Advances in Microfluidics and Lab-on-a-Chip Technologies

Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes practical for point-of-care. It has also introduced new functionality, enhanced sensitivity, and reduced the time and cost involved in conventional molecular diagnostic techniques. This chapter reviews the application of microfluidics for molecular diagnostics methods such as nucleic acid amplification, next-generation sequencing, high resolution melting analysis, cytogenetics, protein detection and analysis, and cell sorting. We also review microfluidic sample preparation platforms applied to molecular diagnostics and targeted to sample-in, answer-out capabilities

Android-based smartphone application simulation and systematic design to reduce medication administration error in prehospital emergency care.

Since 1999 when the report To Err is Human: Building a Safer Health System was released, medical errors have come into focus (Kohn, 2000). In an effort to reduce medication administration errors in prehospital emergency care, an android-based smartphone application simulation was created. The app has components including QR barcode scanning, text to speech for medication cross-checking, weight-based medication dose calculations, and time stamped medication data wirelessly transferring to a database in real-time. Color standard identification was implemented, aiding to a designed systematic process for patient treatment to reduce medication errors. Direct observation was performed of emergency patient calls with Richmond Ambulance Authority’s providers for a preliminary assessment. Device testing was assessed with emergency medical interns and functionally tested in different light environments. Results showed how similar different pharmaceutical vendors created medication labeling and that 58.3% of medical experts would say this device served to reduce medication administration errors

Analytical techniques for multiplex analysis of protein biomarkers

Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine

Framework for the Integration of Mobile Device Features in PLM

Currently, companies have covered their business processes with stationary workstations while mobile business applications have limited relevance. Companies can cover their overall business processes more time-efficiently and cost-effectively when they integrate mobile users in workflows using mobile device features. The objective is a framework that can be used to model and control business applications for PLM processes using mobile device features to allow a totally new user experience

Simple identification tools in FishBase

Simple identification tools for fish species were included in the FishBase information system from its inception. Early tools made use of the relational model and characters like fin ray meristics. Soon pictures and drawings were added as a further help, similar to a field guide. Later came the computerization of existing dichotomous keys, again in combination with pictures and other information, and the ability to restrict possible species by country, area, or taxonomic group. Today, www.FishBase.org offers four different ways to identify species. This paper describes these tools with their advantages and disadvantages, and suggests various options for further development. It explores the possibility of a holistic and integrated computeraided strategy

PhoneGuide: Museum Guidance Supported by On-Device Object Recognition on Mobile Phones

We present PhoneGuide – an enhanced museum guidance approach that uses camera-equipped mobile phones and on-device object recognition. Our main technical achievement is a simple and light-weight object recognition approach that is realized with single-layer perceptron neuronal networks. In contrast to related systems which perform computational intensive image processing tasks on remote servers, our intention is to carry out all computations directly on the phone. This ensures little or even no network traffic and consequently decreases cost for online times. Our laboratory experiments and field surveys have shown that photographed museum exhibits can be recognized with a probability of over 90%. We have evaluated different feature sets to optimize the recognition rate and performance. Our experiments revealed that normalized color features are most effective for our method. Choosing such a feature set allows recognizing an object below one second on up-to-date phones. The amount of data that is required for differentiating 50 objects from multiple perspectives is less than 6KBytes

High-precision grasping and placing for mobile robots

This work presents a manipulation system for multiple labware in life science laboratories using the H20 mobile robots. The H20 robot is equipped with the Kinect V2 sensor to identify and estimate the position of the required labware on the workbench. The local features recognition based on SURF algorithm is used. The recognition process is performed for the labware to be grasped and for the workbench holder. Different grippers and labware containers are designed to manipulate different weights of labware and to realize a safe transportation