5 research outputs found

    Bionano-Interfaces through Peptide Design

    Get PDF
    The clinical success of restoring bone and tooth function through implants critically depends on the maintenance of an infection-free, integrated interface between the host tissue and the biomaterial surface. The surgical site infections, which are the infections within one year of surgery, occur in approximately 160,000-300,000 cases in the US annually. Antibiotics are the conventional treatment for the prevention of infections. They are becoming ineffective due to bacterial antibiotic-resistance from their wide-spread use. There is an urgent need both to combat bacterial drug resistance through new antimicrobial agents and to limit the spread of drug resistance by limiting their delivery to the implant site. This work aims to reduce surgical site infections from implants by designing of chimeric antimicrobial peptides to integrate a novel and effective delivery method. In recent years, antimicrobial peptides (AMPs) have attracted interest as natural sources for new antimicrobial agents. By being part of the immune system in all life forms, they are examples of antibacterial agents with successfully maintained efficacy across evolutionary time. Both natural and synthetic AMPs show significant promise for solving the antibiotic resistance problems. In this work, AMP1 and AMP2 was shown to be active against three different strains of pathogens in Chapter 4. In the literature, these peptides have been shown to be effective against multi-drug resistant bacteria. However, their effective delivery to the implantation site limits their clinical use. In recent years, different groups adapted covalent chemistry-based or non-specific physical adsorption methods for antimicrobial peptide coatings on implant surfaces. Many of these procedures use harsh chemical conditions requiring multiple reaction steps. Furthermore, none of these methods allow the orientation control of these molecules on the surfaces, which is an essential consideration for biomolecules. In the last few decades, solid binding peptides attracted high interest due to their material specificity and self-assembly properties. These peptides offer robust surface adsorption and assembly in diverse applications. In this work, a design method for chimeric antimicrobial peptides that can self-assemble and self-orient onto biomaterial surfaces was demonstrated. Three specific aims used to address this two-fold strategy of self-assembly and self-orientation are: 1) Develop classification and design methods using rough set theory and genetic algorithm search to customize antibacterial peptides; 2) Develop chimeric peptides by designing spacer sequences to improve the activity of antimicrobial peptides on titanium surfaces; 3) Verify the approach as an enabling technology by expanding the chimeric design approach to other biomaterials. In Aim 1, a peptide classification tool was developed because the selection of an antimicrobial peptide for an application was difficult among the thousands of peptide sequences available. A rule-based rough-set theory classification algorithm was developed to group antimicrobial peptides by chemical properties. This work is the first time that rough set theory has been applied to peptide activity analysis. The classification method on benchmark data sets resulted in low false discovery rates. The novel rough set theory method was combined with a novel genetic algorithm search, resulting in a method for customizing active antibacterial peptides using sequence-based relationships. Inspired by the fact that spacer sequences play critical roles between functional protein domains, in Aim 2, chimeric peptides were designed to combine solid binding functionality with antimicrobial functionality. To improve how these functions worked together in the same peptide sequence, new spacer sequences were engineered. The rough set theory method from Aim 1 was used to find structure-based relationships to discover new spacer sequences which improved the antimicrobial activity of the chimeric peptides. In Aim 3, the proposed approach is demonstrated as an enabling technology. In this work, calcium phosphate was tested and verified the modularity of the chimeric antimicrobial self-assembling peptide approach. Other chimeric peptides were designed for common biomaterials zirconia and urethane polymer. Finally, an antimicrobial peptide was engineered for a dental adhesive system toward applying spacer design concepts to optimize the antimicrobial activity

    Detection of HIV by using Rough Set and Homotopy Analysis Method

    Get PDF
    The significant objective of this research is to recognize how to calculate the classification process using rough set theory (RST) for the Human immunodeficiency virus infection and acquired immune deficiency syndrome (HIV & AIDS) symptoms dataset. RST has a multi-dimensional concept with multiple approaches. In this paper, our main objective is to find the symptoms of (HIV & AIDS) using basic RST and Homotopy Analysis Method (HAM) to validate our claim using statistical techniques. We prefer RST & HAM over other soft computing techniques and Mathematical Modelling as both RST and Homotopy Analysis (HAM) because RST can handle vague and imprecise data efficiently, and HAM is a suitable technique for finding analytical solutions. We have used the chi-squared test to validate our claim.&nbsp

