The 'Antiretrovirals, Sexual Transmission Risk and Attitudes' (ASTRA) study. Design, methods and participant characteristics.

Life expectancy for people diagnosed with HIV has improved dramatically however the number of new infections in the UK remains high. Understanding patterns of sexual behaviour among people living with diagnosed HIV, and the factors associated with having condom-less sex, is important for informing HIV prevention strategies and clinical care. In addition, in view of the current interest in a policy of early antiretroviral treatment (ART) for all people diagnosed with HIV in the UK, it is of particular importance to assess whether ART use is associated with increased levels of condom-less sex. In this context the ASTRA study was designed to investigate current sexual activity, and attitudes to HIV transmission risk, in a large unselected sample of HIV-infected patients under care in the UK. The study also gathered background information on demographic, socio-economic, lifestyle and disease-related characteristics, and physical and psychological symptoms, in order to identify other key factors impacting on HIV patients and the behaviours which underpin transmission. In this paper we describe the study rationale, design, methods, response rate and the demographic characteristics of the participants. People diagnosed with HIV infection attending 8 UK HIV out-patient clinics in 2011-2012 were invited to participate in the study. Those who agreed to participate completed a confidential, self-administered pen-and-paper questionnaire, and their latest CD4 count and viral load test results were recorded. During the study period, 5112 eligible patients were invited to take part in the study and 3258 completed questionnaires were obtained, representing a response rate of 64% of eligible patients. The study includes 2248 men who have sex with men (MSM), 373 heterosexual men and 637 women. Future results from ASTRA will be a key resource for understanding HIV transmission within the UK, targeting prevention efforts, and informing clinical care of individuals living with HIV

Possible Disintegrating Short-Period Super-Mercury Orbiting KIC 12557548

We report here on the discovery of stellar occultations, observed with Kepler, that recur periodically at 15.685 hour intervals, but which vary in depth from a maximum of 1.3% to a minimum that can be less than 0.2%. The star that is apparently being occulted is KIC 12557548, a K dwarf with T_eff = 4400 K and V = 16. Because the eclipse depths are highly variable, they cannot be due solely to transits of a single planet with a fixed size. We discuss but dismiss a scenario involving a binary giant planet whose mutual orbit plane precesses, bringing one of the planets into and out of a grazing transit. We also briefly consider an eclipsing binary, that either orbits KIC 12557548 in a hierarchical triple configuration or is nearby on the sky, but we find such a scenario inadequate to reproduce the observations. We come down in favor of an explanation that involves macroscopic particles escaping the atmosphere of a slowly disintegrating planet not much larger than Mercury. The particles could take the form of micron-sized pyroxene or aluminum oxide dust grains. The planetary surface is hot enough to sublimate and create a high-Z atmosphere; this atmosphere may be loaded with dust via cloud condensation or explosive volcanism. Atmospheric gas escapes the planet via a Parker-type thermal wind, dragging dust grains with it. We infer a mass loss rate from the observations of order 1 M_E/Gyr, with a dust-to-gas ratio possibly of order unity. For our fiducial 0.1 M_E planet, the evaporation timescale may be ~0.2 Gyr. Smaller mass planets are disfavored because they evaporate still more quickly, as are larger mass planets because they have surface gravities too strong to sustain outflows with the requisite mass-loss rates. The occultation profile evinces an ingress-egress asymmetry that could reflect a comet-like dust tail trailing the planet; we present simulations of such a tail.Comment: 14 pages, 7 figures; submitted to ApJ, January 10, 2012; accepted March 21, 201

Joint Trajectory Generation and High-level Control for Patient-tailored Robotic Gait Rehabilitation

This dissertation presents a group of novel methods for robot-based gait rehabilitation which were developed aiming to offer more individualized therapies based on the specific condition of each patient, as well as to improve the overall rehabilitation experience for both patient and therapist. A novel methodology for gait pattern generation is proposed, which offers estimated hip and knee joint trajectories corresponding to healthy walking, and allows the therapist to graphically adapt the reference trajectories in order to fit better the patient's needs and disabilities. Additionally, the motion controllers for the hip and knee joints, mobile platform, and pelvic mechanism of an over-ground gait rehabilitation robotic system are also presented, as well as some proposed methods for assist as needed therapy. Two robot-patient synchronization approaches are also included in this work, together with a novel algorithm for online hip trajectory adaptation developed to reduce obstructive forces applied to the patient during therapy with compliant robotic systems. Finally, a prototype graphical user interface for the therapist is also presented

