26,507 research outputs found

    Comparison of physical fitness between healthy and mild‐to‐moderate asthmatic children with exercise symptoms: A cross‐sectional study

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    .Objective Asthma is a chronic disease that may affect physical fitness, although its primary effects on exercise capacity, muscle strength, functionality and lifestyle, in children and adolescents, are still poorly understood. This study aimed to evaluate the differences in cardiorespiratory fitness, muscle strength, lifestyle, lung function, and functionality between asthmatics with exercise symptoms and healthy children. In addition, we have analyzed the association between clinical history and the presence of asthma. Study Design Cross-sectional study including 71 patients with a diagnosis of asthma and 71 healthy children and adolescents (7–17 years of age). Anthropometric data, clinical history, disease control, lifestyle (KIDMED and physical activity questionnaires), lung function (spirometry), exercise-induced bronchoconstriction test, aerobic fitness (cardiopulmonary exercise test), muscle strength and functionality (timed up and go; timed up and down stairs) were evaluated. Results Seventy-one patients with asthma (mean age 11.5 ± 2.7) and 71 healthy subjects (mean age 10.7 ± 2.5) were included. All asthmatic children had mild to moderate and stable asthma. EIB occurred in 56.3% of asthmatic children. Lung function was significantly (p < .05) lower in the asthmatic group when compared to healthy peers, as well as the cardiorespiratory fitness, muscle strength, lifestyle and functionality. Moreover, asthmatic children were more likely to have atopic dermatitis, allergic reactions, food allergies, and a family history of asthma when compared to healthy children. Conclusions Children with mild-to-moderate asthma presenting exercise symptoms show a reduction in cardiorespiratory fitness, muscle strength, lung function, functionality, and lifestyle when compared to healthy peers. The study provides data for pediatricians to support exercise practice aiming to improve prognosis and quality of life in asthmatic children.S

    Identification of Hindbrain Neural Substrates for Motor Initiation in the hatchling Xenopus laevis Tadpole

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    Animal survival profoundly depends on the ability to detect stimuli in the environment, process them and respond accordingly. In this respect, motor responses to a sensory stimulation evolved into a variety of coordinated movements, which involve the control of brain centres over spinal locomotor circuits. The hatchling Xenopus tadpole, even in its embryonic stage, is able to detect external sensory information and to swim away if the stimulus is considered noxious. To do so, the tadpole relies on well-known ascending sensory pathway, which carries the sensory information to the brain. When the stimulus is strong enough, descending interneurons are activated, leading to the excitation of spinal CPG neurons, which causes the undulatory movement of swimming. However, the activation of descending interneurons that marks the initiation of motor response appears after a long delay from the sensory stimulation. Furthermore, the long-latency response is variable in time, as observed in the slow-summating excitation measured in descending interneurons. These two features, i.e. long-latency and variability, cannot be explained by the firing time and pattern of the ascending sensory pathway of the Xenopus tadpole. Therefore, a novel neuronal population has been proposed to lie in the hindbrain of the tadpole, and being able to 'hold' the sensory information, thus accounting for the long and variable delay of swim initiation. In this work, the role of the hindbrain in the maintenance of the long and variable response to trunk skin stimulation is investigated in the Xenopustadpole at developmental stage 37/38. A multifaceted approach has been used to unravel the neuronal mechanisms underlying the delayed motor response, including behavioural experiments, electrophysiology analysis of fictive swimming, hindbrain extracellular recordings and imaging experiments. Two novel neuronal populations have been identified in the tadpole's hindbrain, which exhibit activation patterns compatible with the role of delaying the excitation of the spinal locomotor circuit. Future work on cellular properties and synaptic connections of these newly discovered populations might shed light on the mechanism of descending control active at embryonic stage. Identifying supraspinal neuronal populations in an embryonic organism could aid in understanding mechanisms of descending motor control in more complex vertebrates

    Balancing the urban stomach: public health, food selling and consumption in London, c. 1558-1640

