408 research outputs found
Research and technology
The research and technology accomplishments of the NASA Lewis Research Center are summarized for the fiscal year 1986, the 45th anniversary year of the Center. Five major sections are presented covering: aeronautics, aerospace technology, space communications, space station systems, and computational technology support. A table of contents by subjects was developed to assist the reader in finding articles of special interest
Advanced Technology for Engineering Education
This document contains the proceedings of the Workshop on Advanced Technology for Engineering Education, held at the Peninsula Graduate Engineering Center, Hampton, Virginia, February 24-25, 1998. The workshop was jointly sponsored by the University of Virginia's Center for Advanced Computational Technology and NASA. Workshop attendees came from NASA, other government agencies, industry and universities. The objectives of the workshop were to assess the status of advanced technologies for engineering education and to explore the possibility of forming a consortium of interested individuals/universities for curriculum reform and development using advanced technologies. The presentations covered novel delivery systems and several implementations of new technologies for engineering education. Certain materials and products are identified in this publication in order to specify adequately the materials and products that were investigated in the research effort. In no case does such identification imply recommendation or endorsement of products by NASA, nor does it imply that the materials and products are the only ones or the best ones available for this purpose. In many cases equivalent materials and products are available and would probably produce equivalent results
The Fifth Annual Thermal and Fluids Analysis Workshop
The Fifth Annual Thermal and Fluids Analysis Workshop was held at the Ohio Aerospace Institute, Brook Park, Ohio, cosponsored by NASA Lewis Research Center and the Ohio Aerospace Institute, 16-20 Aug. 1993. The workshop consisted of classes, vendor demonstrations, and paper sessions. The classes and vendor demonstrations provided participants with the information on widely used tools for thermal and fluid analysis. The paper sessions provided a forum for the exchange of information and ideas among thermal and fluids analysts. Paper topics included advances and uses of established thermal and fluids computer codes (such as SINDA and TRASYS) as well as unique modeling techniques and applications
Matlab
This book is a collection of 19 excellent works presenting different applications of several MATLAB tools that can be used for educational, scientific and engineering purposes. Chapters include tips and tricks for programming and developing Graphical User Interfaces (GUIs), power system analysis, control systems design, system modelling and simulations, parallel processing, optimization, signal and image processing, finite different solutions, geosciences and portfolio insurance. Thus, readers from a range of professional fields will benefit from its content
NASA Tech Briefs, February 1994
Topics covered include: Test and Measurement; Electronic Components and Circuits; Electronic Systems; Physical Sciences; Materials; Computer Programs; Mechanics; Machinery; Fabrication Technology; Mathematics and Information Sciences; Life Sciences; Books and Report
Proceedings of the 2018 Canadian Society for Mechanical Engineering (CSME) International Congress
Published proceedings of the 2018 Canadian Society for Mechanical Engineering (CSME) International Congress, hosted by York University, 27-30 May 2018
Optimization of in vivo electroporation and comparison to microinjection as delivery methods for transgenesis in zebrafish (Danio rerio). Generation of a new neuronal zebrafish line
Transgenic zebrafish are important models for biomedical research. There are several
technologies available for the generation of transgenics and for genome editing. However,
methods for the delivery of exogenous components remain limited. In Zebrafish, the most used
method is microinjection, which requires sophisticated technical skills and presents a low
integration rate of large constructs. Alternatively, a few studies reported the use of
electroporation as a delivery method for the generation of transgenic zebrafish; however, these
protocols contain some limitations that reduce their widespread applicability. To overcome
this, we based on the most recent published work reporting electroporation in zebrafish
embryos, to implement optimizations in order to increase the number of embryos
electroporated, the efficiency of plasmid DNA delivery and its integration in the germline.
Electroporation rounds of 30 one-cell stage zebrafish embryos with 300 ng/uL of plasmid DNA
in PBS using 35 V poring pulse and 5 V transfer pulse yielded the highest survival and efficiency.
