed rings. Together, these views of full-length, receptor- and nucleotide-bound conformations reveal how DRP1 performs mechanical work through nucleotide-driven allostery
Unsupervised cryo-EM data clustering through adaptively constrained K-means algorithm
In single-particle cryo-electron microscopy (cryo-EM), K-means clustering
algorithm is widely used in unsupervised 2D classification of projection images
of biological macromolecules. 3D ab initio reconstruction requires accurate
unsupervised classification in order to separate molecular projections of
distinct orientations. Due to background noise in single-particle images and
uncertainty of molecular orientations, traditional K-means clustering algorithm
may classify images into wrong classes and produce classes with a large
variation in membership. Overcoming these limitations requires further
development on clustering algorithms for cryo-EM data analysis. We propose a
novel unsupervised data clustering method building upon the traditional K-means
algorithm. By introducing an adaptive constraint term in the objective
function, our algorithm not only avoids a large variation in class sizes but
also produces more accurate data clustering. Applications of this approach to
both simulated and experimental cryo-EM data demonstrate that our algorithm is
a significantly improved alterative to the traditional K-means algorithm in
single-particle cryo-EM analysis.Comment: 35 pages, 14 figure
Structure-function mapping of a heptameric module in the nuclear pore complex.
The nuclear pore complex (NPC) is a multiprotein assembly that serves as the sole mediator of nucleocytoplasmic exchange in eukaryotic cells. In this paper, we use an integrative approach to determine the structure of an essential component of the yeast NPC, the ~600-kD heptameric Nup84 complex, to a precision of ~1.5 nm. The configuration of the subunit structures was determined by satisfaction of spatial restraints derived from a diverse set of negative-stain electron microscopy and protein domain-mapping data. Phenotypic data were mapped onto the complex, allowing us to identify regions that stabilize the NPC's interaction with the nuclear envelope membrane and connect the complex to the rest of the NPC. Our data allow us to suggest how the Nup84 complex is assembled into the NPC and propose a scenario for the evolution of the Nup84 complex through a series of gene duplication and loss events. This work demonstrates that integrative approaches based on low-resolution data of sufficient quality can generate functionally informative structures at intermediate resolution
Mahalanobis Distance for Class Averaging of Cryo-EM Images
Single particle reconstruction (SPR) from cryo-electron microscopy (EM) is a
technique in which the 3D structure of a molecule needs to be determined from
its contrast transfer function (CTF) affected, noisy 2D projection images taken
at unknown viewing directions. One of the main challenges in cryo-EM is the
typically low signal to noise ratio (SNR) of the acquired images. 2D
classification of images, followed by class averaging, improves the SNR of the
resulting averages, and is used for selecting particles from micrographs and
for inspecting the particle images. We introduce a new affinity measure, akin
to the Mahalanobis distance, to compare cryo-EM images belonging to different
defocus groups. The new similarity measure is employed to detect similar
images, thereby leading to an improved algorithm for class averaging. We
evaluate the performance of the proposed class averaging procedure on synthetic
datasets, obtaining state of the art classification.Comment: Final version accepted to the 14th IEEE International Symposium on
Biomedical Imaging (ISBI 2017
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