Development of image mapping spectrometer (IMS) for hyperspectral fluorescence microscopy

An image mapping spectrometer (IMS) for microscopy applications is presented. Its principle is based on the redirecting of image zones by specially organized thin mirrors within a custom fabricated component termed an image mapper. The demonstrated prototype can simultaneously acquire a 140nm spectral range within its 2D field of view from a single image. The spectral resolution of the system is 5.6nm. The FOV and spatial resolution of the IMS depend on the selected microscope objective and for the results presented is 45x45mu 2 and 0.45mum respectively. The system requires no scanning and minimal post data processing. In addition, the reflective nature of the image mapper and use of prisms for spectral dispersion make the system light efficient. Both of the above features are highly valuable for real time fluorescent-spectral imaging in biological and diagnostic applications

Snapshot spectrally encoded fluorescence imaging through a fiber bundle

Fiber optic endomicroscopy is a valuable tool for clinical diagnostics and animal studies because it can capture images of tissue in vivo with subcellular resolution. Current configurations for endomicroscopes have either limited spatial resolution or require a scanning mechanism at the distal end of the fiber, which can slow imaging speed and increase the probe size. We present a novel configuration that provides high contrast 350 × 350 pixel images at 7.2 frames per second, without the need for mechanical scanning at the proximal or distal end of the fiber. The proofof- concept benchtop system is tested in fluorescence mode and can resolve 1.5 μm features of a high resolution 1951 USAF target

Remote identification of overhead conductor materials

The recent draft determination by Australian Energy Regulator has meant a probable large reduction of approximately 30% in the income able to be generated by Ergon Energy. This recent ruling has increased the focus on cost effective and timely solutions to problems and encouraged continual evaluation of emerging technology which may facilitate these solutions. This focus has become a primary consideration in the organisation. The five main types of conductor present in the Ergon Energy bare overhead network in copper, galvanised steel, steel re-enforced aluminium, aluminium and aluminium alloy, which all age in a variety of ways when exposed to the elements. With over 1,800 circuit kilometres of unidentified conductor in the Ergon Energy network and a further unknown amount of incorrectly identified conductor, suitably managing the risk of conductor failure in a targeted, efficient, and timely manner is problematic. Spectral analysis is rapidly maturing as a technology with recent uses including satellite imagery to identify mineral deposits, analysis of distant planets, and also includes uses such as identifying cancerous growths in the human body, identifying scrap metals, evaluating the contamination of land by various contaminants, and helping naval vessels avoid mines by identification of these metal objects in the ocean. Initial measurements in the visible spectrum were taken with a low cost commercial USB plug and play spectrometer which identified significant differences in the spectral responses of copper, steel and aluminium. This spectrometer gave relatively constant results for aluminium, and galvanised steel under various lighting conditions, sample ages, and sample sizes. Consistent results were also obtained of various copper sample sizes and lighting conditions, however the variable surface patina’s due to weathering resulted in inconsistent results. The spectrometer could not discern between all aluminium conductor (AAC) and all aluminium alloy conductor (AAAC). An attempt was made to build an image spectrometer from a Nikon D700 consumer camera which was partially successful. The device was successful in recording relatively accurately the dominant wavelengths of a compact fluorescent light source and did record two measurements of aluminium conductor with moderate accuracy. Difficulties were encountered with aligning the optical path, and artefacts being introduced in the optical path