Simultaneous multiplane imaging with reverberation multiphoton microscopy

Multiphoton microscopy (MPM) has gained enormous popularity over the years for its capacity to provide high resolution images from deep within scattering samples1. However, MPM is generally based on single-point laser-focus scanning, which is intrinsically slow. While imaging speeds as fast as video rate have become routine for 2D planar imaging, such speeds have so far been unattainable for 3D volumetric imaging without severely compromising microscope performance. We demonstrate here 3D volumetric (multiplane) imaging at the same speed as 2D planar (single plane) imaging, with minimal compromise in performance. Specifically, multiple planes are acquired by near-instantaneous axial scanning while maintaining 3D micron-scale resolution. Our technique, called reverberation MPM, is well adapted for large-scale imaging in scattering media with low repetition-rate lasers, and can be implemented with conventional MPM as a simple add-on.Accepted manuscrip

Accelerated High-Resolution Photoacoustic Tomography via Compressed Sensing

Current 3D photoacoustic tomography (PAT) systems offer either high image quality or high frame rates but are not able to deliver high spatial and temporal resolution simultaneously, which limits their ability to image dynamic processes in living tissue. A particular example is the planar Fabry-Perot (FP) scanner, which yields high-resolution images but takes several minutes to sequentially map the photoacoustic field on the sensor plane, point-by-point. However, as the spatio-temporal complexity of many absorbing tissue structures is rather low, the data recorded in such a conventional, regularly sampled fashion is often highly redundant. We demonstrate that combining variational image reconstruction methods using spatial sparsity constraints with the development of novel PAT acquisition systems capable of sub-sampling the acoustic wave field can dramatically increase the acquisition speed while maintaining a good spatial resolution: First, we describe and model two general spatial sub-sampling schemes. Then, we discuss how to implement them using the FP scanner and demonstrate the potential of these novel compressed sensing PAT devices through simulated data from a realistic numerical phantom and through measured data from a dynamic experimental phantom as well as from in-vivo experiments. Our results show that images with good spatial resolution and contrast can be obtained from highly sub-sampled PAT data if variational image reconstruction methods that describe the tissues structures with suitable sparsity-constraints are used. In particular, we examine the use of total variation regularization enhanced by Bregman iterations. These novel reconstruction strategies offer new opportunities to dramatically increase the acquisition speed of PAT scanners that employ point-by-point sequential scanning as well as reducing the channel count of parallelized schemes that use detector arrays.Comment: submitted to "Physics in Medicine and Biology

Graphics processing unit accelerating compressed sensing photoacoustic computed tomography with total variation

Photoacoustic computed tomography with compressed sensing (CS-PACT) is a commonly used imaging strategy for sparse-sampling PACT. However, it is very time-consuming because of the iterative process involved in the image reconstruction. In this paper, we present a graphics processing unit (GPU)-based parallel computation framework for total-variation-based CS-PACT and adapted into a custom-made PACT system. Specifically, five compute-intensive operators are extracted from the iteration algorithm and are redesigned for parallel performance on a GPU. We achieved an image reconstruction speed 24–31 times faster than the CPU performance. We performed in vivo experiments on human hands to verify the feasibility of our developed method

Micromachined Scanning Devices for 3D Acoustic Imaging

Acoustic imaging (including ultrasound and photoacoustic imaging) refers to a class of imaging methods that use high-frequency sound (ultrasound) waves to generate contrast images for the interrogated media. It provides 3D spatial distribution of structural, mechanical, and even compositional properties in different materials. To conduct 3D ultrasound imaging, 2D ultrasound transducer arrays followed by multi-channel high-frequency data acquisition (DAQ) systems are frequently used. However, as the quantity and density of the transducer elements and also the DAQ channels increase, the acoustic imaging system becomes complex, bulky, expensive, and also power consuming. This situation is especially true for 3D imaging systems, where a 2D transducer array with hundreds or even thousands of elements could be involved. To address this issue, the objective of this research is to achieve new micromachined scanning devices to enable fast and versatile 2D ultrasound signal acquisition for 3D image reconstruction without involving complex physical transducer arrays and DAQ electronics. The new micromachined scanning devices studied in this research include 1) a water-immersible scanning mirror microsystem, 2) a micromechanical scanning transducer, and 3) a multi-layer linear transducer array. Especially, the water-immersible scanning mirror microsystem is capable of scanning focused ultrasound beam (from a single-element transducer) in two dimensions for 3D high-resolution acoustic microscopy. The micromechanical scanning transducer is capable of sending and receiving ultrasound signal from a single-element transducer to a 2D array of locations for 3D acoustic tomography. The multi-layer linear transducer array allows a unique electronic scanning scheme to simulate the functioning of a much larger 2D transducer array for 3D acoustic tomography. The design, fabrication and testing of the above three devices have been successfully accomplished and their applications in 3D acoustic microscopy and tomography have been demonstrated

