Two-photon imaging and analysis of neural network dynamics

The glow of a starry night sky, the smell of a freshly brewed cup of coffee or the sound of ocean waves breaking on the beach are representations of the physical world that have been created by the dynamic interactions of thousands of neurons in our brains. How the brain mediates perceptions, creates thoughts, stores memories and initiates actions remains one of the most profound puzzles in biology, if not all of science. A key to a mechanistic understanding of how the nervous system works is the ability to analyze the dynamics of neuronal networks in the living organism in the context of sensory stimulation and behaviour. Dynamic brain properties have been fairly well characterized on the microscopic level of individual neurons and on the macroscopic level of whole brain areas largely with the help of various electrophysiological techniques. However, our understanding of the mesoscopic level comprising local populations of hundreds to thousands of neurons (so called 'microcircuits') remains comparably poor. In large parts, this has been due to the technical difficulties involved in recording from large networks of neurons with single-cell spatial resolution and near- millisecond temporal resolution in the brain of living animals. In recent years, two-photon microscopy has emerged as a technique which meets many of these requirements and thus has become the method of choice for the interrogation of local neural circuits. Here, we review the state-of-research in the field of two-photon imaging of neuronal populations, covering the topics of microscope technology, suitable fluorescent indicator dyes, staining techniques, and in particular analysis techniques for extracting relevant information from the fluorescence data. We expect that functional analysis of neural networks using two-photon imaging will help to decipher fundamental operational principles of neural microcircuits.Comment: 36 pages, 4 figures, accepted for publication in Reports on Progress in Physic

AFFECT-PRESERVING VISUAL PRIVACY PROTECTION

The prevalence of wireless networks and the convenience of mobile cameras enable many new video applications other than security and entertainment. From behavioral diagnosis to wellness monitoring, cameras are increasing used for observations in various educational and medical settings. Videos collected for such applications are considered protected health information under privacy laws in many countries. Visual privacy protection techniques, such as blurring or object removal, can be used to mitigate privacy concern, but they also obliterate important visual cues of affect and social behaviors that are crucial for the target applications. In this dissertation, we propose to balance the privacy protection and the utility of the data by preserving the privacy-insensitive information, such as pose and expression, which is useful in many applications involving visual understanding. The Intellectual Merits of the dissertation include a novel framework for visual privacy protection by manipulating facial image and body shape of individuals, which: (1) is able to conceal the identity of individuals; (2) provide a way to preserve the utility of the data, such as expression and pose information; (3) balance the utility of the data and capacity of the privacy protection. The Broader Impacts of the dissertation focus on the significance of privacy protection on visual data, and the inadequacy of current privacy enhancing technologies in preserving affect and behavioral attributes of the visual content, which are highly useful for behavior observation in educational and medical settings. This work in this dissertation represents one of the first attempts in achieving both goals simultaneously

Cross-calibration of Time-of-flight and Colour Cameras

Time-of-flight cameras provide depth information, which is complementary to the photometric appearance of the scene in ordinary images. It is desirable to merge the depth and colour information, in order to obtain a coherent scene representation. However, the individual cameras will have different viewpoints, resolutions and fields of view, which means that they must be mutually calibrated. This paper presents a geometric framework for this multi-view and multi-modal calibration problem. It is shown that three-dimensional projective transformations can be used to align depth and parallax-based representations of the scene, with or without Euclidean reconstruction. A new evaluation procedure is also developed; this allows the reprojection error to be decomposed into calibration and sensor-dependent components. The complete approach is demonstrated on a network of three time-of-flight and six colour cameras. The applications of such a system, to a range of automatic scene-interpretation problems, are discussed.Comment: 18 pages, 12 figures, 3 table

Proceedings of the 2019 Joint Workshop of Fraunhofer IOSB and Institute for Anthropomatics, Vision and Fusion Laboratory

In 2019 fand wieder der jährliche Workshop des Fraunhofer IOSB und des Lehrstuhls für Interaktive Echtzeitsysteme des Karlsruher Insitut für Technologie statt. Die Doktoranden beider Institutionen präsentierten den Fortschritt ihrer Forschung in den Themen Maschinelles Lernen, Machine Vision, Messtechnik, Netzwerksicherheit und Usage Control. Die Ideen dieses Workshops sind in diesem Buch gesammelt in der Form technischer Berichte

Affine multi-view modelling for close range object measurement

In photogrammetry, sensor modelling with 3D point estimation is a fundamental topic of research. Perspective frame cameras offer the mathematical basis for close range modelling approaches. The norm is to employ robust bundle adjustments for simultaneous parameter estimation and 3D object measurement. In 2D to 3D modelling strategies image resolution, scale, sampling and geometric distortion are prior factors. Non-conventional image geometries that implement uncalibrated cameras are established in computer vision approaches; these aim for fast solutions at the expense of precision. The projective camera is defined in homogeneous terms and linear algorithms are employed. An attractive sensor model disembodied from projective distortions is the affine. Affine modelling has been studied in the contexts of geometry recovery, feature detection and texturing in vision, however multi-view approaches for precise object measurement are not yet widely available. This project investigates affine multi-view modelling from a photogrammetric standpoint. A new affine bundle adjustment system has been developed for point-based data observed in close range image networks. The system allows calibration, orientation and 3D point estimation. It is processed as a least squares solution with high redundancy providing statistical analysis. Starting values are recovered from a combination of implicit perspective and explicit affine approaches. System development focuses on retrieval of orientation parameters, 3D point coordinates and internal calibration with definition of system datum, sensor scale and radial lens distortion. Algorithm development is supported with method description by simulation. Initialization and implementation are evaluated with the statistical indicators, algorithm convergence and correlation of parameters. Object space is assessed with evaluation of the 3D point correlation coefficients and error ellipsoids. Sensor scale is checked with comparison of camera systems utilizing quality and accuracy metrics. For independent method evaluation, testing is implemented over a perspective bundle adjustment tool with similar indicators. Test datasets are initialized from precise reference image networks. Real affine image networks are acquired with an optical system (~1M pixel CCD cameras with 0.16x telecentric lens). Analysis of tests ascertains that the affine method results in an RMS image misclosure at a sub-pixel level and precisions of a few tenths of microns in object space

Proceedings of the 2019 Joint Workshop of Fraunhofer IOSB and Institute for Anthropomatics, Vision and Fusion Laboratory

In 2019 again, the annual joint workshop of the Fraunhofer IOSB and the Vision and Fusion Laboratory of the Karlsruhe Institute of Technology took place. The doctoral students of both institutions presented extensive reports on the status of their research and discussed topics ranging from computer vision and optical metrology to network security, usage control and machine learning. The results and ideas presented at the workshop are collected in this book in the form of technical reports