Introducing distributed dynamic data-intensive (D3) science: Understanding applications and infrastructure

A common feature across many science and engineering applications is the amount and diversity of data and computation that must be integrated to yield insights. Data sets are growing larger and becoming distributed; and their location, availability and properties are often time-dependent. Collectively, these characteristics give rise to dynamic distributed data-intensive applications. While "static" data applications have received significant attention, the characteristics, requirements, and software systems for the analysis of large volumes of dynamic, distributed data, and data-intensive applications have received relatively less attention. This paper surveys several representative dynamic distributed data-intensive application scenarios, provides a common conceptual framework to understand them, and examines the infrastructure used in support of applications.Comment: 38 pages, 2 figure

Cloud Computing for Next-Generation Sequencing Data Analysis

High-throughput next-generation sequencing (NGS) technologies have evolved rapidly and are reshaping the scope of genomics research. The substantial decrease in the cost of NGS techniques in the past decade has led to its rapid adoption in biological research and drug development. Genomics studies of large populations are producing a huge amount of data, giving rise to computational issues around the storage, transfer, and analysis of the data. Fortunately, cloud computing has recently emerged as a viable option to quickly and easily acquire the computational resources for large-scale NGS data analyses. Some cloud-based applications and resources have been developed specifically to address the computational challenges of working with very large volumes of data generated by NGS technology. In this chapter, we will review some cloud-based systems and solutions for NGS data analysis, discuss the practical hurdles and limitations in cloud computing, including data transfer and security, and share the lessons we learned from the implementation of Rainbow, a cloud-based tool for large-scale genome sequencing data analysis

An extensible and scalable Pilot-MapReduce framework for data intensive applications on distributed cyberinfrastructure

The volume and complexity of data that must be analyzed in scientific applications is increasing exponentially. Often, this data is distributed; thus, the ability to analyze data by localizing it will yield limited returns. Therefore, an efficient processing of large distributed datasets is required, whilst ideally not introducing fundamentally new programming models or methods. For example, extending MapReduce - a proven effective programming model for processing large datasets, to work more effectively on distributed data and on different infrastructure (such as non-Hadoop, general-purpose clusters) is desirable. We posit that this can be achieved with an effective and efficient runtime environment and without refactoring MapReduce itself. MapReduce on distributed data requires effective distributed coordination of computation (map and reduce) and data, as well as distributed data management (in particular the transfer of intermediate data units). To address these requirements, we design and implement Pilot-MapReduce (PMR) - a flexible, infrastructure-independent runtime environment for MapReduce. PMR is based on Pilot abstractions for both compute (Pilot- Jobs) and data (Pilot-Data): it utilizes Pilot-Jobs to couple the map phase computation to the nearby source data, and Pilot-Data to move intermediate data using parallel data transfers to the reduce computation phase. We analyze the effectiveness of PMR over applications with different characteristics (e. g. different volumes of intermediate and output data). Our experimental evaluations show that the Pilot abstraction for data movement across multiple clusters is promising, and can lower the execution time span of the entire MapReduce execution. We also investigate the performance of PMR with distributed data using a Word Count and a genome sequencing application over different MapReduce configurations. We find that PMR is a viable tool to support distributed NGS analytics by comparing and contrasting the PMR approach to similar capabilities of Seqal and Crossbow, two Next Generation Sequencing(NGS) Hadoop MapReduce based applications. Our experiments show that PMR provides the desired flexibility in the deployment and configuration of MapReduce runs to address specific application characteristics and achieve an optimal performance, both locally and over wide-area multiple clusters

Report of the user requirements and web based access for eResearch workshops

The User Requirements and Web Based Access for eResearch Workshop, organized jointly by NeSC and NCeSS, was held on 19 May 2006. The aim was to identify lessons learned from e-Science projects that would contribute to our capacity to make Grid infrastructures and tools usable and accessible for diverse user communities. Its focus was on providing an opportunity for a pragmatic discussion between e-Science end users and tool builders in order to understand usability challenges, technological options, community-specific content and needs, and methodologies for design and development. We invited members of six UK e-Science projects and one US project, trying as far as possible to pair a user and developer from each project in order to discuss their contrasting perspectives and experiences. Three breakout group sessions covered the topics of user-developer relations, commodification, and functionality. There was also extensive post-meeting discussion, summarized here. Additional information on the workshop, including the agenda, participant list, and talk slides, can be found online at http://www.nesc.ac.uk/esi/events/685/ Reference: NeSC report UKeS-2006-07 available from http://www.nesc.ac.uk/technical_papers/UKeS-2006-07.pd

HSRA: Hadoop-based spliced read aligner for RNA sequencing data

[Abstract] Nowadays, the analysis of transcriptome sequencing (RNA-seq) data has become the standard method for quantifying the levels of gene expression. In RNA-seq experiments, the mapping of short reads to a reference genome or transcriptome is considered a crucial step that remains as one of the most time-consuming. With the steady development of Next Generation Sequencing (NGS) technologies, unprecedented amounts of genomic data introduce significant challenges in terms of storage, processing and downstream analysis. As cost and throughput continue to improve, there is a growing need for new software solutions that minimize the impact of increasing data volume on RNA read alignment. In this work we introduce HSRA, a Big Data tool that takes advantage of the MapReduce programming model to extend the multithreading capabilities of a state-of-the-art spliced read aligner for RNA-seq data (HISAT2) to distributed memory systems such as multi-core clusters or cloud platforms. HSRA has been built upon the Hadoop MapReduce framework and supports both single- and paired-end reads from FASTQ/FASTA datasets, providing output alignments in SAM format. The design of HSRA has been carefully optimized to avoid the main limitations and major causes of inefficiency found in previous Big Data mapping tools, which cannot fully exploit the raw performance of the underlying aligner. On a 16-node multi-core cluster, HSRA is on average 2.3 times faster than previous Hadoop-based tools. Source code in Java as well as a user’s guide are publicly available for download at http://hsra.dec.udc.es.Ministerio de Economía, Industria y Competitividad; TIN2016-75845-PXunta de Galicia; ED431G/0

Complex Adaptive Systems Drive Innovations in Synthetic Biology

AbstractThe resultant outcome of synthetic biology research offers the promise of changing the way that we create energy, produce food, optimize industrial processing, and detect, prevent, and cure diseases. Researchers can now realize the significant benefits of the cloud computing model to reduce IT cost and complexity, enhance R&D collaboration, and improve bioinformatics to allow lifesciences organizations to focus on answering some of lifes most challenging questions