408 research outputs found

    Research and technology

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    The research and technology accomplishments of the NASA Lewis Research Center are summarized for the fiscal year 1986, the 45th anniversary year of the Center. Five major sections are presented covering: aeronautics, aerospace technology, space communications, space station systems, and computational technology support. A table of contents by subjects was developed to assist the reader in finding articles of special interest

    Advanced Technology for Engineering Education

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    This document contains the proceedings of the Workshop on Advanced Technology for Engineering Education, held at the Peninsula Graduate Engineering Center, Hampton, Virginia, February 24-25, 1998. The workshop was jointly sponsored by the University of Virginia's Center for Advanced Computational Technology and NASA. Workshop attendees came from NASA, other government agencies, industry and universities. The objectives of the workshop were to assess the status of advanced technologies for engineering education and to explore the possibility of forming a consortium of interested individuals/universities for curriculum reform and development using advanced technologies. The presentations covered novel delivery systems and several implementations of new technologies for engineering education. Certain materials and products are identified in this publication in order to specify adequately the materials and products that were investigated in the research effort. In no case does such identification imply recommendation or endorsement of products by NASA, nor does it imply that the materials and products are the only ones or the best ones available for this purpose. In many cases equivalent materials and products are available and would probably produce equivalent results

    The Fifth Annual Thermal and Fluids Analysis Workshop

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    The Fifth Annual Thermal and Fluids Analysis Workshop was held at the Ohio Aerospace Institute, Brook Park, Ohio, cosponsored by NASA Lewis Research Center and the Ohio Aerospace Institute, 16-20 Aug. 1993. The workshop consisted of classes, vendor demonstrations, and paper sessions. The classes and vendor demonstrations provided participants with the information on widely used tools for thermal and fluid analysis. The paper sessions provided a forum for the exchange of information and ideas among thermal and fluids analysts. Paper topics included advances and uses of established thermal and fluids computer codes (such as SINDA and TRASYS) as well as unique modeling techniques and applications

    Matlab

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    This book is a collection of 19 excellent works presenting different applications of several MATLAB tools that can be used for educational, scientific and engineering purposes. Chapters include tips and tricks for programming and developing Graphical User Interfaces (GUIs), power system analysis, control systems design, system modelling and simulations, parallel processing, optimization, signal and image processing, finite different solutions, geosciences and portfolio insurance. Thus, readers from a range of professional fields will benefit from its content

    NASA Tech Briefs, February 1994

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    Topics covered include: Test and Measurement; Electronic Components and Circuits; Electronic Systems; Physical Sciences; Materials; Computer Programs; Mechanics; Machinery; Fabrication Technology; Mathematics and Information Sciences; Life Sciences; Books and Report

    Building Integrated Solar Thermal Systems. Design and Applications Handbook

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    Proceedings of the 2018 Canadian Society for Mechanical Engineering (CSME) International Congress

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    Published proceedings of the 2018 Canadian Society for Mechanical Engineering (CSME) International Congress, hosted by York University, 27-30 May 2018

    Optimization of in vivo electroporation and comparison to microinjection as delivery methods for transgenesis in zebrafish (Danio rerio). Generation of a new neuronal zebrafish line

