Analysis of the Cell Cycle Regulated Golgi Dynamics in Mammalian Cells.

The Golgi apparatus is a membranous organelle in the cell that plays essential roles in protein and lipid trafficking, sorting, processing and modifications. Its basic structure is a stack of closely aligned flattened cisternae. In mammalian cells, dozens of Golgi stacks are often laterally linked into a ribbon-like structure. Biogenesis of the Golgi during cell division occurs through a sophisticated disassembly and reassembly process that can be divided into three distinct but cooperative steps, including the deformation and reformation of the Golgi cisternae, stacks and ribbon. This study analyzes the protein machineries that control these three steps of Golgi dynamics in the cycle of mammalian cell division, with special focuses on the Golgi stacking proteins GRASP65 that regulate Golgi stack formation, the ubiquitin E3 ligase HACE1 that regulate post-mitotic Golgi membrane fusion, and GRASP65-interacting proteins that regulate Golgi ribbon formation.PHDMolecular, Cellular and Developmental BiologyUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/97962/1/dmtang_1.pd