In vivo measurement of human brain elasticity using a light aspiration device

The brain deformation that occurs during neurosurgery is a serious issue impacting the patient "safety" as well as the invasiveness of the brain surgery. Model-driven compensation is a realistic and efficient solution to solve this problem. However, a vital issue is the lack of reliable and easily obtainable patient-specific mechanical characteristics of the brain which, according to clinicians' experience, can vary considerably. We designed an aspiration device that is able to meet the very rigorous sterilization and handling process imposed during surgery, and especially neurosurgery. The device, which has no electronic component, is simple, light and can be considered as an ancillary instrument. The deformation of the aspirated tissue is imaged via a mirror using an external camera. This paper describes the experimental setup as well as its use during a specific neurosurgery. The experimental data was used to calibrate a continuous model. We show that we were able to extract an in vivo constitutive law of the brain elasticity: thus for the first time, measurements are carried out per-operatively on the patient, just before the resection of the brain parenchyma. This paper discloses the results of a difficult experiment and provide for the first time in-vivo data on human brain elasticity. The results point out the softness as well as the highly non-linear behavior of the brain tissue.Comment: Medical Image Analysis (2009) accept\'

Piezoelectric needle sensor reveals mechanical heterogeneity in human thyroid tissue lesions.

Palpable thyroid lesions are common, and although mostly benign, lethal malignant nodules do occur and may be difficult to differentiate. Here, we introduce the use of a piezoelectric system called Smart-touch fine needle (or STFN) mounted directly onto conventional biopsy needles, to evaluate abnormal tissues, through quantitative real-time measurements of variations in tissue stiffness as the needle penetrates tissue. Using well-characterized biomaterials of known stiffness and explanted animal tissue models, we first established experimental protocols for STFN measures on biological tissues, as well as optimized device design for high signal-to-noise ratio. Freshly excised patient thyroids with varying fibrotic and malignant potential revealed discrete variations in STFN based tissue stiffness/stiffness heterogeneity and correlated well with final histopathology. Our piezoelectric needle sensor reveals mechanical heterogeneity in thyroid tissue lesions and provides a foundation for the design of hand-held tools for the rapid, mechano-profiling of malignant lesions in vivo while performing fine needle aspiration (FNA)

Injury Risk Estimation Expertise: Interdisciplinary Differences in Performance on the ACL Injury Risk Estimation Quiz

Background: Simple observational assessment of movement is a potentially low-cost method for anterior cruciate ligament (ACL) injury screening and prevention. Although many individuals utilize some form of observational assessment of movement, there are currently no substantial data on group skill differences in observational screening of ACL injury risk. Purpose/Hypothesis: The purpose of this study was to compare various groups’ abilities to visually assess ACL injury risk as well as the associated strategies and ACL knowledge levels. The hypothesis was that sports medicine professionals would perform better than coaches and exercise science academics/students and that these subgroups would all perform better than parents and other general population members. Study Design: Cross-sectional study; Level of evidence, 3. Methods: A total of 428 individuals, including physicians, physical therapists, athletic trainers, strength and conditioning coaches, exercise science researchers/students, athletes, parents, and members of the general public participated in the study. Participants completed the ACL Injury Risk Estimation Quiz (ACL-IQ) and answered questions related to assessment strategy and ACL knowledge. Results: Strength and conditioning coaches, athletic trainers, physical therapists, and exercise science students exhibited consistently superior ACL injury risk estimation ability (þ2 SD) as compared with sport coaches, parents of athletes, and members of the general public. The performance of a substantial number of individuals in the exercise sciences/sports medicines (approximately 40%) was similar to or exceeded clinical instrument-based biomechanical assessment methods (eg, ACL nomogram). Parents, sport coaches, and the general public had lower ACL-IQ, likely due to their lower ACL knowledge and to rating the importance of knee/thigh motion lower and weight and jump height higher. Conclusion: Substantial cross-professional/group differences in visual ACL injury risk estimation exist. The relatively profound differences in injury risk estimation accuracy and their potential implications for risk screening suggest the need for additional training and outreach

The impact of tool selection on back and wrist injury risk in tying steel reinforcement bars: a single case experiment

