Acoustic treaming visualization in elastic spherical cavities

Flow visualizations are presented for acoustic streaming occurring inside spherical elastic cavities oscillating in an acoustic field. Streaming flows are visualized using Particle Image Velocimetry (PIV) and results are observed for a range of values of a dimensionless frequency parameter,M=120-306. Over the frequency range investigated, streaming flow fields remain steady at a given value ofM. The magnitude of the flows circulating inside the cavity remains small (<1 mm/s) and follows a non-linear dependency with respect to the acoustic power of the sound wave. The present boundary-driven cavity flows may enhance particle fluid transport mechanisms, leading ultimately to potential fluid mixing application

Ultra-high-speed imaging of bubbles interacting with cells and tissue

Ultrasound contrast microbubbles are exploited in molecular imaging, where bubbles are directed to target cells and where their high-scattering cross section to ultrasound allows for the detection of pathologies at a molecular level. In therapeutic applications vibrating bubbles close to cells may alter the permeability of cell membranes, and these systems are therefore highly interesting for drug and gene delivery applications using ultrasound. In a more extreme regime bubbles are driven through shock waves to sonoporate or kill cells through intense stresses or jets following inertial bubble collapse. Here, we elucidate some of the underlying mechanisms using the 25-Mfps camera Brandaris128, resolving the bubble dynamics and its interactions with cells. We quantify acoustic microstreaming around oscillating bubbles close to rigid walls and evaluate the shear stresses on nonadherent cells. In a study on the fluid dynamical interaction of cavitation bubbles with adherent cells, we find that the nonspherical collapse of bubbles is responsible for cell detachment. We also visualized the dynamics of vibrating microbubbles in contact with endothelial cells followed by fluorescent imaging of the transport of propidium iodide, used as a membrane integrity probe, into these cells showing a direct correlation between cell deformation and cell membrane permeability

Acoustic treaming visualization in elastic spherical cavities

ISSN:1343-8875ISSN:1875-897