    Finding patterns in student and medical office data using rough sets

    Get PDF
    Data have been obtained from King Khaled General Hospital in Saudi Arabia. In this project, I am trying to discover patterns in these data by using implemented algorithms in an experimental tool, called Rough Set Graphic User Interface (RSGUI). Several algorithms are available in RSGUI, each of which is based in Rough Set theory. My objective is to find short meaningful predictive rules. First, we need to find a minimum set of attributes that fully characterize the data. Some of the rules generated from this minimum set will be obvious, and therefore uninteresting. Others will be surprising, and therefore interesting. Usual measures of strength of a rule, such as length of the rule, certainty and coverage were considered. In addition, a measure of interestingness of the rules has been developed based on questionnaires administered to human subjects. There were bugs in the RSGUI java codes and one algorithm in particular, Inductive Learning Algorithm (ILA) missed some cases that were subsequently resolved in ILA2 but not updated in RSGUI. I solved the ILA issue on RSGUI. So now ILA on RSGUI is running well and gives good results for all cases encountered in the hospital administration and student records data.Master's These

    Uncertainty Management of Intelligent Feature Selection in Wireless Sensor Networks

    Get PDF
    Wireless sensor networks (WSN) are envisioned to revolutionize the paradigm of monitoring complex real-world systems at a very high resolution. However, the deployment of a large number of unattended sensor nodes in hostile environments, frequent changes of environment dynamics, and severe resource constraints pose uncertainties and limit the potential use of WSN in complex real-world applications. Although uncertainty management in Artificial Intelligence (AI) is well developed and well investigated, its implications in wireless sensor environments are inadequately addressed. This dissertation addresses uncertainty management issues of spatio-temporal patterns generated from sensor data. It provides a framework for characterizing spatio-temporal pattern in WSN. Using rough set theory and temporal reasoning a novel formalism has been developed to characterize and quantify the uncertainties in predicting spatio-temporal patterns from sensor data. This research also uncovers the trade-off among the uncertainty measures, which can be used to develop a multi-objective optimization model for real-time decision making in sensor data aggregation and samplin

    Performance analysis of multimodal biometric fusion

    Get PDF
    Biometrics is constantly evolving technology which has been widely used in many official and commercial identification applications. In fact in recent years biometric-based authentication techniques received more attention due to increased concerns in security. Most biometric systems that are currently in use typically employ a single biometric trait. Such systems are called unibiometric systems. Despite considerable advances in recent years, there are still challenges in authentication based on a single biometric trait, such as noisy data, restricted degree of freedom, intra-class variability, non-universality, spoof attack and unacceptable error rates. Some of the challenges can be handled by designing a multimodal biometric system. Multimodal biometric systems are those which utilize or are capable of utilizing, more than one physiological or behavioural characteristic for enrolment, verification, or identification. In this thesis, we propose a novel fusion approach at a hybrid level between iris and online signature traits. Online signature and iris authentication techniques have been employed in a range of biometric applications. Besides improving the accuracy, the fusion of both of the biometrics has several advantages such as increasing population coverage, deterring spoofing activities and reducing enrolment failure. In this doctoral dissertation, we make a first attempt to combine online signature and iris biometrics. We principally explore the fusion of iris and online signature biometrics and their potential application as biometric identifiers. To address this issue, investigations is carried out into the relative performance of several statistical data fusion techniques for integrating the information in both unimodal and multimodal biometrics. We compare the results of the multimodal approach with the results of the individual online signature and iris authentication approaches. This dissertation describes research into the feature and decision fusion levels in multimodal biometrics.State of Kuwait – The Public Authority of Applied Education and Trainin
    corecore