The paper explores the risk of work-related musculoskeletal injury in tying steel reinforcement bars. Three tools are compared to determine the extent to which ergonomic tools can reduce the risk of injury to the back and wrist in steel-tying. A whole body system of wearable sensors was used to measure biomechanical risk in tying. Three tools were assessed to determine their impact on the risk of work-related musculoskeletal injury when used at different heights. These were: a conventional pincer-cutting tool; a power-driven tying tool, and a long handled stapler tool. No tool was found to work best in all situations. The long handled stapler tool significantly reduced trunk inclination when used from ground to shoulder height, but produced higher trunk extension (backward bending) when used above shoulder height. The power tying tool did not reduce the need to bend when working at lower work heights. The power-tying tool produced significantly lower peak wrist flexion values compared to the conventional pincer-cutter tool at all work heights except overhead. The power tying tool involved significantly lower levels of wrist rotation than the conventional pincer-cutter tool at all work heights above knee level. Many assessments of ergonomic risk factors in construction rely on observational methods. The use of small, lightweight wearable sensors permits the objective measurement of biomechanical risk factors for work-related musculoskeletal injury, as well as providing objective performance data that can be used in the design and selection of task-specific tools. Our analysis of work by height also provides insight into the way in which risk factors and reduction opportunities afforded by different tools vary depending on the height at which work is to be performed

The impact of tool selection on back and wrist injury risk in tying steel reinforcement bars: a single case experiment

The paper explores the risk of work-related musculoskeletal injury in tying steel reinforcement bars. Three tools are compared to determine the extent to which ergonomic tools can reduce the risk of injury to the back and wrist in steel-tying. A whole body system of wearable sensors was used to measure biomechanical risk in tying. Three tools were assessed to determine their impact on the risk of work-related musculoskeletal injury when used at different heights. These were: a conventional pincer-cutting tool; a power-driven tying tool, and a long handled stapler tool. No tool was found to work best in all situations. The long handled stapler tool significantly reduced trunk inclination when used from ground to shoulder height, but produced higher trunk extension (backward bending) when used above shoulder height. The power tying tool did not reduce the need to bend when working at lower work heights. The power-tying tool produced significantly lower peak wrist flexion values compared to the conventional pincer-cutter tool at all work heights except overhead. The power tying tool involved significantly lower levels of wrist rotation than the conventional pincer-cutter tool at all work heights above knee level. Many assessments of ergonomic risk factors in construction rely on observational methods. The use of small, lightweight wearable sensors permits the objective measurement of biomechanical risk factors for work-related musculoskeletal injury, as well as providing objective performance data that can be used in the design and selection of task-specific tools. Our analysis of work by height also provides insight into the way in which risk factors and reduction opportunities afforded by different tools vary depending on the height at which work is to be performed

Background-deflection Brillouin microscopy reveals altered biomechanics of intracellular stress granules by ALS protein FUS

Altered cellular biomechanics have been implicated as key photogenic triggers in age-related diseases. An aberrant liquid-to-solid phase transition, observed in in vitro reconstituted droplets of FUS protein, has been recently proposed as a possible pathogenic mechanism for amyotrophic lateral sclerosis (ALS). Whether such transition occurs in cell environments is currently unknown as a consequence of the limited measuring capability of the existing techniques, which are invasive or lack of subcellular resolution. Here we developed a non-contact and label-free imaging method, named background-deflection Brillouin microscopy, to investigate the three-dimensional intracellular biomechanics at a sub-micron resolution. Our method exploits diffraction to achieve an unprecedented 10,000-fold enhancement in the spectral contrast of single-stage spectrometers, enabling, to the best of our knowledge, the first direct biomechanical analysis on intracellular stress granules containing ALS mutant FUS protein in fixed cells. Our findings provide fundamental insights on the critical aggregation step underlying the neurodegenerative ALS disease

Confined compression of collagen hydrogels

Reconstituted collagen hydrogels are often used for in vitro studies of cell-matrix interaction and as scaffolds for tissue engineering. Understanding the mechanical and transport behaviours of collagen hydrogels is therefore extremely important, albeit difficult due to their very high water content (typically > 99.5%). In the present study the mechanical behaviour of collagen hydrogels in confined compression was investigated using biphasic theory (J. Biomech. Eng. 102 (1980) 73), to ascertain whether the technique is sufficiently sensitive to determine differences in the characteristics of hydrogels of between 0.2% and 0.4% collagen. Peak stress, equilibrium stress, aggregate modulus and hydraulic permeability of the hydrogels exhibited sensitivity to collagen content, demonstrating that the technique is clearly able to discriminate between hydrogels with small differences in collagen content and may also be sensitive to factors that affect matrix remodelling. The results also offer additional insight into the deformation-dependent permeability of collagen hydrogels. This study suggests that confined compression, together with biphasic theory, is a suitable technique for assessing the mechanical properties of collagen hydrogels