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Measuring changes in substrate utilization in the myocardium in response to fasting using hyperpolarized [1-(13)C]butyrate and [1-(13)C]pyruvate.
Cardiac dysfunction is often associated with a shift in substrate preference for ATP production. Hyperpolarized (HP) (13)C magnetic resonance spectroscopy (MRS) has the unique ability to detect real-time metabolic changes in vivo due to its high sensitivity and specificity. Here a protocol using HP [1-(13)C]pyruvate and [1-(13)C]butyrate is used to measure carbohydrate versus fatty acid metabolism in vivo. Metabolic changes in fed and fasted Sprague Dawley rats (n = 36) were studied at 9.4 T after tail vein injections. Pyruvate and butyrate competed for acetyl-CoA production, as evidenced by significant changes in [(13)C]bicarbonate (-48%), [1-(13)C]acetylcarnitine (+113%), and [5-(13)C]glutamate (-63%), following fasting. Butyrate uptake was unaffected by fasting, as indicated by [1-(13)C]butyrylcarnitine. Mitochondrial pseudoketogenesis facilitated the labeling of the ketone bodies [1-(13)C]acetoacetate and [1-(13)C]β-hydroxybutyryate, without evidence of true ketogenesis. HP [1-(13)C]acetoacetate was increased in fasting (250%) but decreased during pyruvate co-injection (-82%). Combining HP (13)C technology and co-administration of separate imaging agents enables noninvasive and simultaneous monitoring of both fatty acid and carbohydrate oxidation. This protocol illustrates a novel method for assessing metabolic flux through different enzymatic pathways simultaneously and enables mechanistic studies of the changing myocardial energetics often associated with disease
Magnetic properties of pure and Gd doped EuO probed by NMR
An Eu NMR study in the ferromagnetic phase of pure and Gd doped EuO was
performed. A complete description of the NMR lineshape of pure EuO allowed for
the influence of doping EuO with Gd impurities to be highlighted. The presence
of a temperature dependent static magnetic inhomogeneity in Gd doped EuO was
demonstrated by studying the temperature dependence of the lineshapes. The
results suggest that the inhomogeneity in 0.6% Gd doped EuO is linked to
colossal magnetoresistance. The measurement of the spin-lattice relaxation
times as a function of temperature led to the determination of the value of the
exchange integral J as a function of Gd doping. It was found that J is
temperature independent and spatially homogeneous for all the samples and that
its value increases abruptly with increasing Gd doping.Comment: 14 pages, 10 figures, to be published in Physical Review
Reply to a Comment
Reply to the Comment by F. Corberi, E. Lipiello and M. Zannetti
(cond-mat/0211609)
A Comment on Argumentation
We use the theory of defaults and their meaning of [GS16] to develop (the
outline of a) new theory of argumentation
Reply to A. Louis Comment
Reply to A. Louis Comment: Fluid-solid phase-separation in hard-sphere
mixtures is unrelated to bond-percolationComment: Reply to a comment of PRL 82 p960 To be published in PR
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