Functional near infrared spectroscopy (fNIRS) to assess cognitive function in infants in rural Africa

Cortical mapping of cognitive function during infancy is poorly understood in low-income countries due to the lack of transportable neuroimaging methods. We have successfully piloted functional near infrared spectroscopy (fNIRS) as a neuroimaging tool in rural Gambia. Four-to-eight month old infants watched videos of Gambian adults perform social movements, while haemodynamic responses were recorded using fNIRS. We found distinct regions of the posterior superior temporal and inferior frontal cortex that evidenced either visual-social activation or vocally selective activation (vocal > non-vocal). The patterns of selective cortical activation in Gambian infants replicated those observed within similar aged infants in the UK. These are the first reported data on the measurement of localized functional brain activity in young infants in Africa and demonstrate the potential that fNIRS offers for field-based neuroimaging research of cognitive function in resource-poor rural communities

Functional near infrared spectroscopy (fNIRS) to assess cognitive function in infants in rural Africa

Cortical mapping of cognitive function during infancy is poorly understood in low-income countries due to the lack of transportable neuroimaging methods. We have successfully piloted functional near infrared spectroscopy (fNIRS) as a neuroimaging tool in rural Gambia. Four-to-eight month old infants watched videos of Gambian adults perform social movements, while haemodynamic responses were recorded using fNIRS. We found distinct regions of the posterior superior temporal and inferior frontal cortex that evidenced either visual-social activation or vocally selective activation (vocal > non-vocal). The patterns of selective cortical activation in Gambian infants replicated those observed within similar aged infants in the UK. These are the first reported data on the measurement of localized functional brain activity in young infants in Africa and demonstrate the potential that fNIRS offers for field-based neuroimaging research of cognitive function in resource-poor rural communities

Measuring engagement of the executive control network from 3 months of age

The executive control network (ECN) is critical for higher cognition and executive function (EF). Despite its importance, no scientific consensus has been reached on how and when it begins to function. In the present study, we assessed the development of the ECN in awake infants less than a year old by employing functional magnetic resonance imaging (fMRI) and naturalistic stimuli. First, we identified evocative movies that engaged infant attention. We then transferred them into adult imaging to test for which movie evoked the highest ECN response. Strong ECN responses were evoked while viewing Despicable Me, therefore we implemented this movie into infant imaging. We found that as early as 3-months of age, infants showed a similar response to that of adults. Overall, we demonstrated a technique that could potentially gauge EF in very young infants and developed a tool capable of imaging awake infants under natural conditions

Optical imaging during toddlerhood: brain responses during naturalistic social interactions

Despite the importance of our ability to interact and communicate with others, the early development of the social brain network remains poorly understood. We examined brain activity in 12- to 14-month-old infants while they were interacting live with an adult in two different naturalistic social scenarios (i.e., reading a picture book versus singing nursery rhymes with gestures), as compared to baseline (i.e., showing infants a toy without eye contact or speech). We used functional near-infrared spectroscopy (fNIRS) recorded over the right temporal lobe of infants to assess the role of the superior temporal sulcus - temporoparietal junction (STS-TPJ) region during naturalistic social interactions. We observed increased cortical activation in the STS-TPJ region to live social stimuli in both socially engaging conditions compared to baseline during real life interaction, with greater activation evident for the joint attention (reading book) condition relative to the social nursery rhymes. These results supported the view that the STS-TPJ region, engaged in the cortical social brain network, is already specialized in infants for processing social signals and is sensitive to communicative situations. This study also highlighted the potential of fNIRS for studying brain function in infants entering toddlerhood during live social interaction

Are you talking to me? Neural activations in 6-month-old infants in response to being addressed during natural interactions

