4 research outputs found

    New Perspectives in the Definition/Evaluation of Seismic Hazard through Analysis of the Environmental Effects Induced by Earthquakes

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    The devastating effects caused by the recent catastrophic earthquakes that took place all over the world from Japan, New Zealand, to Chile, as well as those occurring in the Mediterranean basin, have once again shown that ground motion, although a serious source of direct damage, is not the only parameter to be considered, with most damage being the result of coseismic geological effects that are directly connected to the earthquake source or caused by ground shaking. The primary environmental effects induced by earthquakes as well as the secondary effects (sensu Environmental Seismic Intensity - ESI 2007 scale) must be considered for a more correct and complete evaluation of seismic hazards, at both regional and local scales. This Special Issue aims to collect all contributions that, using different methodologies, integrate new data produced with multi-disciplinary and innovative methods. These methodologies are essential for the identification and characterization of seismically active areas, and for the development of new hazard models, obtained using different survey techniques. The topic attracted a lot of interest, 19 peer-reviewed articles were collected; moreover, different areas of the world have been analyzed through these methodologies: Italy, USA, Spain, Australia, Ecuador, Guatemala, South Korea, Kyrgyzstan, Mongolia, Russia, China, Japan, and Nepal

    Psr1p interacts with SUN/sad1p and EB1/mal3p to establish the bipolar spindle

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    Regular Abstracts - Sunday Poster Presentations: no. 382During mitosis, interpolar microtubules from two spindle pole bodies (SPBs) interdigitate to create an antiparallel microtubule array for accommodating numerous regulatory proteins. Among these proteins, the kinesin-5 cut7p/Eg5 is the key player responsible for sliding apart antiparallel microtubules and thus helps in establishing the bipolar spindle. At the onset of mitosis, two SPBs are adjacent to one another with most microtubules running nearly parallel toward the nuclear envelope, creating an unfavorable microtubule configuration for the kinesin-5 kinesins. Therefore, how the cell organizes the antiparallel microtubule array in the first place at mitotic onset remains enigmatic. Here, we show that a novel protein psrp1p localizes to the SPB and plays a key role in organizing the antiparallel microtubule array. The absence of psr1+ leads to a transient monopolar spindle and massive chromosome loss. Further functional characterization demonstrates that psr1p is recruited to the SPB through interaction with the conserved SUN protein sad1p and that psr1p physically interacts with the conserved microtubule plus tip protein mal3p/EB1. These results suggest a model that psr1p serves as a linking protein between sad1p/SUN and mal3p/EB1 to allow microtubule plus ends to be coupled to the SPBs for organization of an antiparallel microtubule array. Thus, we conclude that psr1p is involved in organizing the antiparallel microtubule array in the first place at mitosis onset by interaction with SUN/sad1p and EB1/mal3p, thereby establishing the bipolar spindle.postprin

    Removal of antagonistic spindle forces can rescue metaphase spindle length and reduce chromosome segregation defects

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    Regular Abstracts - Tuesday Poster Presentations: no. 1925Metaphase describes a phase of mitosis where chromosomes are attached and oriented on the bipolar spindle for subsequent segregation at anaphase. In diverse cell types, the metaphase spindle is maintained at a relatively constant length. Metaphase spindle length is proposed to be regulated by a balance of pushing and pulling forces generated by distinct sets of spindle microtubules and their interactions with motors and microtubule-associated proteins (MAPs). Spindle length appears important for chromosome segregation fidelity, as cells with shorter or longer than normal metaphase spindles, generated through deletion or inhibition of individual mitotic motors or MAPs, showed chromosome segregation defects. To test the force balance model of spindle length control and its effect on chromosome segregation, we applied fast microfluidic temperature-control with live-cell imaging to monitor the effect of switching off different combinations of antagonistic forces in the fission yeast metaphase spindle. We show that spindle midzone proteins kinesin-5 cut7p and microtubule bundler ase1p contribute to outward pushing forces, and spindle kinetochore proteins kinesin-8 klp5/6p and dam1p contribute to inward pulling forces. Removing these proteins individually led to aberrant metaphase spindle length and chromosome segregation defects. Removing these proteins in antagonistic combination rescued the defective spindle length and, in some combinations, also partially rescued chromosome segregation defects. Our results stress the importance of proper chromosome-to-microtubule attachment over spindle length regulation for proper chromosome segregation.postprin
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