The Filament Sensor for Near Real-Time Detection of Cytoskeletal Fiber Structures

A reliable extraction of filament data from microscopic images is of high interest in the analysis of acto-myosin structures as early morphological markers in mechanically guided differentiation of human mesenchymal stem cells and the understanding of the underlying fiber arrangement processes. In this paper, we propose the filament sensor (FS), a fast and robust processing sequence which detects and records location, orientation, length and width for each single filament of an image, and thus allows for the above described analysis. The extraction of these features has previously not been possible with existing methods. We evaluate the performance of the proposed FS in terms of accuracy and speed in comparison to three existing methods with respect to their limited output. Further, we provide a benchmark dataset of real cell images along with filaments manually marked by a human expert as well as simulated benchmark images. The FS clearly outperforms existing methods in terms of computational runtime and filament extraction accuracy. The implementation of the FS and the benchmark database are available as open source.Comment: 32 pages, 21 figure

Image segmentation and feature extraction for recognizing strokes in tennis game videos

This paper addresses the problem of recognizing human actions from video. Particularly, the case of recognizing events in tennis game videos is analyzed. Driven by our domain knowledge, a robust player segmentation algorithm is developed real video data. Further, we introduce a number of novel features to be extracted for our particular application. Different feature combinations are investigated in order to find the optimal one. Finally, recognition results for different classes of tennis strokes using automatic learning capability of Hidden Markov Models (HMMs) are presented. The experimental results demonstrate that our method is close to realizing statistics of tennis games automatically using ordinary TV broadcast videos