Micromachined Scanning Devices for 3D Acoustic Imaging

Acoustic imaging (including ultrasound and photoacoustic imaging) refers to a class of imaging methods that use high-frequency sound (ultrasound) waves to generate contrast images for the interrogated media. It provides 3D spatial distribution of structural, mechanical, and even compositional properties in different materials. To conduct 3D ultrasound imaging, 2D ultrasound transducer arrays followed by multi-channel high-frequency data acquisition (DAQ) systems are frequently used. However, as the quantity and density of the transducer elements and also the DAQ channels increase, the acoustic imaging system becomes complex, bulky, expensive, and also power consuming. This situation is especially true for 3D imaging systems, where a 2D transducer array with hundreds or even thousands of elements could be involved. To address this issue, the objective of this research is to achieve new micromachined scanning devices to enable fast and versatile 2D ultrasound signal acquisition for 3D image reconstruction without involving complex physical transducer arrays and DAQ electronics. The new micromachined scanning devices studied in this research include 1) a water-immersible scanning mirror microsystem, 2) a micromechanical scanning transducer, and 3) a multi-layer linear transducer array. Especially, the water-immersible scanning mirror microsystem is capable of scanning focused ultrasound beam (from a single-element transducer) in two dimensions for 3D high-resolution acoustic microscopy. The micromechanical scanning transducer is capable of sending and receiving ultrasound signal from a single-element transducer to a 2D array of locations for 3D acoustic tomography. The multi-layer linear transducer array allows a unique electronic scanning scheme to simulate the functioning of a much larger 2D transducer array for 3D acoustic tomography. The design, fabrication and testing of the above three devices have been successfully accomplished and their applications in 3D acoustic microscopy and tomography have been demonstrated

Scanning ultrafast electron microscopy

Progress has been made in the development of four-dimensional ultrafast electron microscopy, which enables space-time imaging of structural dynamics in the condensed phase. In ultrafast electron microscopy, the electrons are accelerated, typically to 200 keV, and the microscope operates in the transmission mode. Here, we report the development of scanning ultrafast electron microscopy using a field-emission-source configuration. Scanning of pulses is made in the single-electron mode, for which the pulse contains at most one or a few electrons, thus achieving imaging without the space-charge effect between electrons, and still in ten(s) of seconds. For imaging, the secondary electrons from surface structures are detected, as demonstrated here for material surfaces and biological specimens. By recording backscattered electrons, diffraction patterns from single crystals were also obtained. Scanning pulsed-electron microscopy with the acquired spatiotemporal resolutions, and its efficient heat-dissipation feature, is now poised to provide in situ 4D imaging and with environmental capability

Advances in test and measurement of the interface adhesion and bond strengths in coating-substrate systems, emphasising blister and bulk techniques

In this paper, recent advances in the minimum-destructive testing of the adhesion of coating-substrate systems are reviewed, focusing on key techniques such as micro- and nano-scale levels of indentation, scratching, laser-induced wave shock, as well as the blister and buckle approach. Along with adhesion failure tests, the latest and most extensive applications of the adhesion test methods in nano-, micro- and bulk-coating technology and the associated techniques to determine the minimum damage defects left on the coatings are discussed and their use reviewed

Fast scanning two-photon microscope

Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2010.Cataloged from PDF version of thesis.Includes bibliographical references (p. 45-46).Fast scanning two-photon microscopy coupled with the use light activated ion channels provides the basis for fast imaging and stimulation in the characterization of in vivo neural networks. A two-photon microscope capable of fast scanning using acousto-optic deflectors was designed and implemented. The software controller was expanded so that random access scan in three dimensions could be handled, so that algorithms that can identify neurons from images acquired using the two-photon microscope can be developed. Finally the localization of optogenetic Channelrhodopsin-2 channel to the neuron cell body was tested using a ChR2-MBD construct.by Jeremy T Chang.M.Eng