An active curve approach for tomographic reconstruction of binary radially symmetric objects

This paper deals with a method of tomographic reconstruction of radially symmetric objects from a single radiograph, in order to study the behavior of shocked material. The usual tomographic reconstruction algorithms such as generalized inverse or filtered back-projection cannot be applied here because data are very noisy and the inverse problem associated to single view tomographic reconstruction is highly unstable. In order to improve the reconstruction, we propose here to add some a priori assumptions on the looked after object. One of these assumptions is that the object is binary and consequently, the object may be described by the curves that separate the two materials. We present a model that lives in BV space and leads to a non local Hamilton-Jacobi equation, via a level set strategy. Numerical experiments are performed (using level sets methods) on synthetic objects

Visual Quality Enhancement in Optoacoustic Tomography using Active Contour Segmentation Priors

Segmentation of biomedical images is essential for studying and characterizing anatomical structures, detection and evaluation of pathological tissues. Segmentation has been further shown to enhance the reconstruction performance in many tomographic imaging modalities by accounting for heterogeneities of the excitation field and tissue properties in the imaged region. This is particularly relevant in optoacoustic tomography, where discontinuities in the optical and acoustic tissue properties, if not properly accounted for, may result in deterioration of the imaging performance. Efficient segmentation of optoacoustic images is often hampered by the relatively low intrinsic contrast of large anatomical structures, which is further impaired by the limited angular coverage of some commonly employed tomographic imaging configurations. Herein, we analyze the performance of active contour models for boundary segmentation in cross-sectional optoacoustic tomography. The segmented mask is employed to construct a two compartment model for the acoustic and optical parameters of the imaged tissues, which is subsequently used to improve accuracy of the image reconstruction routines. The performance of the suggested segmentation and modeling approach are showcased in tissue-mimicking phantoms and small animal imaging experiments.Comment: Accepted for publication in IEEE Transactions on Medical Imagin

A Time-Evolving 3D Method Dedicated to the Reconstruction of Solar plumes and Results Using Extreme Ultra-Violet Data

An important issue in the tomographic reconstruction of the solar poles is the relatively rapid evolution of the polar plumes. We demonstrate that it is possible to take into account this temporal evolution in the reconstruction. The difficulty of this problem comes from the fact that we want a 4D reconstruction (three spatial dimensions plus time) while we only have 3D data (2D images plus time). To overcome this difficulty, we introduce a model that describes polar plumes as stationary objects whose intensity varies homogeneously with time. This assumption can be physically justified if one accepts the stability of the magnetic structure. This model leads to a bilinear inverse problem. We describe how to extend linear inversion methods to these kinds of problems. Studies of simulations show the reliability of our method. Results for SOHO/EIT data show that we are able to estimate the temporal evolution of polar plumes in order to improve the reconstruction of the solar poles from only one point of view. We expect further improvements from STEREO/EUVI data when the two probes will be separated by about 60 degrees

3D correlative single-cell imaging utilizing fluorescence and refractive index tomography

Cells alter the path of light, a fact that leads to well-known aberrations in single cell or tissue imaging. Optical diffraction tomography (ODT) measures the biophysical property that causes these aberrations, the refractive index (RI). ODT is complementary to fluorescence imaging and does not require any markers. The present study introduces RI and fluorescence tomography with optofluidic rotation (RAFTOR) of suspended cells, quantifying the intracellular RI distribution and colocalizing it with fluorescence in 3D. The technique is validated with cell phantoms and used to confirm a lower nuclear RI for HL60 cells. Furthermore, the nuclear inversion of adult mouse photoreceptor cells is observed in the RI distribution. The applications shown confirm predictions of previous studies and illustrate the potential of RAFTOR to improve our understanding of cells and tissues.Comment: 15 pages, 5 figure