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    Until recently, public health histories have been predominantly shaped by medical and scientific perspectives, to the neglect of their wider social, economic and political contexts. These medically-minded studies have tended to present broad, sweeping narratives of health policy's explicit successes or failures, often focusing on extraordinary periods of epidemic disease viewed from a national context. This approach is problematic, particularly in studies of public health practice prior to 1800. Before the rise of modern scientific medicine, public health policies were more often influenced by shared social, cultural, economic and religious values which favoured maintaining hierarchy, stability and concern for 'the common good'. These values have frequently been overlooked by modern researchers. This has yielded pessimistic assessments of contemporary sanitation, implying that local authorities did not care about or prioritise the health of populations. Overly medicalised perspectives have further restricted historians' investigation and use of source material, their interpretation of multifaceted and sometimes contested cultural practices such as fasting, and their examination of habitual - and not just extraordinary - health actions. These perspectives have encouraged a focus on reactive - rather than preventative - measures. This thesis contributes to a growing body of research that expands our restrictive understandings of pre-modern public health. It focuses on how public health practices were regulated, monitored and expanded in later Tudor and early Stuart London, with a particular focus on consumption and food-selling. Acknowledging the fundamental public health value of maintaining urban foodways, it investigates how contemporaries sought to manage consumption, food production waste, and vending practices in the early modern City's wards and parishes. It delineates the practical and political distinctions between food and medicine, broadly investigates the activities, reputations of and correlations between London's guild and itinerant food vendors and licensed and irregular medical practitioners, traces the directions in which different kinds of public health policy filtered up or down, and explores how policies were enacted at a national and local level. Finally, it compares and contrasts habitual and extraordinary public health regulations, with a particular focus on how perceptions of and actual food shortages, paired with the omnipresent threat of disease, impacted broader aspects of civic life

    Uso de las histonas circulantes y sus modificaciones post-traduccionales como biomarcadores en sepsis y shock séptico

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    La sepsis es una afección potencialmente mortal causada por una respuesta anormal del huésped a una infección, produciendo respuestas fisiológicas alteradas que dañan los propios tejidos del paciente y pueden provocar disfunción orgánica e incluso la muerte. Asimismo, algunos pacientes sépticos progresan a shock séptico, caracterizado por alteraciones circulatorias, celulares y metabólicas sustanciales que aumentan el riesgo de mortalidad. A pesar de que la sepsis se caracteriza por un mal funcionamiento del sistema inmunológico, lo que a su vez conduce a una respuesta inmune alterada e inmunosupresión, la alta complejidad de la fisiopatología de la sepsis requiere una mayor investigación para comprender las respuestas inmunes que ocurren durante la sepsis. Asimismo, las histonas extracelulares circulantes han ganado relevancia como mediadores citotóxicos en la sepsis, ya que actúan como patrones moleculares asociados a daño, que inducen estrés oxidativo y activan el inflamasoma NLRP3. Estos mecanismos median la activación de la piroptosis, un mecanismo de muerte celular programada que produce inflamación mediante la expresión de IL-18, IL-1β and IL-1α. Sin embargo, a pesar de la evidencia de activación del inflamasoma en las células inmunes durante la sepsis, se desconoce si las histonas extracelulares son capaces de activar los inflamasomas endoteliales y sus consecuencias. En este trabajo destacamos el papel previamente desconocido de las histonas extracelulares, mediando la activación del inflamasoma NLRP3 y la piroptosis en las células endoteliales, contribuyendo a la disfunción endotelial y la desregulación de la respuesta inmune mediada por el endotelio. Asimismo, también demostramos cómo la acetilación de histonas disminuye la activación de la piroptosis. Además, demostramos que la piroptosis se produce en pacientes con shock séptico y los niveles de histonas circulantes se correlacionan con la expresión de citoquinas proinflamatorias y citoquinas piroptóticas, la liberación de factores de adhesión endotelial y la gravedad de la enfermedad. Proponemos la piroptosis mediada por histonas como un nuevo objetivo para desarrollar intervenciones clínicas. De manera similar, hemos analizado las respuestas inmunorelacionadas que ocurren durante las primeras etapas de la sepsis con el objetivo de proporcionar nuevos datos comparando las cantidades de citoquinas, inmunomoduladores y otros mediadores endoteliales en pacientes críticamente enfermos no sépticos, sépticos y de shock séptico. Nuestro enfoque ayudará a caracterizar rápidamente las respuestas inmunes alteradas en pacientes sépticos y de shock séptico ingresados en la Unidad de Cuidados Intensivos. Finalmente analizamos el papel de la metilación del ADN en el control del sistema inmune séptico. Nuestros resultados demostraron el papel central de la metilación del ADN modulando la respuesta molecular en los pacientes de shock séptico y contribuyendo a la inmunosupresión, a través de la alteración de los patrones de metilación de los promotores de IL-10 y TREM-2.Sepsis is a life-threatening condition caused by an abnormal host response to an infection that produce altered physiological responses which damages own tissues of the patient and can result in organ dysfunction and in some cases death. Likewise, a subset of septic patients progresses to septic shock, characterized by substantial circulatory, cellular and metabolic abnormalities, which substantially increase the risk of mortality. Sepsis is characterized by a malfunction of the immune system and it can lead to an altered immune response and immunosuppression. Moreover, the high complexity of the pathophysiology of sepsis requires of further investigation to characterize the immune responses in sepsis and septic shock. Likewise, circulating extracellular histones have gained relevance as cytotoxic mediators in sepsis pathophysiology, since they act as damage-associated molecular patterns, which induce oxidative stress and activate NLRP3 inflammasome. Subsequently, inflammasome mediates pyroptosis activation, a programmed cell death mechanism that produces inflammation through the release of IL-18, IL-1β and IL-1α. However, despite inflammasome activation may occur in immune cells during sepsis, it is unknown if this process also takes place in endothelial cells and particularly whether extracellular histones are capable of activating endothelial inflammasomes and their consequences. In this work we highlight a previously unknown role for extracellular histones, that mediates the activation of NLRP3 inflammasome and pyroptosis in endothelial cells by contributing to endothelial dysfunction and the dysregulation of the immune response mediated by endothelium. Likewise, we demonstrated how histone acetylation decreases pyroptosis activation. Furthermore, we show how pyroptosis occurs in septic shock patients and how circulating histone levels correlate with the expression of pro-inflammatory and pyroptotic cytokines, the release of endothelial adhesion factors and septic shock severity. We propose histone-mediated pyroptosis as a new target to develop clinical interventions. Similarly, we have analyzed the immune-related responses occurring during the early stages of sepsis with the aim of providing new data by comparing the amounts of cytokines, immune modulators and other endothelial mediators in critically-ill non-septic patients, septic and septic shock patients. Our approach will help to rapidly characterize the altered immune responses in septic and septic shock patients admitted in the Intensive Care Unit. Finally, we also analyzed the role of DNA methylation in the control of septic immune system. Our results demonstrated the central role of DNA methylation modulating the molecular response in septic shock patients and contributing to immunosuppression, through the alteration of DNA methylation patterns of IL-10 and TREM2 promoters