The contact binary VW Cephei revisited: surface activity and period variation

Context. Despite the fact that VW Cephei is one of the well-studied contact binaries in the literature, there is no fully consistent model available that can explain every observed property of this system. Aims. Our motivation is to obtain new spectra along with photometric measurements, to analyze what kind of changes may have happened in the system in the past two decades, and to propose new ideas for explaining them. Methods. For the period analysis we determined 10 new times of minima from our light curves, and constructed a new O$-$C diagram of the system. Radial velocities of the components were determined using the cross-correlation technique. The light curves and radial velocities were modelled simultaneously with the PHOEBE code. All observed spectra were compared to synthetic spectra and equivalent widths of the H$\alpha$ line were measured on their differences. Results. We have re-determined the physical parameters of the system according to our new light curve and spectral models. We confirm that the primary component is more active than the secondary, and there is a correlation between spottedness and the chromospheric activity. We propose that flip-flop phenomenon occurring on the primary component could be a possible explanation of the observed nature of the activity. To explain the period variation of VW Cep, we test two previously suggested scenarios: presence of a fourth body in the system, and the Applegate-mechanism caused by periodic magnetic activity. We conclude that although none of these mechanisms can be ruled out entirely, the available data suggest that mass transfer with a slowly decreasing rate gives the most likely explanation for the period variation of VW Cep.Comment: 13 pages, 18 figures, 9 tables, accepted for publication in Astronomy and Astrophysic

Characterization of the Humoral Immune Response during Staphylococcus aureus Bacteremia and Global Gene Expression by Staphylococcus aureus in Human Blood

Attempts to develop an efficient anti-staphylococcal vaccine in humans have so far been unsuccessful. Therefore, more knowledge of the antigens that are expressed by Staphylococcus aureus in human blood and induce an immune response in patients is required. In this study we further characterize the serial levels of IgG and IgA antibodies against 56 staphylococcal antigens in multiple serum samples of 21 patients with a S. aureus bacteremia, compare peak IgG levels between patients and 30 non-infected controls, and analyze the expression of 3626 genes by two genetically distinct isolates in human blood. The serum antibody levels were measured using a bead-based flow cytometry technique (xMAP®, Luminex corporation). Gene expression levels were analyzed using a microarray (BμG@s microarray). The initial levels and time taken to reach peak IgG and IgA antibody levels were heterogeneous in bacteremia patients. The antigen SA0688 was associated with the highest median initial-to-peak antibody fold-increase for IgG (5.05-fold) and the second highest increase for IgA (2.07-fold). Peak IgG levels against 27 antigens, including the antigen SA0688, were significantly elevated in bacteremia patients versus controls (P≤0.05). Expression of diverse genes, including SA0688, was ubiquitously high in both isolates at all time points during incubation in blood. However, only a limited number of genes were specifically up- or downregulated in both isolates when cultured in blood, compared to the start of incubation in blood or during incubation in BHI broth. In conclusion, most staphylococcal antigens tested in this study, including many known virulence factors, do not induce uniform increases in the antibody levels in bacteremia patients. In addition, the expression of these antigens by S. aureus is not significantly altered by incubation in human blood over time. One immunogenic and ubiquitously expressed antigen is the putative iron-regulated ABC transporter SA0688

A Third Hot White Dwarf Companion Detected by Kepler

We have found a system listed in the Kepler Binary Catalog (3.273 day period; Prsa et al. 2010) that we have determined is comprised of a low-mass, thermally-bloated, hot white dwarf orbiting an A star of about 2.3 solar masses. In this work we designate the object, KIC 10657664, simply as KHWD3. We use the transit depth of ~0.66%, the eclipse depth of ~1.9%, and regular smooth periodic variations at the orbital frequency and twice the orbital frequency to analyze the system parameters. The smooth periodic variations are identified with the classical ellipsoidal light variation and illumination effects, and the newly utilized Doppler boosting effect. Given the measured values of R/a and inclination angle of the binary, both the ELV and DB effects are mostly sensitive to the mass ratio, q = M_2/M_1, of the binary. The two effects yield values of q which are somewhat inconsistent - presumably due to unidentified systematic effects - but which nonetheless provide a quite useful set of possibilities for the mass of the white dwarf (either 0.18 +/- 0.03 M_Sun or 0.37 +/- 0.08 M_Sun). All of the other system parameters are determined fairly robustly. In particular, we show that the white dwarf has a radius of 0.15 +/- 0.01 R_Sun which is extremely bloated over the radius it would have as a fully degenerate object, and an effective temperature T_eff = 14,100 +/- 350 K. Binary evolution scenarios and models for this system are discussed. We suggest that the progenitor binary was comprised of a primary of mass ~2.2 M_Sun (the progenitor of the current hot white dwarf) and a secondary of mass ~1.4 M_Sun (the progenitor of the current A star in the system). We compare this new system with three other white dwarfs in binaries that likely were formed via stable Roche-lobe overflow (KOI-74, KOI-81, and Regulus).Comment: Accepted for publication in Ap