Grating-Coupled Surface Plasmon Resonance (GC-SPR) Optimization for Phase-Interrogation Biosensing in a Microfluidic Chamber.

Surface Plasmon Resonance (SPR)-based sensors have the advantage of being label-free, enzyme-free and real-time. However, their spreading in multidisciplinary research is still mostly limited to prism-coupled devices. Plasmonic gratings, combined with a simple and cost-effective instrumentation, have been poorly developed compared to prism-coupled system mainly due to their lower sensitivity. Here we describe the optimization and signal enhancement of a sensing platform based on phase-interrogation method, which entails the exploitation of a nanostructured sensor. This technique is particularly suitable for integration of the plasmonic sensor in a lab-on-a-chip platform and can be used in a microfluidic chamber to ease the sensing procedures and limit the injected volume. The careful optimization of most suitable experimental parameters by numerical simulations leads to a 30–50% enhancement of SPR response, opening new possibilities for applications in the biomedical research field while maintaining the ease and versatility of the configuration

MULTI-MODE SELF-REFERENCING SURFACE PLASMON RESONANCE SENSORS

Surface-plasmon-resonance (SPR) sensors are widely used in biological, chemical, medical, and environmental sensing. This dissertation describes the design and development of dual-mode, self-referencing SPR sensors supporting two surface-plasmon modes (long- and short-range) which can differentiate surface binding interactions from bulk index changes at a single sensing location. Dual-mode SPR sensors have been optimized for surface limit of detection (LOD). In a wavelength interrogated optical setup, both surface plasmons are simultaneously excited at the same location and incident angle but at different wavelengths. To improve the sensor performance, a new approach to dual-mode SPR sensing is presented that offers improved differentiation between surface and bulk effects. By using an angular interrogation, both surface plasmons are simultaneously excited at the same location and wavelength but at different angles. Angular interrogation offers at least a factor of 3.6 improvement in surface and bulk cross-sensitivity compared to wavelength-interrogated dual-mode SPR sensors. Multi-mode SPR sensors supporting at least three surface-plasmon modes can differentiate a target surface effect from interfering surface effects and bulk index changes. This dissertation describes a tri-mode SPR sensor which supports three surface plasmon resonance modes at one single sensing position, where each mode is excited at a different wavelength. The tri-mode SPR sensor can successfully differentiate specific binding from the non-specific binding and bulk index changes

Surface plasmon resonance imaging for affinity-based biosensors

SPR imaging (SPRi) is at the forefront of optical label-free and real-time detection. It offers the possibility of monitoring hundreds of biological interactions simultaneously and from the binding profiles, allows the estimation of the kinetic parameters of the interactions between the immobilised probes and the ligands in solution. We review the current state of development of SPRi technology and its application including commercially available SPRi instruments. Attention is also given to surface chemistries for biochip functionalisation and suitable approaches to improve sensitivity

Polyethylene imine-based receptor immobilization for label free bioassays

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor

Recent advances in plasmonic sensor-based fiber optic probes for biological applications

Funding: This research was funded by National Natural Science Foundation of China (NSFC), grant number [61675008]. Acknowledgments: KN wishes to thank The Royal Society Kan Tong Po International Fellowship 2018 for the travel fund to visit Hong Kong Polytechnic University and Shenzhen Science and Technology Innovation Commission (Project GJHZ20180411185015272).Peer reviewedPublisher PD

Fiber Optic Sensing with Lossy Mode Resonances: Applications and Perspectives

This review focuses on the recent advances in lossy more resonance (LMR) fiber optic sensors. LMR sensors present many interesting features also in comparison with surface plasmon resonance (SPR), the most widespread resonance-based sensing platform. Two key parameters determine the performance of LMR sensors: geometrical configuration and material supporting the LMR. After reviewing those aspects and some fundamentals of the theory, the review focuses on the sensing mechanisms, mainly based on refractometry, and their possible applications. Many examples from the literature are reported ranging from electric field to pressure sensors and from gas detection to biosensors. Such vibrant activity on LMR sensors confirms the potentiality of this technology making it a very promising platform for sensor development

A Surface Plasmon Resonance Bio-Sensor based on Dual Core D-Shaped Photonic Crystal Fibre Embedded with Silver Nanowires for Multi-Sensing

K. Senthilnathan and K. Nakkeeran acknowledge the ASEM-DUO for support in the form of an International Joint Project Grant (2020 DUO-India-Scotland Professor Fellowship Award).Peer reviewedPostprin