    Rainfall Prediction: A Comparative Analysis of Modern Machine Learning Algorithms for Time-Series Forecasting

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    Rainfall forecasting has gained utmost research relevance in recent times due to its complexities and persistent applications such as flood forecasting and monitoring of pollutant concentration levels, among others. Existing models use complex statistical models that are often too costly, both computationally and budgetary, or are not applied to downstream applications. Therefore, approaches that use Machine Learning algorithms in conjunction with time-series data are being explored as an alternative to overcome these drawbacks. To this end, this study presents a comparative analysis using simplified rainfall estimation models based on conventional Machine Learning algorithms and Deep Learning architectures that are efficient for these downstream applications. Models based on LSTM, Stacked-LSTM, Bidirectional-LSTM Networks, XGBoost, and an ensemble of Gradient Boosting Regressor, Linear Support Vector Regression, and an Extra-trees Regressor were compared in the task of forecasting hourly rainfall volumes using time-series data. Climate data from 2000 to 2020 from five major cities in the United Kingdom were used. The evaluation metrics of Loss, Root Mean Squared Error, Mean Absolute Error, and Root Mean Squared Logarithmic Error were used to evaluate the models' performance. Results show that a Bidirectional-LSTM Network can be used as a rainfall forecast model with comparable performance to Stacked-LSTM Networks. Among all the models tested, the Stacked-LSTM Network with two hidden layers and the Bidirectional-LSTM Network performed best. This suggests that models based on LSTM-Networks with fewer hidden layers perform better for this approach; denoting its ability to be applied as an approach for budget-wise rainfall forecast applications

