1,148 research outputs found
Small-scale heterogeneity in sediments:experimental and modelling investigations
This thesis consists of several studies relating to small-scale heterogeneity in sediments. The principal aim was to further our understanding of processes occurring at microniches. The individual studies consist of: 1) a critical review of previous studies of microniches that used probes with high spatial resolution and modelling approaches; 2) an experimental study of analysis of oxyanions in sediment at high resolution that applied a newly developed preparation method for a combined AgI/FeOOH binding phase, to investigating processes occurring at a sulphidic microniche within a freshwater sediment; 3) analysis of the relationship between trace metal (cobalt) and iron and manganese in a marine sediment using DGT, although this is not directly related to microniches, these data are useful in modelling the release of microniche trace metal from authigenic oxides; 4) the development and application of a three-dimensional diagenetic model to investigate conceptually the geochemical behaviour of microniches under different conditions, and to interpret modelled observations in terms of data from the literature and known trace element geochemistry. The key results/conclusions from both the laboratory and modelling studies are: 1) for a freshwater sediment, depletions in anions (of P, V, As) at a microniche of elevated sulphide were observed and the behaviour of phosphate at this niche was attributed to uptake associated with elevated activity of sulphate reducing bacteria; 2) modelled scenarios, with varying microniche properties, were shown to be relevant to experimental observations reported in the literature. The preferential deposition of FeS at the edge of microniches (with lifetimes of 2.5-5 days), forming ‘crustal’ deposits was demonstrated. The modelled data indicated that microniches may be significant in terms of the formation of some trace element sulphides. This thesis also contains an assessment of the significance of microniche processes and a discussion of priorities for future work
Continuum and discrete approach in modeling biofilm development and structure: a review
The scientific community has recognized that almost 99% of the microbial life on earth is represented by biofilms. Considering the impacts of their sessile lifestyle on both natural and human activities, extensive experimental activity has been carried out to understand how biofilms grow and interact with the environment. Many mathematical models have also been developed to simulate and elucidate the main processes characterizing the biofilm growth. Two main mathematical approaches for biomass representation can be distinguished: continuum and discrete. This review is aimed at exploring the main characteristics of each approach. Continuum models can simulate the biofilm processes in a quantitative and deterministic way. However, they require a multidimensional formulation to take into account the biofilm spatial heterogeneity, which makes the models quite complicated, requiring significant computational effort. Discrete models are more recent and can represent the typical multidimensional structural heterogeneity of biofilm reflecting the experimental expectations, but they generate computational results including elements of randomness and introduce stochastic effects into the solutions
Non-lethal control of the cariogenic potential of an agent-based model for dental plaque
Dental caries or tooth decay is a prevalent global disease whose causative agent is the oral biofilm known as plaque. According to the ecological plaque hypothesis, this biofilm becomes pathogenic when external challenges drive it towards a state with a high proportion of acid-producing bacteria. Determining which factors control biofilm composition is therefore desirable when developing novel clinical treatments to combat caries, but is also challenging due to the system complexity and the existence of multiple bacterial species performing similar functions. Here we employ agent-based mathematical modelling to simulate a biofilm consisting of two competing, distinct types of bacterial populations, each parameterised by their nutrient uptake and aciduricity, periodically subjected to an acid challenge resulting from the metabolism of dietary carbohydrates. It was found that one population was progressively eliminated from the system to give either a benign or a pathogenic biofilm, with a tipping point between these two fates depending on a multiplicity of factors relating to microbial physiology and biofilm geometry. Parameter sensitivity was quantified by individually varying the model parameters against putative experimental measures, suggesting non-lethal interventions that can favourably modulate biofilm composition. We discuss how the same parameter sensitivity data can be used to guide the design of validation experiments, and argue for the benefits of in silico modelling in providing an additional predictive capability upstream from in vitro experiments
Preparation, Proximate Composition and Culinary Properties of Yellow Alkaline Noodles from Wheat and Raw/Pregelatinized Gadung (Dioscorea Hispida Dennst) Composite Flours
The steady increase of wheat flour price and noodle consumptions has driven researchers to find substitutes for wheat flour
in the noodle making process. In this work, yellow alkaline noodles were prepared from composite flours comprising wheat
and raw/pregelatinized gadung (Dioscorea hispida Dennst) flours. The purpose of this work was to investigate the effect of
composite flour compositions on the cooking properties (cooking yield, cooking loss and swelling index) of yellow alkaline
noodle. In addition, the sensory test and nutrition content of the yellow alkaline noodle were also evaluated for further
recommendation. The experimental results showed that a good quality yellow alkaline noodle can be prepared from
composite flour containing 20% w/w raw gadung flour. The cooking yield, cooking loss and swelling index of this noodle
were 10.32 g, 1.20 and 2.30, respectively. Another good quality yellow alkaline noodle can be made from composite flour
containing 40% w/w pregelatinized gadung flour. This noodle had cooking yield 8.93 g, cooking loss 1.20, and swelling
index of 1.88. The sensory evaluation suggested that although the color, aroma and firmness of the noodles were
significantly different (p ≤ 0.05) from wheat flour noodle, but their flavor remained closely similar. The nutrition content of the noodles also satisfied the Indonesian National Standard for noodle. Therefore, it can be concluded that wheat and raw/pregelatinized gadung composite flours can be used to manufacture yellow alkaline noodle with good quality and suitable for functional food
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Can an Off-Nominal Landing by an MMRTG-Powered Spacecraft Induce a Special Region on Mars When No Ice Is Present?
This work aims at addressing whether a catastrophic failure of an entry, descent, and landing event of a Multimission Radioisotope Thermoelectric Generator-based lander could embed the heat sources into the martian subsurface and create a local environment that (1) would temporarily satisfy the conditions for a martian Special Region and (2) could establish a transport mechanism through which introduced terrestrial organisms could be mobilized to naturally occurring Special Regions elsewhere on Mars. Two models were run, a primary model by researchers at the Lawrence Berkeley National Laboratory and a secondary model by researchers at the Jet Propulsion Laboratory, both of which were based on selected starting conditions for various surface composition cases that establish the worst-case scenario, including geological data collected by the Mars Science Laboratory at Gale Crater. The summary outputs of both modeling efforts showed similar results: that the introduction of the modeled heat source could temporarily create the conditions established for a Special Region, but that there would be no transport mechanism by which an introduced terrestrial microbe, even if it was active during the temporarily induced Special Region conditions, could be transported to a naturally occurring Special Region of Mars
40th Rocky Mountain Conference on Analytical Chemistry
Final program, abstracts, and information about the 40th annual meeting of the Rocky Mountain Conference on Analytical Chemistry, co-sponsored by the Colorado Section of the American Chemical Society and the Rocky Mountain Section of the Society for Applied Spectroscopy. Held in Denver, Colorado, July 25 - August 1, 1998
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