High-Speed, Photon Counting CCD Cameras for Astronomy

The design of electron multiplying CCD cameras require a very different approach from that appropriate for slow scan CCD operation. This paper describes the main problems in using electron multiplying CCDs for high-speed, photon counting applications in astronomy and how these may be substantially overcome. With careful design it is possible to operate the E2V Technologies L3CCDs at rates well in excess of that claimed by the manufacturer, and that levels of clock induced charge dramatically lower than those experienced with commercial cameras that need to operate at unity gain. Measurements of the performance of the E2V Technologies CCD201 operating at 26 MHz will be presented together with a guide to the effective reduction of clock induced charge levels. Examples of astronomical results obtained with our cameras are presented.Comment: 11 pages, 5 figure

Characterizing the Quantum Confined Stark Effect in Semiconductor Quantum Dots and Nanorods for Single-Molecule Electrophysiology

We optimized the performance of quantum confined Stark effect QCSE based voltage nanosensors. A high throughput approach for single particle QCSE characterization was developed and utilized to screen a library of such nanosensors. Type II ZnSe CdS seeded nanorods were found to have the best performance among the different nanosensors evaluated in this work. The degree of correlation between intensity changes and spectral changes of the excitons emission under applied field was characterized. An upper limit for the temporal response of individual ZnSe CdS nanorods to voltage modulation was characterized by high throughput, high temporal resolution intensity measurements using a novel photon counting camera. The measured 3.5 us response time is limited by the voltage modulation electronics and represents about 30 times higher bandwidth than needed for recording an action potential in a neuron.Comment: 36 pages, 6 figure

Photon Counting EMCCDs: New Opportunities for High Time Resolution Astrophysics

Electron Multiplying CCDs (EMCCDs) are used much less often than they might be because of the challenges they offer camera designers more comfortable with the design of slow-scan detector systems. However they offer an entirely new range of opportunities in astrophysical instrumentation. This paper will show some of the exciting new results obtained with these remarkable devices and talk about their potential in other areas of astrophysical application. We will then describe how they may be operated to give the very best performance at the lowest possible light levels. We will show that clock induced charge may be reduced to negligible levels and that, with care, devices may be clocked at significantly higher speeds than usually achieved. As an example of the advantages offered by these detectors we will show how a multi-detector EMCCD curvature wavefront sensor will revolutionise the sensitivity of adaptive optics instruments and been able to deliver the highest resolution images ever taken in the visible or the near infrared.Comment: 9 pages, 5 Figures; SPIE vol 8453, 201

Electron multiplying CCD – based detection in Fluorescence Correlation Spectroscopy and measurements in living zebrafish embryos

Fluorescence correlation spectroscopy (FCS) is an ultra-sensitive optical technique to investigate the dynamic properties of ensembles of single fluorescent molecules in solution. It is in particular suited for measurements in biological samples. High sensitivity is obtained by employing confocal microscopy setups with diffraction limited small detection volumes, and by using single-photon sensitive detectors, for example avalanche photo diodes (APD). However, fluorescence signal is hence typically collected from a single focus position in the sample only, and several measurements at different positions have to be performed successively. To overcome the time-consuming successive FCS measurements, we introduce electron multiplying CCD (EMCCD) camera-based spatially resolved detection for FCS. With this new detection method, multiplexed FCS measurements become feasible. Towards this goal, we perform FCS measurements with two focal volumes. As an application, we demonstrate spatial cross-correlation measurements between the two detection volumes, which allow to measure calibration-free diffusion coefficients and direction-sensitive processes like molecular flow in microfluidic channels. FCS is furthermore applied to living zebrafish embryos, to investigate the concentration gradient of the morphogen fibroblast growth factor 8 (Fgf8). It is shown by one-focus APD-based and two-focus EMCCD-based FCS, that Fgf8 propagates largely by random diffusion through the extracellular space in developing tissue. The stable concentration gradient is shown to arise from the equilibrium between a local morphogen production and the sink function of the receiving cells by receptor-mediated removal from the extracellular space. The study shows the applicability of FCS to whole model organisms. Especially in such dynamically changing systems in vivo, the perspective of fast parallel FCS measurements is of great importance. In this work, we exemplify parallel, spatially resolved FCS by utilizing an EMCCD camera. The approach, however, can be easily adapted to any other class of two-dimensional array detector. Novel generations of array detectors might become available in the near future, so that multiplexed spatial FCS could then emerge as a standard extension to classical one-focus FCS.Fluoreszenz-Korrelations-Spektroskopie (FCS) ist eine hochempfindliche optische Methode, um die dynamischen Eigenschaften eines Ensembles von einzelnen, fluoreszierenden Molekülen in Lösung zu erforschen. Sie ist insbesondere geeignet für Messungen in biologischen Proben. Die hohe Empfindlichkeit wird erreicht durch Verwendung konfokaler Mikroskop-Aufbauten mit beugungsbegrenztem Detektionsvolumen, und durch Messung der Fluoreszenz mit Einzelphotonen-empfindlichen Detektoren, zum Beispiel Avalanche-Photodioden (APD). Dadurch wird das Fluoreszenzsignal allerdings nur von einer einzelnen Fokusposition in der Probe eingesammelt, und mehrfache Messungen an verschiedenen Positionen in der Probe müssen nacheinander durchgeführt werden. Um die zeitaufwendigen, aufeinanderfolgenden FCS-Einzelmessungen zu überwinden, entwickeln wir in dieser Arbeit Elektronenvervielfachungs-CCD (EMCCD) Kamera-basierte räumlich aufgelöste Detektion für FCS. Mit dieser neuartigen Detektionsmethode werden Multiplex-FCS Messungen möglich. Darauf abzielend führen wir FCS Messungen mit zwei Detektionsvolumina durch. Als Anwendung nutzen wir die räumliche Kreuzkorrelation zwischen dem Signal beider Fokalvolumina. Sie ermöglicht die kalibrationsfreie Bestimmung von Diffusionskoeffizienten und die Messung von gerichteter Bewegung, wie zum Beispiel laminarem Fluss in mikrostrukturierten Kanälen. FCS wird darüber hinaus angewendet auf Messungen in lebenden Zebrafischembryonen, um den Konzentrationsgradienten des Morphogens Fibroblasten-Wachstumsfaktor 8 (Fgf8) zu untersuchen. Mit Hilfe von APD-basierter ein-Fokus FCS und EMCCD-basierter zwei-Fokus FCS zeigen wir, dass Fgf8 hauptsächlich frei diffffundiert im extrazellulären Raum des sich entwickelnden Embryos. Der stabile Konzentrationsgradient entsteht durch ein Gleichgewicht von lokaler Morphogenproduktion und globalem Morphogenabbau durch Rezeptor vermittelte Entfernung aus dem extrazellulären Raum. Die Studie zeigt die Anwendbarkeit von FCS in ganzen Modell-Organismen. Gerade in diesen sich dynamisch ändernden Systemen in vivo ist die Perspektive schneller, paralleler FCS-Messungen von großer Bedeutung. In dieser Arbeit wird räumlich aufgelöste FCS am Beispiel einer EMCCD Kamera durchgeführt. Die Herangehensweise ist jedoch einfach übertragbar auf jede andere Art von zwei-dimensionalem Flächendetektor. Neuartige Flächendetektoren könnten in naher Zukunft verfügbar sein. Dann könnte räumlich aufgelöste Multiplex-FCS eine standardisierte Erweiterung zur klassischen ein-Fokus FCS werden

