Automatic Classification of Human Epithelial Type 2 Cell Indirect Immunofluorescence Images using Cell Pyramid Matching

This paper describes a novel system for automatic classification of images obtained from Anti-Nuclear Antibody (ANA) pathology tests on Human Epithelial type 2 (HEp-2) cells using the Indirect Immunofluorescence (IIF) protocol. The IIF protocol on HEp-2 cells has been the hallmark method to identify the presence of ANAs, due to its high sensitivity and the large range of antigens that can be detected. However, it suffers from numerous shortcomings, such as being subjective as well as time and labour intensive. Computer Aided Diagnostic (CAD) systems have been developed to address these problems, which automatically classify a HEp-2 cell image into one of its known patterns (eg. speckled, homogeneous). Most of the existing CAD systems use handpicked features to represent a HEp-2 cell image, which may only work in limited scenarios. We propose a novel automatic cell image classification method termed Cell Pyramid Matching (CPM), which is comprised of regional histograms of visual words coupled with the Multiple Kernel Learning framework. We present a study of several variations of generating histograms and show the efficacy of the system on two publicly available datasets: the ICPR HEp-2 cell classification contest dataset and the SNPHEp-2 dataset.Comment: arXiv admin note: substantial text overlap with arXiv:1304.126

Classification of Human Epithelial Type 2 Cell Indirect Immunofluoresence Images via Codebook Based Descriptors

The Anti-Nuclear Antibody (ANA) clinical pathology test is commonly used to identify the existence of various diseases. A hallmark method for identifying the presence of ANAs is the Indirect Immunofluorescence method on Human Epithelial (HEp-2) cells, due to its high sensitivity and the large range of antigens that can be detected. However, the method suffers from numerous shortcomings, such as being subjective as well as time and labour intensive. Computer Aided Diagnostic (CAD) systems have been developed to address these problems, which automatically classify a HEp-2 cell image into one of its known patterns (eg., speckled, homogeneous). Most of the existing CAD systems use handpicked features to represent a HEp-2 cell image, which may only work in limited scenarios. In this paper, we propose a cell classification system comprised of a dual-region codebook-based descriptor, combined with the Nearest Convex Hull Classifier. We evaluate the performance of several variants of the descriptor on two publicly available datasets: ICPR HEp-2 cell classification contest dataset and the new SNPHEp-2 dataset. To our knowledge, this is the first time codebook-based descriptors are applied and studied in this domain. Experiments show that the proposed system has consistent high performance and is more robust than two recent CAD systems

HEP-2 CELL IMAGES FLUORESCENCE INTENSITY CLASSIFICATION TO DETERMINE POSITIVITY BASED ON NEURAL NETWORK AMIN

Nowadays, the recommended method for detection of anti-nuclear auto-antibodies is by using Indirect Immunofluorescence (IIF). The increasing of test demands on classification of Hep-2 cell images force the physicians to carry out the test faster, resulting bad quality results. IIF diagnosis requires estimating the fluorescence intensity of the serum and this will be observed. As there are subjective and inter/intra laboratory perception of the results, the development of computer-aided diagnosis (CAD) tools is used to support the decision. In this report, we propose the classification technique based on Artificial Neural Network (ANN) that can classify the Hep-2 cell images into 3 classes namely positive, negative and intermediate,specifically to determine the presence of antinuclear autoantibodies (ANA)

Pattern Classification of Human Epithelial Images

This project shows an important role to diagnosis autoimmune disorder which is by a comparative analysis on the most appropriate clustering technique for the segmentation and also to develop algorithm for positivity classification. In this project, there are four stages will be used to analyze pattern classification in human epithelial (HEp-2) images. First of all, image enhancement will take part in order to boost efficiency of algorithm by implementing some of the adjustment and filtering technique to increase the visibility of image. After that, the second stage will be the image segmentation by using most appropriate clustering technique. There will be a comparative analysis on clustering techniques for segmentation which are adaptive fuzzy c-mean and adaptive fuzzy moving k-mean. Then, for features extraction, by calculating the mean of each of the properties such as area, perimeter, major axis length, and minor axis length for each images. After that, will implementing a grouping based on properties dataset that has been calculated. Last but not least, from the mean of properties, it will classify into the pattern after ranging the value of mean properties of each of the pattern itself that has been done in classification stage

