Precision Pointing Control System (PPCS) system design and analysis

The precision pointing control system (PPCS) is an integrated system for precision attitude determination and orientation of gimbaled experiment platforms. The PPCS concept configures the system to perform orientation of up to six independent gimbaled experiment platforms to design goal accuracy of 0.001 degrees, and to operate in conjunction with a three-axis stabilized earth-oriented spacecraft in orbits ranging from low altitude (200-2500 n.m., sun synchronous) to 24 hour geosynchronous, with a design goal life of 3 to 5 years. The system comprises two complementary functions: (1) attitude determination where the attitude of a defined set of body-fixed reference axes is determined relative to a known set of reference axes fixed in inertial space; and (2) pointing control where gimbal orientation is controlled, open-loop (without use of payload error/feedback) with respect to a defined set of body-fixed reference axes to produce pointing to a desired target

On non-Gaussian beams and optomechanical parametric instabilities in interferometric gravitational wave detectors

Direct detection of gravitational radiation, predicted by Einstein’s general theory of relativity, remains one of the most exciting challenges in experimental physics. Due to their relatively weak interaction with matter, gravitational waves promise to allow exploration of hitherto inaccessible objects and epochs. Unfortunately, this weak coupling also hinders detection with strain amplitudes at the Earth estimated to be of order 10^−21. Due to their wide bandwidth and theoretical sensitivity, kilometre-scale Michelson style interferometers have become the preferred instrument with which to attempt ground based detection. A worldwide network of first generation instruments has been constructed and prodigious volumes of data recorded. Despite each instrument approaching or having reached its design sensitivity, a confirmed detection remains elusive. Planned upgrades to these instruments aim to increase strain sensitivity by an order of magnitude, commencing the era of second generation detectors. Entry into this regime will be accompanied by an entirely new set of challenges, two of which are addressed in this work. As advanced interferometers are commissioned, instrumental artifacts will give way to fundamental noise sources. In the region of peak sensitivity it is expected that thermal noise in the interferometers’ dielectric mirror coatings will be the principal source of displacement noise. Theory suggests that increasing the spot size of laser light incident on these mirrors will reduce the measured thermal noise. In the first part of this work we examine one method of realising larger spots. By adopting non-spherical mirrors in the interferometers’ arms it is possible to create resonators which support a wide, flat-topped field known as the mesa beam. This beam has been shown to theoretically reduce all forms of mirror thermal noise without being significantly more difficult to control. In this work we investigate these claims using a bespoke prototype mirror. The first results regarding a non-Gaussian beam created in a manner applicable to a gravitational wave interferometer are presented. A common theme among all second generation interferometer designs is the desire to maximise circulating power. This increased power is partnered by commensurately increased thermal perturbations. Since the attractive properties of the mesa beam are produced by the fine structure of its supporting mirrors, it is important that we understand the effects absorption of stored optical power could have on mesa fields. In the second part of this work we report on numerical evaluations of measured thermal noise and mesa beam intensity profile as a function of absorbed power. Increased optical power also has less obvious consequences. As a result of radiation pressure, there exists a pathway between optical energy stored in an interferometer’s arms and mechanical energy stored in the acoustic modes of its test masses. Under appropriate conditions, this coupling can excite one or more test masses to such a degree that interferometer operation becomes impossible. In the final part of this work we determine whether it is possible to mitigate these parametric instabilities using electrostatic actuators originally designed to control the position and orientation of the test masses

Biological application of Compressed Sensing Tomography in the Scanning Electron Microscope

The three-dimensional tomographic reconstruction of a biological sample, namely collagen fibrils in human dermal tissue, was obtained from a set of projection-images acquired in the Scanning Electron Microscope. A tailored strategy for the transmission imaging mode was implemented in the microscope and proved effective in acquiring the projections needed for the tomographic reconstruction. Suitable projection alignment and Compressed Sensing formulation were used to overcome the limitations arising from the experimental acquisition strategy and to improve the reconstruction of the sample. The undetermined problem of structure reconstruction from a set of projections, limited in number and angular range, was indeed supported by exploiting the sparsity of the object projected in the electron microscopy images. In particular, the proposed system was able to preserve the reconstruction accuracy even in presence of a significant reduction of experimental projections

Optical In-Process Measurement Systems

Information is key, which means that measurements are key. For this reason, this book provides unique insight into state-of-the-art research works regarding optical measurement systems. Optical systems are fast and precise, and the ongoing challenge is to enable optical principles for in-process measurements. Presented within this book is a selection of promising optical measurement approaches for real-world applications

Validação de heterogeneidade estrutural em dados de Crio-ME por comitês de agrupadores