Compared to microinjection, the optimized electroporation protocol achieved similar
fluorescence intensity and expression pattern, opening the way to becoming a practical and
efficient alternative to microinjection.
In parallel, a new calcium indicator pan-neuronal transgenic zebrafish line,
elalv3:GCaMP6fEF05 was generated, through microinjection into one-cell stage zebrafish
embryos, followed by 3 rounds of fish crosses, screens, selection and raising. The improvement
of delivery methods, such as electroporation, will expand the generation of new zebrafish lines
for the study of developmental and molecular biology that ultimately allows the exploration of
new human therapeutic avenues.Os peixes-zebra transgénicos são modelos importantes para a pesquisa biomédica.
Existem várias tecnologias disponíveis para a geração de transgénicos e edição do genoma.
No entanto, os métodos para a entrega de componentes exógenos permanecem limitados.
No peixe-zebra, o método mais utilizado é a microinjeção, que requer habilidades técnicas
sofisticadas e apresenta taxa de integração de grandes construções reduzida.
Alternativamente, alguns estudos relataram o uso de eletroporação como um método de
entrega para a geração de peixes-zebra transgénicos; no entanto, esses protocolos contêm
algumas limitações que reduzem sua aplicabilidade generalizada. Como tal, tendo por base
um trabalho publicado recentemente relatando a eletroporação de embriões de peixe-zebra,
implementaram-se otimizações a fim de aumentar o número de embriões eletroporados, a
eficiência da entrega de DNA plasmídico e a sua integração na linha germinativa. Ciclos de
eletroporação de 30 embriões de peixe-zebra no estado de uma célula com 300 ng / uL de
DNA plasmídico em PBS usando um pulso de formação de poros de 35 V e pulso de
transferência de 5 V obtiveram a maior taxa de sobrevivência e eficiência. Comparado à
microinjeção, o protocolo de eletroporação otimizado alcançou uma intensidade de
fluorescência e padrão de expressão semelhantes, abrindo caminho para se tornar uma
alternativa prática e eficiente à microinjeção.
Em paralelo, uma nova linha de peixe-zebra transgénica pan-neuronal, elalv3:
GCaMP6fEF05 foi gerada, através da microinjeção em embriões no estado de uma célula,
seguida por 3 rondas de cruzamentos de peixes, screens, seleção e criação. A otimização dos
métodos de entrega, como a eletroporação, permite expandir a geração de novas linhas de
peixe-zebra para o estudo da biologia molecular e do desenvolvimento que, em última análise,
permite a exploração de novos caminhos terapêuticos para humanos
The 1992 Research/Technology report
The 1992 Research & Technology report is organized so that a broad cross section of the community can readily use it. A short introductory paragraph begins each article and will prove to be an invaluable reference tool for the layperson. The approximately 200 articles summarize the progress made during the year in various technical areas and portray the technical and administrative support associated with Lewis technology programs
Proceedings of the First International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics
1st International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, Kruger Park, 8-10 April 2002.This lecture is a principle-based review of a growing body
of fundamental work stimulated by multiple opportunities to
optimize geometric form (shape, structure, configuration,
rhythm, topology, architecture, geography) in systems for heat
and fluid flow. Currents flow against resistances, and by
generating entropy (irreversibility) they force the system global
performance to levels lower than the theoretical limit. The
system design is destined to remain imperfect because of
constraints (finite sizes, costs, times). Improvements can be
achieved by properly balancing the resistances, i.e., by spreading
the imperfections through the system. Optimal spreading means
to endow the system with geometric form. The system
construction springs out of the constrained maximization of
global performance. This 'constructal' design principle is
reviewed by highlighting applications from heat transfer
engineering. Several examples illustrate the optimized internal
structure of convection cooled packages of electronics. The
origin of optimal geometric features lies in the global effort to
use every volume element to the maximum, i.e., to pack the
element not only with the most heat generating components, but
also with the most flow, in such a way that every fluid packet is
effectively engaged in cooling. In flows that connect a point to
a volume or an area, the resulting structure is a tree with high conductivity
branches and low-conductivity interstices.tm201
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