Pattern Classification of Human Epithelial Images

This project shows an important role to diagnosis autoimmune disorder which is by a comparative analysis on the most appropriate clustering technique for the segmentation and also to develop algorithm for positivity classification. In this project, there are four stages will be used to analyze pattern classification in human epithelial (HEp-2) images. First of all, image enhancement will take part in order to boost efficiency of algorithm by implementing some of the adjustment and filtering technique to increase the visibility of image. After that, the second stage will be the image segmentation by using most appropriate clustering technique. There will be a comparative analysis on clustering techniques for segmentation which are adaptive fuzzy c-mean and adaptive fuzzy moving k-mean. Then, for features extraction, by calculating the mean of each of the properties such as area, perimeter, major axis length, and minor axis length for each images. After that, will implementing a grouping based on properties dataset that has been calculated. Last but not least, from the mean of properties, it will classify into the pattern after ranging the value of mean properties of each of the pattern itself that has been done in classification stage

HEP-2 CELL IMAGES FLUORESCENCE INTENSITY CLASSIFICATION TO DETERMINE POSITIVITY BASED ON NEURAL NETWORK AMIN

Nowadays, the recommended method for detection of anti-nuclear auto-antibodies is by using Indirect Immunofluorescence (IIF). The increasing of test demands on classification of Hep-2 cell images force the physicians to carry out the test faster, resulting bad quality results. IIF diagnosis requires estimating the fluorescence intensity of the serum and this will be observed. As there are subjective and inter/intra laboratory perception of the results, the development of computer-aided diagnosis (CAD) tools is used to support the decision. In this report, we propose the classification technique based on Artificial Neural Network (ANN) that can classify the Hep-2 cell images into 3 classes namely positive, negative and intermediate,specifically to determine the presence of antinuclear autoantibodies (ANA)

An Intelligent Detection System for Rheumatoid Arthritis (RA) Disease using Image Processing

Rheumatoid Arthritis (RA) is an autoimmune disease that causes chronic pain, stiffness, redness or loss of function in the joints. Other than early diagnosis, there is yet a cure available for RA. Diseases with similar symptoms such as lupus, osteoarthritis, gout cause difficulty in diagnosing RA. Currently, indirect immunofluorescence (IIF) test performed to identify ANA in Hep-2 cells. Thus, image processing techniques vital to make diagnosis more efficient, accurate and less time-consuming. For this project standardized staining pattern classifier to be designed by using image processing techniques. Current manual techniques has limited accuracy and time consuming. In IFF procedures, unsuitable microscope to read Hep-2 cell slides, or photo bleaching effect where cells bleached extremely in short period of time are disadvantages. Another downside is test results being subject to change with experts knowledge and years of experience. These factors lead to low accuracy and it becomes a lengthy process due to large number of images. Out of five types of staining patterns nucleolar and centromere share similar visual appearance and the same is true to homogeneous, fine-speckled, coarse-speckled patterns. This is one of the major factors affecting classification accuracy due to results being subjective. In this research, First and Second Order Statistics Feature Extraction, Mamdani Fuzzy Logic Classification methods utilized to develop automatic detection system for RA with the help of Matlab R2012b, Fuzzy Logic Toolbox, and Image Processing Toolbox. The algorithm tested on the publicly available Mivia Hep-2 Cell image dataset. Fuzzy logic classified 85 out of 250 images wrongly. It has 66% accuracy. The images obtained from MIVIA dataset has been manually segmented to cell level from the image level. Developing an automated segmentation algorithm might give better results

An Intelligent Detection System for Rheumatoid Arthritis (RA) Disease using Image Processing

Rheumatoid Arthritis (RA) is an autoimmune disease that causes chronic pain, stiffness, redness or loss of function in the joints. Other than early diagnosis, there is yet a cure available for RA. Diseases with similar symptoms such as lupus, osteoarthritis, gout cause difficulty in diagnosing RA. Currently, indirect immunofluorescence (IIF) test performed to identify ANA in Hep-2 cells. Thus, image processing techniques vital to make diagnosis more efficient, accurate and less time-consuming. For this project standardized staining pattern classifier to be designed by using image processing techniques. Current manual techniques has limited accuracy and time consuming. In IFF procedures, unsuitable microscope to read Hep-2 cell slides, or photo bleaching effect where cells bleached extremely in short period of time are disadvantages. Another downside is test results being subject to change with experts knowledge and years of experience. These factors lead to low accuracy and it becomes a lengthy process due to large number of images. Out of five types of staining patterns nucleolar and centromere share similar visual appearance and the same is true to homogeneous, fine-speckled, coarse-speckled patterns. This is one of the major factors affecting classification accuracy due to results being subjective. In this research, First and Second Order Statistics Feature Extraction, Mamdani Fuzzy Logic Classification methods utilized to develop automatic detection system for RA with the help of Matlab R2012b, Fuzzy Logic Toolbox, and Image Processing Toolbox. The algorithm tested on the publicly available Mivia Hep-2 Cell image dataset. Fuzzy logic classified 85 out of 250 images wrongly. It has 66% accuracy. The images obtained from MIVIA dataset has been manually segmented to cell level from the image level. Developing an automated segmentation algorithm might give better results