Orientadores: Fernando José Von Zuben, Rodrigo Villares PortugalDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Elétrica e de ComputaçãoResumo: Análise de Partículas Isoladas é uma técnica que permite o estudo da estrutura tridimensional de proteínas e outros complexos macromoleculares de interesse biológico. Seus dados primários consistem em imagens de microscopia eletrônica de transmissão de múltiplas cópias da molécula em orientações aleatórias. Tais imagens são bastante ruidosas devido à baixa dose de elétrons utilizada. Reconstruções 3D podem ser obtidas combinando-se muitas imagens de partículas em orientações similares e estimando seus ângulos relativos. Entretanto, estados conformacionais heterogêneos frequentemente coexistem na amostra, porque os complexos moleculares podem ser flexíveis e também interagir com outras partículas. Heterogeneidade representa um desafio na reconstrução de modelos 3D confiáveis e degrada a resolução dos mesmos. Entre os algoritmos mais populares usados para classificação estrutural estão o agrupamento por k-médias, agrupamento hierárquico, mapas autoorganizáveis e estimadores de máxima verossimilhança. Tais abordagens estão geralmente entrelaçadas à reconstrução dos modelos 3D. No entanto, trabalhos recentes indicam ser possível inferir informações a respeito da estrutura das moléculas diretamente do conjunto de projeções 2D. Dentre estas descobertas, está a relação entre a variabilidade estrutural e manifolds em um espaço de atributos multidimensional. Esta dissertação investiga se um comitê de algoritmos de não-supervisionados é capaz de separar tais "manifolds conformacionais". Métodos de "consenso" tendem a fornecer classificação mais precisa e podem alcançar performance satisfatória em uma ampla gama de conjuntos de dados, se comparados a algoritmos individuais. Nós investigamos o comportamento de seis algoritmos de agrupamento, tanto individualmente quanto combinados em comitês, para a tarefa de classificação de heterogeneidade conformacional. A abordagem proposta foi testada em conjuntos sintéticos e reais contendo misturas de imagens de projeção da proteína Mm-cpn nos estados "aberto" e "fechado". Demonstra-se que comitês de agrupadores podem fornecer informações úteis na validação de particionamentos estruturais independetemente de algoritmos de reconstrução 3DAbstract: Single Particle Analysis is a technique that allows the study of the three-dimensional structure of proteins and other macromolecular assemblies of biological interest. Its primary data consists of transmission electron microscopy images from multiple copies of the molecule in random orientations. Such images are very noisy due to the low electron dose employed. Reconstruction of the macromolecule can be obtained by averaging many images of particles in similar orientations and estimating their relative angles. However, heterogeneous conformational states often co-exist in the sample, because the molecular complexes can be flexible and may also interact with other particles. Heterogeneity poses a challenge to the reconstruction of reliable 3D models and degrades their resolution. Among the most popular algorithms used for structural classification are k-means clustering, hierarchical clustering, self-organizing maps and maximum-likelihood estimators. Such approaches are usually interlaced with the reconstructions of the 3D models. Nevertheless, recent works indicate that it is possible to infer information about the structure of the molecules directly from the dataset of 2D projections. Among these findings is the relationship between structural variability and manifolds in a multidimensional feature space. This dissertation investigates whether an ensemble of unsupervised classification algorithms is able to separate these "conformational manifolds". Ensemble or "consensus" methods tend to provide more accurate classification and may achieve satisfactory performance across a wide range of datasets, when compared with individual algorithms. We investigate the behavior of six clustering algorithms both individually and combined in ensembles for the task of structural heterogeneity classification. The approach was tested on synthetic and real datasets containing a mixture of images from the Mm-cpn chaperonin in the "open" and "closed" states. It is shown that cluster ensembles can provide useful information in validating the structural partitionings independently of 3D reconstruction methodsMestradoEngenharia de ComputaçãoMestre em Engenharia Elétric

Detecting Sunyaev-Zel'dovich clusters with PLANCK: III. Properties of the expected SZ-cluster sample

The PLANCK-mission is the most sensitive all-sky submillimetric mission currently being planned and prepared. Special emphasis is given to the observation of clusters of galaxies by their thermal Sunyaev-Zel'dovich (SZ) effect. In this work, the results of a simulation are presented that combines all-sky maps of the thermal and kinetic SZ-effect with cosmic microwave background (CMB) fluctuations, Galactic foregrounds (synchrotron emission, thermal emission from dust, free-free emission and rotational transitions of carbon monoxide molecules) and sub-millimetric emission from planets and asteroids of the Solar System. Observational issues, such as PLANCKs beam shapes, frequency response and spatially non-uniform instrumental noise have been incorporated. Matched and scale-adaptive multi-frequency filtering schemes have been extended to spherical coordinates and are now applied to the data sets in order to isolate and amplify the weak thermal SZ-signal. The properties of the resulting SZ-cluster sample are characterised in detail: Apart from the number of clusters as a function of cluster parameters such as redshift z and total mass M, the distribution n(sigma)d sigma of the detection significance sigma, the number of detectable clusters in relation to the model cluster parameters entering the filter construction, the position accuracy of an SZ-detection and the cluster number density as a function of ecliptic latitude beta is examined.Comment: 14 pages, 16 figures, 13 tables, submitted to MNRAS, 16.Feb.200