Quantum-enhanced imaging and sensing with spatially correlated biphotons

In this thesis I discuss the experimental demonstration of quantum-enhanced imaging and sensing schemes able to surpass the performance of their classical counterparts. This is achieved by exploiting the spatial properties of quantum correlated biphotons. Over the next chapters I first discuss the production and detection of quantum correlated photons using a type-I nonlinear crystal and a single-photon sensitive electron-multiplying CCD camera. I then provide a simple yet powerful description of the spatially resolved detection of biphotons, allowing to accurately model and assess the performance of the quantum-enhanced schemes featured in this thesis. These consist of a shadow-sensing and an imaging scheme able to respectively beat the shot-noise-limit in the optical measurement of the position of a shadow and the diffraction limit in the full-field imaging of real-world objects. A combination of simulated and experimental results are used to investigate both the achieved and theoretically available quantum advantage. Optical losses and detector noise are found to limit the better-than-classical performance of the schemes, which rely on the ability to jointly detect an as high as possible number of spatially correlated biphotons

Technology advancement of the CCD201-20 EMCCD for the WFIRST coronagraph instrument: sensor characterization and radiation damage

The Wide Field InfraRed Survey Telescope-Astrophysics Focused Telescope Asset (WFIRST-AFTA) mission is a 2.4-m class space telescope that will be used across a swath of astrophysical research domains. JPL will provide a high-contrast imaging coronagraph instrument—one of two major astronomical instruments. In order to achieve the low noise performance required to detect planets under extremely low flux conditions, the electron multiplying charge-coupled device (EMCCD) has been baselined for both of the coronagraph’s sensors—the imaging camera and integral field spectrograph. JPL has established an EMCCD test laboratory in order to advance EMCCD maturity to technology readiness level-6. This plan incorporates full sensor characterization, including read noise, dark current, and clock-induced charge. In addition, by considering the unique challenges of the WFIRST space environment, degradation to the sensor’s charge transfer efficiency will be assessed, as a result of damage from high-energy particles such as protons, electrons, and cosmic rays. Science-grade CCD201-20 EMCCDs have been irradiated to a proton fluence that reflects the projected WFIRST orbit. Performance degradation due to radiation displacement damage is reported, which is the first such study for a CCD201-20 that replicates the WFIRST conditions. In addition, techniques intended to identify and mitigate radiation-induced electron trapping, such as trap pumping, custom clocking, and thermal cycling, are discussed

Towards single photon detection with a practical EMCCD

The stochastic transfer function (STF) is a metric for imaging of a microscope that includes the idea of noise, this project attempted to obtain the STF by Monte Carlo method with photon counting device. In this project, a very sensitive Electron-multiplying CCD (EMCCD) camera is evaluated for its photon counting performance on STF measurement. The evaluation system employs a beam with a very weak intensity laser to illuminate the EMCCD, the output signal and background noise of the EMCCD is recorded and statistical data is obtained. The EMCCD output signal distribution under different conditions is given by a simulation. Moreover, photon counting in TIRF with fluorescent beads is also carried out to simulate the practical STF measurement condition. Although the experiment results suggest that the EMCCD camera used in this project is not capable of performing photon counting for the Monte Carlo method, the performance of this EMCCD camera is studied in detail. The evaluation method and data can be used to evaluate other EMCCD or photon counting devices