Shelterbelt of fast growing tree species for mitigation of wind erosion and carbon sequestration in an open landscape of northeast Germany

PosterThe aim of this project (running 2010–2014) was to investigate the effects of a shelterbelt of fast-growing trees in a short rotation system on an adjacent wind-exposed field in the federal state of Brandenburg in terms of soil erosion protection, carbon sequestration in the soil and increasing landscape structuring and richness, biodiversity and microclimate. Moreover, it should be examined whether the energetic use of fast-growing trees is an economical alternative for farmers to the cultivation of annual crops, and general recommendations for practical use shall be derived from the project results. This project is financed by the Volkswagen AG. It is part of the larger framework ‘Biomasse für Sunfuel’ wherein the federal states of Lower Saxony, Hesse and Brandenburg and the Volkswagen AG join forces to achieve new knowledge for the development and introduction of synthetic biofuels. At the study site in Casekow, county Uckermark, NE Brandenburg, a short rotation coppice plantation (SRC) was established in spring 2010, dividing a 90-hectare field in north-south direction, the main wind direction being west. The shelterbelt of SRC has a width of 40 m and a length of 800 m. Different tree species and clones as well as different planting densities were considered. The aim was to manage the middle part of the shelterbelt with wider spaced poplars in a longer rotation (5–8 years), while its edges, composed of densely planted poplars and willows, should be harvested in a short rotation (3–4 years), in order to provide a continuous (but not identical) windbreak effect on the leeward adjacent arable land. The presentation will introduce and discuss results of the project e.g. biomass data from the different tree species and clones, results from wind measurements on the adjacent field and results from an ornithological investigation in the shelterbelt

Cold hardening and dehardening in Salix

The variation in cold hardiness in Salix in the autumn was investigated using clones of different geographic origins. In late growing season, the variation was small and inversely related to a phenotypic variation in potential growth rate. When growth had stopped in response to the reduction in daylength, however, large differences in cold hardiness developed. Northern/continental clones started cold hardening up to two months earlier and showed up to three times higher inherent rates of cold hardening than the southern/maritime ones. The two components of cold hardening, the timing of onset and the inherent rate, seemed to be separately inherited traits, as judged from analyses of the prodigy of a crossing between an early-and-rapidly hardening clone and a late-and-slowly hardening one. This suggests that cold hardiness can be improved without adversely affecting growth by selecting for a late onset of cold hardening combined with a rapid rate. Also, in the early stages, cold hardening was more sensitive to low, non-freezing temperatures in the southern/maritime clones than in the northern/continental ones. Cold hardening of stems in the autumn could be monitored from the accumulation of sugars, most predominantly sucrose, raffinose and stachyose. The accumulation of sucrose started already with the cessation of growth, whilst the accumulation of raffinose and stachyose started later and was stimulated by cool temperatures. Multivariate models using sugar data could explain 76% of the variation in cold hardiness in the early stages of hardening. Changes in levels of sugars and other compounds during cold hardening could be assessed non-intrusively from the visible and infrared reflectance spectra of stems. Multivariate models using spectral data could predict up to 96% of the variation in cold hardiness. This technique is expected to greatly facilitate breeding for improved cold hardiness by allowing rapid screening of large populations. The variation in cold hardiness in spring was also investigated. Loss of cold hardiness in spring was closely related to the bursting of buds. A relatively large genetic variation in the temperature requirement for bud burst was demonstrated indicating that this might be modified in sensitive clones to improve their cold hardiness in spring

Simulations of the Population of Centaurs II: Individual Objects

Detailed orbit integrations of clones of five Centaurs -- namely, 1996 AR20, 2060 Chiron, 1995 SN55, 2000 FZ53 and 2002 FY36 -- for durations of 3 Myr are presented. One of our Centaur sample starts with perihelion initially under the control of Jupiter (1996 AR20), two start under the control of Saturn (Chiron and 1995 SN55) and one each starts under the control of Uranus (2000 FZ53) and Neptune (2002 FY36) respectively. A variety of interesting pathways are illustrated with detailed examples including: capture into the Jovian Trojans, repeated bursts of short-period comet behaviour, capture into mean-motion resonances with the giant planets and into Kozai resonances, as well as traversals of the entire Solar system. For each of the Centaurs, we provide statistics on the numbers (i) ejected, (ii) showing short-period comet behaviour and (iii) becoming Earth and Mars crossing. For example, Chiron has over 60 % of its clones becoming short-period objects, whilst 1995 SN55 has over 35 %. Clones of these two Centaurs typically make numerous close approaches to Jupiter. At the other extreme, 2000 FZ53 has roughly 2 % of its clones becoming short-period objects. In our simulations, typically 20 % of the clones which become short-period comets subsequently evolve into Earth-crossers.Comment: 10 pages, in press at MNRA

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker.

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of heterologous counter-selection markers, here we explore novel DNA integration strategies that do not employ them, and instead exploit (i) activation or inactivation of genes leading to a selectable phenotype, and (ii) asymmetrical regions of homology to control the order of recombination events. We focus here on the industrial biofuel-producing bacterium Clostridium acetobutylicum, which previously lacked robust integration tools, but the approach we have developed is broadly applicable. Large sequences can be delivered in a series of steps, as we demonstrate by inserting the chromosome of phage lambda (minus a region apparently unstable in Escherichia coli in our cloning context) into the chromosome of C. acetobutylicum in three steps. This work should open the way to reliable integration of DNA including large synthetic constructs in diverse microorganisms. © 2011 The Author(s)

Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function.

A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs into the intended transcriptional unit potently stimulated reporter activity when the inserted test sequence contained a 3' splice site (ss). We show that two human sequences, originally reported to be internal ribosome entry sites and later to instead be promoters, mimic both types of element in dicistronic reporter assays by causing these cryptic readthrough transcripts to splice in patterns that allow efficient translation of the downstream cistron. Introduction of test sequences containing 3' ss into monocistronic luciferase reporter vectors widely used in the study of transcriptional regulation also created the false appearance of promoter function via the same mechanism. Across a large number of variants of these plasmids, we found a very highly significant correlation between reporter activity and levels of such spliced readthrough transcripts. Computational estimation of the frequency of cryptic 3' ss in genomic sequences suggests that misattribution of cis-regulatory function may be a common occurrence