Human interactions are guided by continuous communication among the parties involved, in which verbal communication plays a primary role. However, speech does not necessarily reveal to whom it is addressed, especially for young infants who are unable to decode its semantic content. To overcome such difficulty, adults often explicitly mark their communication as infant-directed. In the present study we investigated whether ostensive signals, which would disambiguate the infant as the addressee of a communicative act, would modulate the brain responses of 6-month-old infants to speech and gestures in an ecologically valid setting. In Experiment 1, we tested whether the gaze direction of the speaker modulates cortical responses to infant-direct speech. To provide a naturalistic environment, two infants and their parents participated at the same time. In Experiment 2, we tested whether a similar modulation of the cortical response would be obtained by varying the intonation (infant versus adult directed speech) of the speech during face-to-face communication, one on one. The results of both experiments indicated that only the combination of ostensive signals (infant directed speech and direct gaze) led to enhanced brain activation. This effect was indicated by responses localized in regions known to be involved in processing auditory and visual aspects of social communication. This study also demonstrated the potential of fNIRS as a tool for studying neural responses in naturalistic scenarios, and for simultaneous measurement of brain function in multiple participants

Neural entrainment to continuous speech and language processing in the early years of life

This thesis aimed to explore the neural mechanisms of language processing in infants under 12 months of age by using EEG measures of speech processing. More specifically, I wanted to investigate if infants are able to engage in the auditory neural tracking of continuous speech and how this processing can be modulated by infant attention and different linguistic environments. Limited research has investigated this phenomenon of neural tracking in infants and the potential effects that this may have on later language development. Experiment 1 set the groundwork for the thesis by establishing a reliable method to measure cortical entrainment by 36 infants to the amplitude envelope of continuous speech. The results demonstrated that infants have entrainment to speech much like has been found in adults. Additionally, infants show a reliable elicitation of the Acoustic Change Complex (ACC). Follow up language assessments were conducted with these infants approximately two years later; however, no significant predictors of coherence on later language outcomes were found. The aim of Experiment 2 was to discover how neural entrainment can be modulated by infant attention. Twenty infants were measured on their ability to selectively attend to a target speaker while in the presence of a distractor of matching acoustic intensity. Coherence values were found for the target, the distractor and for the dual signal (both target and distractor together). Thus, it seems that infant attention may be fluctuating between the two speech signals leading to them entraining to both simultaneously. However, the results were not clear. Thus, Experiment 3 expanded on from Experiment 2. However, now EEG was recorded from 30 infants who listened to speech with no acoustic interference and speech-in-noise with a signal-to-noise ratio of 10dB. Additionally, it was investigated whether bilingualism has any potential effects on this process. Similar coherence values were observed when infants listened to speech in both conditions (quiet and noise), suggesting that infants successfully inhibited the disruptive effects of the masker. No effects of bilingualism on neural entrainment were present. For the fourth study we wanted to continue investigating infant auditory-neural entrainment when exposed to more varying levels of background noise. However, due to the COVID-19 pandemic all testing was moved online. Thus, for Experiment 4 we developed a piece of online software (the memory card game) that could be used remotely. Seventy three children ranging from 4 to 12 years old participated in the online experiment in order to explore how the demands of a speech recognition task interact with masker type and language and how this changes with age during childhood. Results showed that performance on the memory card game improved with age but was not affected by masker type or language background. This improvement with age is most likely a result of improved speech perception capabilities. Overall, this thesis provides a reliable methodology for measuring neural entrainment in infants and a greater understanding of the mechanisms of speech processing in infancy and beyond

