1,952 research outputs found

    Registration of Standardized Histological Images in Feature Space

    Full text link
    In this paper, we propose three novel and important methods for the registration of histological images for 3D reconstruction. First, possible intensity variations and nonstandardness in images are corrected by an intensity standardization process which maps the image scale into a standard scale where the similar intensities correspond to similar tissues meaning. Second, 2D histological images are mapped into a feature space where continuous variables are used as high confidence image features for accurate registration. Third, we propose an automatic best reference slice selection algorithm that improves reconstruction quality based on both image entropy and mean square error of the registration process. We demonstrate that the choice of reference slice has a significant impact on registration error, standardization, feature space and entropy information. After 2D histological slices are registered through an affine transformation with respect to an automatically chosen reference, the 3D volume is reconstructed by co-registering 2D slices elastically.Comment: SPIE Medical Imaging 2008 - submissio

    Histopathological image analysis : a review

    Get PDF
    Over the past decade, dramatic increases in computational power and improvement in image analysis algorithms have allowed the development of powerful computer-assisted analytical approaches to radiological data. With the recent advent of whole slide digital scanners, tissue histopathology slides can now be digitized and stored in digital image form. Consequently, digitized tissue histopathology has now become amenable to the application of computerized image analysis and machine learning techniques. Analogous to the role of computer-assisted diagnosis (CAD) algorithms in medical imaging to complement the opinion of a radiologist, CAD algorithms have begun to be developed for disease detection, diagnosis, and prognosis prediction to complement the opinion of the pathologist. In this paper, we review the recent state of the art CAD technology for digitized histopathology. This paper also briefly describes the development and application of novel image analysis technology for a few specific histopathology related problems being pursued in the United States and Europe

    Automated Segmentation of Cerebral Aneurysm Using a Novel Statistical Multiresolution Approach

    Get PDF
    Cerebral Aneurysm (CA) is a vascular disease that threatens the lives of many adults. It a ects almost 1:5 - 5% of the general population. Sub- Arachnoid Hemorrhage (SAH), resulted by a ruptured CA, has high rates of morbidity and mortality. Therefore, radiologists aim to detect it and diagnose it at an early stage, by analyzing the medical images, to prevent or reduce its damages. The analysis process is traditionally done manually. However, with the emerging of the technology, Computer-Aided Diagnosis (CAD) algorithms are adopted in the clinics to overcome the traditional process disadvantages, as the dependency of the radiologist's experience, the inter and intra observation variability, the increase in the probability of error which increases consequently with the growing number of medical images to be analyzed, and the artifacts added by the medical images' acquisition methods (i.e., MRA, CTA, PET, RA, etc.) which impedes the radiologist' s work. Due to the aforementioned reasons, many research works propose di erent segmentation approaches to automate the analysis process of detecting a CA using complementary segmentation techniques; but due to the challenging task of developing a robust reproducible reliable algorithm to detect CA regardless of its shape, size, and location from a variety of the acquisition methods, a diversity of proposed and developed approaches exist which still su er from some limitations. This thesis aims to contribute in this research area by adopting two promising techniques based on the multiresolution and statistical approaches in the Two-Dimensional (2D) domain. The rst technique is the Contourlet Transform (CT), which empowers the segmentation by extracting features not apparent in the normal image scale. While the second technique is the Hidden Markov Random Field model with Expectation Maximization (HMRF-EM), which segments the image based on the relationship of the neighboring pixels in the contourlet domain. The developed algorithm reveals promising results on the four tested Three- Dimensional Rotational Angiography (3D RA) datasets, where an objective and a subjective evaluation are carried out. For the objective evaluation, six performance metrics are adopted which are: accuracy, Dice Similarity Index (DSI), False Positive Ratio (FPR), False Negative Ratio (FNR), speci city, and sensitivity. As for the subjective evaluation, one expert and four observers with some medical background are involved to assess the segmentation visually. Both evaluations compare the segmented volumes against the ground truth data
    • …
    corecore