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    Transgenic zebrafish are important models for biomedical research. There are several technologies available for the generation of transgenics and for genome editing. However, methods for the delivery of exogenous components remain limited. In Zebrafish, the most used method is microinjection, which requires sophisticated technical skills and presents a low integration rate of large constructs. Alternatively, a few studies reported the use of electroporation as a delivery method for the generation of transgenic zebrafish; however, these protocols contain some limitations that reduce their widespread applicability. To overcome this, we based on the most recent published work reporting electroporation in zebrafish embryos, to implement optimizations in order to increase the number of embryos electroporated, the efficiency of plasmid DNA delivery and its integration in the germline. Electroporation rounds of 30 one-cell stage zebrafish embryos with 300 ng/uL of plasmid DNA in PBS using 35 V poring pulse and 5 V transfer pulse yielded the highest survival and efficiency. Compared to microinjection, the optimized electroporation protocol achieved similar fluorescence intensity and expression pattern, opening the way to becoming a practical and efficient alternative to microinjection. In parallel, a new calcium indicator pan-neuronal transgenic zebrafish line, elalv3:GCaMP6fEF05 was generated, through microinjection into one-cell stage zebrafish embryos, followed by 3 rounds of fish crosses, screens, selection and raising. The improvement of delivery methods, such as electroporation, will expand the generation of new zebrafish lines for the study of developmental and molecular biology that ultimately allows the exploration of new human therapeutic avenues.Os peixes-zebra transgénicos são modelos importantes para a pesquisa biomédica. Existem várias tecnologias disponíveis para a geração de transgénicos e edição do genoma. No entanto, os métodos para a entrega de componentes exógenos permanecem limitados. No peixe-zebra, o método mais utilizado é a microinjeção, que requer habilidades técnicas sofisticadas e apresenta taxa de integração de grandes construções reduzida. Alternativamente, alguns estudos relataram o uso de eletroporação como um método de entrega para a geração de peixes-zebra transgénicos; no entanto, esses protocolos contêm algumas limitações que reduzem sua aplicabilidade generalizada. Como tal, tendo por base um trabalho publicado recentemente relatando a eletroporação de embriões de peixe-zebra, implementaram-se otimizações a fim de aumentar o número de embriões eletroporados, a eficiência da entrega de DNA plasmídico e a sua integração na linha germinativa. Ciclos de eletroporação de 30 embriões de peixe-zebra no estado de uma célula com 300 ng / uL de DNA plasmídico em PBS usando um pulso de formação de poros de 35 V e pulso de transferência de 5 V obtiveram a maior taxa de sobrevivência e eficiência. Comparado à microinjeção, o protocolo de eletroporação otimizado alcançou uma intensidade de fluorescência e padrão de expressão semelhantes, abrindo caminho para se tornar uma alternativa prática e eficiente à microinjeção. Em paralelo, uma nova linha de peixe-zebra transgénica pan-neuronal, elalv3: GCaMP6fEF05 foi gerada, através da microinjeção em embriões no estado de uma célula, seguida por 3 rondas de cruzamentos de peixes, screens, seleção e criação. A otimização dos métodos de entrega, como a eletroporação, permite expandir a geração de novas linhas de peixe-zebra para o estudo da biologia molecular e do desenvolvimento que, em última análise, permite a exploração de novos caminhos terapêuticos para humanos

    The 1992 Research/Technology report

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    The 1992 Research & Technology report is organized so that a broad cross section of the community can readily use it. A short introductory paragraph begins each article and will prove to be an invaluable reference tool for the layperson. The approximately 200 articles summarize the progress made during the year in various technical areas and portray the technical and administrative support associated with Lewis technology programs

    Proceedings of the First International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics

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    1st International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Kruger Park, 8-10 April 2002.This lecture is a principle-based review of a growing body of fundamental work stimulated by multiple opportunities to optimize geometric form (shape, structure, configuration, rhythm, topology, architecture, geography) in systems for heat and fluid flow. Currents flow against resistances, and by generating entropy (irreversibility) they force the system global performance to levels lower than the theoretical limit. The system design is destined to remain imperfect because of constraints (finite sizes, costs, times). Improvements can be achieved by properly balancing the resistances, i.e., by spreading the imperfections through the system. Optimal spreading means to endow the system with geometric form. The system construction springs out of the constrained maximization of global performance. This 'constructal' design principle is reviewed by highlighting applications from heat transfer engineering. Several examples illustrate the optimized internal structure of convection cooled packages of electronics. The origin of optimal geometric features lies in the global effort to use every volume element to the maximum, i.e., to pack the element not only with the most heat generating components, but also with the most flow, in such a way that every fluid packet is effectively engaged in cooling. In flows that connect a point to a volume or an area, the resulting structure is a tree with high conductivity branches and low-conductivity interstices.tm201
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