Two-photon imaging and analysis of neural network dynamics

The glow of a starry night sky, the smell of a freshly brewed cup of coffee or the sound of ocean waves breaking on the beach are representations of the physical world that have been created by the dynamic interactions of thousands of neurons in our brains. How the brain mediates perceptions, creates thoughts, stores memories and initiates actions remains one of the most profound puzzles in biology, if not all of science. A key to a mechanistic understanding of how the nervous system works is the ability to analyze the dynamics of neuronal networks in the living organism in the context of sensory stimulation and behaviour. Dynamic brain properties have been fairly well characterized on the microscopic level of individual neurons and on the macroscopic level of whole brain areas largely with the help of various electrophysiological techniques. However, our understanding of the mesoscopic level comprising local populations of hundreds to thousands of neurons (so called 'microcircuits') remains comparably poor. In large parts, this has been due to the technical difficulties involved in recording from large networks of neurons with single-cell spatial resolution and near- millisecond temporal resolution in the brain of living animals. In recent years, two-photon microscopy has emerged as a technique which meets many of these requirements and thus has become the method of choice for the interrogation of local neural circuits. Here, we review the state-of-research in the field of two-photon imaging of neuronal populations, covering the topics of microscope technology, suitable fluorescent indicator dyes, staining techniques, and in particular analysis techniques for extracting relevant information from the fluorescence data. We expect that functional analysis of neural networks using two-photon imaging will help to decipher fundamental operational principles of neural microcircuits.Comment: 36 pages, 4 figures, accepted for publication in Reports on Progress in Physic

A Proposal for a Three Detector Short-Baseline Neutrino Oscillation Program in the Fermilab Booster Neutrino Beam

A Short-Baseline Neutrino (SBN) physics program of three LAr-TPC detectors located along the Booster Neutrino Beam (BNB) at Fermilab is presented. This new SBN Program will deliver a rich and compelling physics opportunity, including the ability to resolve a class of experimental anomalies in neutrino physics and to perform the most sensitive search to date for sterile neutrinos at the eV mass-scale through both appearance and disappearance oscillation channels. Using data sets of 6.6e20 protons on target (P.O.T.) in the LAr1-ND and ICARUS T600 detectors plus 13.2e20 P.O.T. in the MicroBooNE detector, we estimate that a search for muon neutrino to electron neutrino appearance can be performed with ~5 sigma sensitivity for the LSND allowed (99% C.L.) parameter region. In this proposal for the SBN Program, we describe the physics analysis, the conceptual design of the LAr1-ND detector, the design and refurbishment of the T600 detector, the necessary infrastructure required to execute the program, and a possible reconfiguration of the BNB target and horn system to improve its performance for oscillation searches.Comment: 209 pages, 129 figure

Event Analysis of Pulse-reclosers in Distribution Systems Through Sparse Representation

The pulse-recloser uses pulse testing technology to verify that the line is clear of faults before initiating a reclose operation, which significantly reduces stress on the system components (e.g. substation transformers) and voltage sags on adjacent feeders. Online event analysis of pulse-reclosers are essential to increases the overall utility of the devices, especially when there are numerous devices installed throughout the distribution system. In this paper, field data recorded from several devices were analyzed to identify specific activity and fault locations. An algorithm is developed to screen the data to identify the status of each pole and to tag time windows with a possible pulse event. In the next step, selected time windows are further analyzed and classified using a sparse representation technique by solving an l1-regularized least-square problem. This classification is obtained by comparing the pulse signature with the reference dictionary to find a set that most closely matches the pulse features. This work also sheds additional light on the possibility of fault classification based on the pulse signature. Field data collected from a distribution system are used to verify the effectiveness and reliability of the proposed method.Comment: Accepted in: 19th International Conference on Intelligent System Application to Power Systems (ISAP), San Antonio, TX, 201

Applied sensor fault detection, identification and data reconstruction based on PCA and SOMNN for industrial systems

The paper presents two readily implementable approaches for Sensor Fault Detection, Identification (SFD/I) and faulted sensor data reconstruction in complex systems, in real-time. Specifically, Principal Component Analysis (PCA) and Self-Organizing Map Neural Networks (SOMNNs) are demonstrated for use on industrial turbine systems. In the first approach, Squared Prediction Error (SPE) based on the PCA residual space is used for SFD. SPE contribution plot is employed for SFI. A missing value approach from an extension of PCA is applied for faulted sensor data reconstruction. In the second approach, SFD is performed by SOMNN based Estimation Error (EE), and SFI is achieved by EE contribution plot. Data reconstruction is based on an extension of the SOMNN algorithm. The results are compared in each examining stage. The validation of both approaches is demonstrated through experimental data during the commissioning of an industrial 15MW turbine