Natural infant-directed speech facilitates neural tracking of prosody

Infants prefer to be addressed with infant-directed speech (IDS). IDS benefits language acquisition through amplified low-frequency amplitude modulations. It has been reported that this amplification increases electrophysiological tracking of IDS compared to adult-directed speech (ADS). It is still unknown which particular frequency band triggers this effect. Here, we compare tracking at the rates of syllables and prosodic stress, which are both critical to word segmentation and recognition. In mother-infant dyads (n=30), mothers described novel objects to their 9-month-olds while infants’ EEG was recorded. For IDS, mothers were instructed to speak to their children as they typically do, while for ADS, mothers described the objects as if speaking with an adult. Phonetic analyses confirmed that pitch features were more prototypically infant-directed in the IDS-condition compared to the ADS-condition. Neural tracking of speech was assessed by speech-brain coherence, which measures the synchronization between speech envelope and EEG. Results revealed significant speech-brain coherence at both syllabic and prosodic stress rates, indicating that infants track speech in IDS and ADS at both rates. We found significantly higher speech-brain coherence for IDS compared to ADS in the prosodic stress rate but not the syllabic rate. This indicates that the IDS benefit arises primarily from enhanced prosodic stress. Thus, neural tracking is sensitive to parents’ speech adaptations during natural interactions, possibly facilitating higher-level inferential processes such as word segmentation from continuous speech

Modulation of EEG theta by naturalistic social content is not altered in infants with family history of autism

Theta oscillations (spectral power and connectivity) are sensitive to the social content of an experience in typically developing infants, providing a possible marker of early social brain development. Autism is a neurodevelopmental condition affecting early social behaviour, but links to underlying social brain function remain unclear. We explored whether modulations of theta spectral power and connectivity by naturalistic social content in infancy are related to family history for autism. Fourteen-month-old infants with (family history; FH; N = 75) and without (no family history; NFH; N = 26) a first-degree relative with autism watched social and non-social videos during EEG recording. We calculated theta (4–5 Hz) spectral power and connectivity modulations (social–non-social) and associated them with outcomes at 36 months. We replicated previous findings of increased theta power and connectivity during social compared to non-social videos. Theta modulations with social content were similar between groups, for both power and connectivity. Together, these findings suggest that neural responses to naturalistic social stimuli may not be strongly altered in 14-month-old infants with family history of autism

Educational neuroscience: Neural structure-mapping and the promise of oscillations

A critical goal for educational neuroscience is to specify causal developmental mechanisms of learning. In-depth understanding of neural information coding and transmission should enable understanding of how sensory systems build the cognitive systems critical for education–language, attention, memory-over developmental time. Cortical oscillatory encoding processes may offer mechanistic insights into how developmental trajectories unfold. All educators are familiar with children who show mastery of taught information one week, yet subsequently appear to lose it. Oscillatory research shows that sensory information that arrives out of phase with ongoing oscillations does not reach awareness, while complex combinations of bottom-up and top-down phase-phase and phase-power relations enable context and prior knowledge to influence current performance. Oscillatory studies of language are used to illustrate the promise of oscillations research for education.MRC (G0902375)This is the author accepted manuscript. The final version is available from Elsevier via http://dx.doi.org/10.1016/j.cobeha.2016.05.011

Naturalistic language input is associated with resting-state functional connectivity in infancy

The quantity and quality of the language input that infants receive from their caregivers affects their future language abilities; however, it is unclear how variation in this input relates to preverbal brain circuitry. The current study investigated the relation between naturalistic language input and the functional connectivity (FC) of language networks in human infancy using resting-state functional magnetic resonance imaging (rsfMRI). We recorded the naturalistic language environments of five- to eight-month-old male and female infants using the Linguistic ENvironment Analysis (LENA) system and measured the quantity and consistency of their exposure to adult words (AWs) and adult-infant conversational turns (CTs). Infants completed an rsfMRI scan during natural sleep, and we examined FC among regions of interest (ROIs) previously implicated in language comprehension, including the auditory cortex, the left inferior frontal gyrus (IFG), and the bilateral superior temporal gyrus (STG). Consistent with theory of the ontogeny of the cortical language network (Skeide and Friederici, 2016), we identified two subnetworks posited to have distinct developmental trajectories: a posterior temporal network involving connections of the auditory cortex and bilateral STG and a frontotemporal network involving connections of the left IFG. Independent of socioeconomic status (SES), the quantity of CTs was uniquely associated with FC of these networks. Infants who engaged in a larger number of CTs in daily life had lower connectivity in the posterior temporal language network. These results provide evidence for the role of vocal interactions with caregivers, compared with overheard adult speech, in the function of language networks in infancy