    The Influence of Quality on eWOM: A Digital Transformation in Hotel Management

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    612324There is no doubt that the use of Internet for purchasing products and services has constituted a crucial change in how people go about buying them. In the era of digital transformation, the possibility of accessing information provided by other users about their personal experiences has taken on more weight in the selection and buying processes. On these lines, traditional word-of-mouth (WOM) has given way to electronic word-of-mouth (eWOM), which constitutes a major social change. This behavior is particularly relevant in the services area, where potential users cannot in advance assess what is on offer. There is an abundant literature analyzing the effects of eWOM on different variables of interest in this sector. However, little is known about the factors that determine eWOM. Thus, the main objective of the present paper is to analyze the impact of two variables (objective quality and perceived quality) on eWOM. Both of them are crucial for potential customers in the process of finding hotel accommodations and they can motivate people to make such comments. The results demonstrate that these variables truly have a significant impact on whether or not users make comments on line. Moreover, it proved possible to observe certain differences according to the profile of the tourist involved and the destination where the hotel is located. In the current changing environment, this information is of great use for hotel managers in order to design strategies according to the type of guest they wish to attract.S

    Comparative analysis of the transition from early childhood education to primary education: factors affecting continuity between stages

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    p. 411-454The educational transition between early childhood education and primary education is a complex moment of change with repercussions throughout the academic life of the students. For this reason, it is important to seek continuity between both educational stages. A successful transition produces for the social, cognitive and emotional well-being of the student. The aim of this study is to find out how transition-related factors apply in ten European Union (EU) countries. The factors analyzed are the age of onset of transition, the teacher-student ratio, types of clustering, financing of the stage, responsibility, and both initial and in-service teacher training. The methodology followed in this study is documentary analysis and the main source of data search has been the European Commission's Eurydice portal. The results show differences in stage change within each country, especially in the explicit consideration of a transition period. The main conclusion is that there is a great difference between the northern and southern countries. The main differences between countries in the transitions from early childhood education to primary education in the EU are in the years of compulsory education, the teacher/student/unit ratio, the initial teacher training, and the decentralization of education.S

    Viability of patent insurance in Spain

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    M-24609-2013Since 1975 the FUNDACIÓN MAPFRE has involved itself in activities serving the general interests of society in different areas of business and culture along with activities aimed at improving the economic and social conditions of the least advantaged members and sectors of society. Within this framework, the FUNDACIÓN MAPFRE’s Institute of Insurance Science promotes and undertakes educational and research activities in the fields of insurance and risk management. In the area of education, its activities include specialized, post-graduate academic training carried out in association with the Pontifical University of Salamanca and courses and seminars for professionals held in Spain and Latin America. These activities have been expanded into other geographic regions thanks to cooperation with a series of institutions in Spain and other countries and an Internet training programme. The Institute offers grants for research in risk and insurance science and operates a specialized insurance and risk management Documentation Centre as support for its activities. The Institute routinely sponsors and draws up reports and publishes books dealing with insurance and risk management to improve our understanding of these fields. Some are intended as reference materials for those starting out in the study or practice of insurance affairs, while others are intended as information sources for undertaking research into specialized issues in greater depth. One of these activities is the publication of this volume, the outcome of research carried out by Drs. Pérez Carrillo and Cuypers in 2011 and 2012, under the guidance of José Antonio Aventín Arroyo

    New models involving quantum chemical parameters for assessing the chromatographic retention process

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    .Knowledge about the theoretical relationship between the analyte properties and the critical chromatographic parameters is mandatory for a better interpretation of the separation mechanism and a more leisurely development of quantitative studies. In a preliminary stage of this work, we introduce the Gumbel distribution, the extreme value distribution type-I widely used in other fields, as a novel tool for modelling the chromatographic peak shape. Further, we develop mathematical models to evaluate the effect of the experimental variables and various quantum parameters on the chromatographic indices, such as the retention time, capacity factor, asymmetry factor, tailing factor and number of theoretical plates. Finally, we propose a mechanistic behaviour for the chromatographic separation process based on the structure-retention relationship of fifteen selected drugs involving several molecular quantum parametersS

    Relaciones intergeneracionales: beneficios, satisfacción y limitaciones derivadas de la práctica de actividades presenciales y virtuales = Intergenerational relationships: benefits, satisfaction, and limitations derived from the practice of face-to-face and virtual activities

