The comet assay in animal models: From bugs to whales : (Part 2 Vertebrates)

The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies

The Promise of Health Information Technology: Ensuring that Florida's Children Benefit

Substantial policy interest in supporting the adoption of Health Information Technology (HIT) by the public and private sectors over the last 5 -- 7 years, was spurred in particular by the release of multiple Institute of Medicine reports documenting the widespread occurrence of medical errors and poor quality of care (Institute of Medicine, 1999 & 2001). However, efforts to focus on issues unique to children's health have been left out of many of initiatives. The purpose of this report is to identify strategies that can be taken by public and private entities to promote the use of HIT among providers who serve children in Florida

Final Environmental Impact Statement South Baggs Area Natural Gas Development Project Carbon County, Wyoming

The FEIS contains corrected and new material which supplements the Draft Environmental Impact Statement (DEIS) issued May 14, 1999. The FEIS and the DEIS comprise the complete document. Please refer to the DEIS for more detailed analyses and descriptions of the proposed action and alternatives

Quality assessment technique for ubiquitous software and middleware

The new paradigm of computing or information systems is ubiquitous computing systems. The technology-oriented issues of ubiquitous computing systems have made researchers pay much attention to the feasibility study of the technologies rather than building quality assurance indices or guidelines. In this context, measuring quality is the key to developing high-quality ubiquitous computing products. For this reason, various quality models have been defined, adopted and enhanced over the years, for example, the need for one recognised standard quality model (ISO/IEC 9126) is the result of a consensus for a software quality model on three levels: characteristics, sub-characteristics, and metrics. However, it is very much unlikely that this scheme will be directly applicable to ubiquitous computing environments which are considerably different to conventional software, trailing a big concern which is being given to reformulate existing methods, and especially to elaborate new assessment techniques for ubiquitous computing environments. This paper selects appropriate quality characteristics for the ubiquitous computing environment, which can be used as the quality target for both ubiquitous computing product evaluation processes ad development processes. Further, each of the quality characteristics has been expanded with evaluation questions and metrics, in some cases with measures. In addition, this quality model has been applied to the industrial setting of the ubiquitous computing environment. These have revealed that while the approach was sound, there are some parts to be more developed in the future

Siting Study for a Hydrokinetic Energy Project Located Offshore Southeastern Florida: Protocols for Survey Methodology for Offshore Marine Hydrokinetic Energy Projects

Dehlsen Associates, LLC was awarded a grant by the United States Department of Energy (DOE) Golden Field Office for a project titled “Siting Study Framework and Survey Methodology for Marine and Hydrokinetic Energy Project in Offshore Southeast Florida,” corresponding to DOE Grant Award Number DE-EE0002655 resulting from DOE funding Opportunity Announcement Number DE-FOA- 0000069 for Topic Area 2, and it is referred to herein as “the project.” The purpose of the project was to enhance the certainty of the survey requirements and regulatory review processes for the purpose of reducing the time, efforts, and costs associated with initial siting efforts of marine and hydrokinetic energy conversion facilities that may be proposed in the Atlantic Ocean offshore Southeast Florida. To secure early input from agencies, protocols were developed for collecting baseline geophysical information and benthic habitat data that can be used by project developers and regulators to make decisions early in the process of determining project location (i.e., the siting process) that avoid or minimize adverse impacts to sensitive marine benthic habitat. It is presumed that such an approach will help facilitate the licensing process for hydrokinetic and other ocean renewable energy projects within the study area and will assist in clarifying the baseline environmental data requirements described in the U.S. Department of the Interior Bureau of Ocean Energy Management, Regulation and Enforcement (formerly Minerals Management Service) final regulations on offshore renewable energy (30 Code of Federal Regulations 285, published April 29, 2009). Because projects generally seek to avoid or minimize impacts to sensitive marine habitats, it was not the intent of this project to investigate areas that did not appear suitable for the siting of ocean renewable energy projects. Rather, a two-tiered approach was designed with the first step consisting of gaining overall insight about seabed conditions offshore southeastern Florida by conducting a geophysical survey of pre-selected areas with subsequent post-processing and expert data interpretation by geophysicists and experienced marine biologists knowledgeable about the general project area. The second step sought to validate the benthic habitat types interpreted from the geophysical data by conducting benthic video and photographic field surveys of selected habitat types. The goal of this step was to determine the degree of correlation between the habitat types interpreted from the geophysical data and what actually exists on the seafloor based on the benthic video survey logs. This step included spot-checking selected habitat types rather than comprehensive evaluation of the entire area covered by the geophysical survey. It is important to note that non-invasive survey methods were used as part of this study and no devices of any kind were either temporarily or permanently attached to the seabed as part of the work conducted under this project

Enhancing Cyber-Resiliency of DER-based SmartGrid: A Survey

The rapid development of information and communications technology has enabled the use of digital-controlled and software-driven distributed energy resources (DERs) to improve the flexibility and efficiency of power supply, and support grid operations. However, this evolution also exposes geographically-dispersed DERs to cyber threats, including hardware and software vulnerabilities, communication issues, and personnel errors, etc. Therefore, enhancing the cyber-resiliency of DER-based smart grid - the ability to survive successful cyber intrusions - is becoming increasingly vital and has garnered significant attention from both industry and academia. In this survey, we aim to provide a systematical and comprehensive review regarding the cyber-resiliency enhancement (CRE) of DER-based smart grid. Firstly, an integrated threat modeling method is tailored for the hierarchical DER-based smart grid with special emphasis on vulnerability identification and impact analysis. Then, the defense-in-depth strategies encompassing prevention, detection, mitigation, and recovery are comprehensively surveyed, systematically classified, and rigorously compared. A CRE framework is subsequently proposed to incorporate the five key resiliency enablers. Finally, challenges and future directions are discussed in details. The overall aim of this survey is to demonstrate the development trend of CRE methods and motivate further efforts to improve the cyber-resiliency of DER-based smart grid.Comment: Submitted to IEEE Transactions on Smart Grid for Publication Consideratio

Agile Processes in Software Engineering and Extreme Programming

This open access book constitutes the proceedings of the 23rd International Conference on Agile Software Development, XP 2022, which was held in Copenhagen, Denmark, in June 2022. XP is the premier agile software development conference combining research and practice. It is a unique forum where agile researchers, practitioners, thought leaders, coaches, and trainers get together to present and discuss their most recent innovations, research results, experiences, concerns, challenges, and trends.  XP conferences provide an informal environment to learn and trigger discussions and welcome both people new to agile and seasoned agile practitioners. This year’s conference was held with the theme “Agile in the Era of Hybrid Work”. The 13 full papers and 1 short paper presented in this volume were carefully reviewed and selected from 40 submissions. They were organized in topical sections named: agile practices; agile processes; and agile in the large