Automated segmentation of tissue images for computerized IHC analysis

This paper presents two automated methods for the segmentation ofimmunohistochemical tissue images that overcome the limitations of themanual approach aswell as of the existing computerized techniques. The first independent method, based on unsupervised color clustering, recognizes automatically the target cancerous areas in the specimen and disregards the stroma; the second method, based on colors separation and morphological processing, exploits automated segmentation of the nuclear membranes of the cancerous cells. Extensive experimental results on real tissue images demonstrate the accuracy of our techniques compared to manual segmentations; additional experiments show that our techniques are more effective in immunohistochemical images than popular approaches based on supervised learning or active contours. The proposed procedure can be exploited for any applications that require tissues and cells exploration and to perform reliable and standardized measures of the activity of specific proteins involved in multi-factorial genetic pathologie

EM Algorithms for Weighted-Data Clustering with Application to Audio-Visual Scene Analysis

Data clustering has received a lot of attention and numerous methods, algorithms and software packages are available. Among these techniques, parametric finite-mixture models play a central role due to their interesting mathematical properties and to the existence of maximum-likelihood estimators based on expectation-maximization (EM). In this paper we propose a new mixture model that associates a weight with each observed point. We introduce the weighted-data Gaussian mixture and we derive two EM algorithms. The first one considers a fixed weight for each observation. The second one treats each weight as a random variable following a gamma distribution. We propose a model selection method based on a minimum message length criterion, provide a weight initialization strategy, and validate the proposed algorithms by comparing them with several state of the art parametric and non-parametric clustering techniques. We also demonstrate the effectiveness and robustness of the proposed clustering technique in the presence of heterogeneous data, namely audio-visual scene analysis.Comment: 14 pages, 4 figures, 4 table