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    421 p.Introducción Durante las últimas décadas, el aislamiento social y la soledad entre los adultos mayores ha conducido a un mayor interés en el estudio de las relaciones intergeneracionales. Los programas intergeneracionales constituyen una gran oportunidad para que los adultos mayores conecten con personas de otras generaciones. Numerosos estudios han descrito los efectos beneficiosos de estos programas, mejorando la salud mental y física de las personas mayores y contribuyendo a una mejor formación académica y habilidades sociales en los niños. Las tecnologías digitales se han vuelto necesarias para realizar las actividades diarias de personas de todas las edades, incluyendo cada vez más a las personas mayores. Las TICs y las herramientas virtuales pueden ofrecer una gran oportunidad para que los adultos mayores conecten con personas de otras generaciones, lo que producirá beneficios en su calidad de vida y bienestar. Preguntas de investigación ¿Las intervenciones de base empírica empleadas para la evaluación de los efectos de las interacciones intergeneracionales cumplen los indicadores IBE y en ello influye el tipo de modalidad presencial, virtual o combinada? ¿La realización de actividades intergeneracionales de tipo presencial tiene un impacto positivo, en relación a sus beneficios, satisfacción y limitaciones, en todas las personas involucradas? ¿La realización de actividades intergeneracionales mediante el uso de herramientas virtuales tiene un impacto positivo, en relación a sus beneficios, satisfacción y limitaciones, en todas las personas involucradas? Objetivos Identificar los elementos relevantes que aseguran la eficacia de las intervenciones intergeneracionales tanto presenciales como virtuales. Evaluar los beneficios, la satisfacción y las limitaciones de las interacciones intergeneracionales derivadas de la realización de actividades presenciales. Analizar los beneficios, la satisfacción y las limitaciones de las interacciones intergeneracionales derivadas del uso de herramientas virtuales. Resultados Revisión sistemática (Artículo IV): Los análisis descriptivos y multivariados realizados mostraron que los programas con mayor número de controles IBE tienen mayor eficacia, independientemente del modo de intervención empleado, y que esta eficacia también está modulada por otras variables como la discapacidad de los participantes, su nivel de alfabetización o su pertenencia a una institución. Análisis de los beneficios, la satisfacción y las limitaciones derivadas de la realización de actividades intergeneracionales presenciales (Artículo VIII): El análisis de las variables sociodemográficas reveló que personas de 40 años o más, con autonomía personal, solteras o casadas, convivientes con su pareja y/u otro familiar y no jubilados realizaban actividades presenciales con personas de otra generación con una frecuencia significativamente mayor que el resto de grupos para cada variable. La mayoría de los encuestados que participaron en actividades intergeneracionales presenciales indicaron beneficios en su salud física y mental, estado de ánimo, relaciones, autodeterminación, participación social y educación académica. La mayoría de los participantes se mostraron bastante o muy satisfechos con la persona con la que realizaban este tipo de actividades, especialmente si esta persona era un amigo o un familiar cercano. Excepto los abuelos, las personas que participaron en actividades intergeneracionales presenciales y que no tenían limitaciones o discapacidades fueron las que indicaron los participantes con mayor frecuencia. Análisis de los beneficios, la satisfacción y las limitaciones derivadas de la realización de actividades intergeneracionales virtuales (Artículo IX): El análisis de los datos sociodemográficos de los encuestados mostró asociaciones significativas entre el uso de redes sociales y todas las variables analizadas, excepto su nivel de autonomía. La mayoría de los participantes que participaron en actividades intergeneracionales virtuales refirieron beneficios en su participación social, relaciones, estado de ánimo, salud mental y educación académica. Además, la mayoría de los participantes estaban bastante o muy satisfechos con la persona con la que utilizaron las herramientas virtuales, especialmente si esta persona era un amigo, su pareja, un hermano, otro familiar o un colega. Excepto los abuelos, las personas que participaron en actividades intergeneracionales virtuales y que no tenían limitaciones o discapacidades fueron las indicadas por los participantes más frecuentemente. Conclusiones El uso de herramientas digitales ofrece una gran oportunidad para el desarrollo de programas intergeneracionales con beneficios tanto para los niños y jóvenes como para los adultos mayores. Sin embargo, se ha identificado la necesidad de incrementar el número de intervenciones de tipo virtual que puedan mejorar la eficacia de las actividades realizadas y, al mismo tiempo, asegurar que se cumplan los indicadores de base empírica. Las interacciones intergeneracionales, tanto de tipo presencial como aquellas basadas en el uso de herramientas virtuales, pueden contribuir a mejorar la salud tanto física como mental, las habilidades y las relaciones sociales de todas las personas involucradas
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