31,711 research outputs found
Automatic assignment of protein backbone resonances by direct spectrum inspection in targeted acquisition of NMR data
The necessity to acquire large multidimensional datasets, a basis for assignment of NMR resonances, results in long data acquisition times during which substantial degradation of a protein sample might occur. Here we propose a method applicable for such a protein for automatic assignment of backbone resonances by direct inspection of multidimensional NMR spectra. In order to establish an optimal balance between completeness of resonance assignment and losses of cross-peaks due to dynamic processes/degradation of protein, assignment of backbone resonances is set as a stirring criterion for dynamically controlled targeted nonlinear NMR data acquisition. The result is demonstrated with the 12kDa 13C,15N-labeled apo-form of heme chaperone protein CcmE, where hydrolytic cleavage of 29 C-terminal amino acids is detected. For this protein, 90 and 98% of manually assignable resonances are automatically assigned within 10 and 40h of nonlinear sampling of five 3D NMR spectra, respectively, instead of 600h needed to complete the full time domain grid. In addition, resonances stemming from degradation products are identified. This study indicates that automatic resonance assignment might serve as a guiding criterion for optimal run-time allocation of NMR resources in applications to proteins prone to degradatio
Improving and Assessing Planet Sensitivity of the GPI Exoplanet Survey with a Forward Model Matched Filter
We present a new matched filter algorithm for direct detection of point
sources in the immediate vicinity of bright stars. The stellar Point Spread
Function (PSF) is first subtracted using a Karhunen-Lo\'eve Image Processing
(KLIP) algorithm with Angular and Spectral Differential Imaging (ADI and SDI).
The KLIP-induced distortion of the astrophysical signal is included in the
matched filter template by computing a forward model of the PSF at every
position in the image. To optimize the performance of the algorithm, we conduct
extensive planet injection and recovery tests and tune the exoplanet spectra
template and KLIP reduction aggressiveness to maximize the Signal-to-Noise
Ratio (SNR) of the recovered planets. We show that only two spectral templates
are necessary to recover any young Jovian exoplanets with minimal SNR loss. We
also developed a complete pipeline for the automated detection of point source
candidates, the calculation of Receiver Operating Characteristics (ROC), false
positives based contrast curves, and completeness contours. We process in a
uniform manner more than 330 datasets from the Gemini Planet Imager Exoplanet
Survey (GPIES) and assess GPI typical sensitivity as a function of the star and
the hypothetical companion spectral type. This work allows for the first time a
comparison of different detection algorithms at a survey scale accounting for
both planet completeness and false positive rate. We show that the new forward
model matched filter allows the detection of fainter objects than a
conventional cross-correlation technique with a Gaussian PSF template for the
same false positive rate.Comment: ApJ accepte
The stellar content of the Hamburg/ESO survey. IV. Selection of candidate metal-poor stars
We present the quantitative methods used for selecting candidate metal-poor
stars in the Hamburg/ESO objective-prism survey (HES). The selection is based
on the strength of the Ca II K line, B-V colors (both measured directly from
the digital HES spectra), as well as J-K colors from the 2 Micron All Sky
Survey. The KP index for Ca II K can be measured from the HES spectra with an
accuracy of 1.0 Angstrom, and a calibration of the HES B-V colors, using CCD
photometry, yields a 1-sigma uncertainty of 0.07 mag for stars in the color
range 0.3 < B-V < 1.4. These accuracies make it possible to reliably reject
stars with [Fe/H] > -2.0 without sacrificing completeness at the lowest
metallicities. A test of the selection using 1121 stars of the HK survey of
Beers, Preston, and Shectman present on HES plates suggests that the
completeness at [Fe/H] < -3.5 is close to 100% and that, at the same time, the
contamination of the candidate sample with false positives is low: 50% of all
stars with [Fe/H] > -2.5 and 97% of all stars with [Fe/H] > -2.0 are rejected.
The selection was applied to 379 HES fields, covering a nominal area of 8853
square degrees of the southern high Galactic latitude sky. The candidate sample
consists of 20,271 stars in the magnitude range 10 < B < 18. A comparison of
the magnitude distribution with that of the HK survey shows that the magnitude
limit of the HES sample is about 2 mag fainter. Taking the overlap of the sky
areas covered by both surveys into account, it follows that the survey volume
for metal-poor stars has been increased by the HES by about a factor of 10 with
respect to the HK survey. We have already identified several very rare objects
with the HES, including, e.g., the three most heavy-element deficient stars
currently known.Comment: 11 pages, 10 figures, accepted for publication in A&
Identifying Ultra-Cool Dwarfs at Low Galactic Latitudes: A Southern Candidate Catalogue
We present an Ultra-Cool Dwarf (UCD) catalogue compiled from low southern
Galactic latitudes and mid-plane, from a cross-correlation of the 2MASS and
SuperCOSMOS surveys. The catalogue contains 246 members identified from 5042
sq. deg. within 220 deg. <= l <= 360 deg. and 0 deg. < l <= 30 deg., for |b| <=
15 deg. Sixteen candidates are spectroscopically confirmed in the near-IR as
UCDs with spectral types from M7.5V to L9. Our catalogue selection method is
presented enabling UCDs from ~M8V to the L-T transition to be selected down to
a 2MASS limiting magnitude of Ks ~= 14.5 mag. This method does not require
candidates to have optical detections for catalogue inclusion. An optimal set
of optical/near-IR and reduced proper-motion selection criteria have been
defined that includes: an Rf and Ivn photometric surface gravity test, a dual
Rf-band variability check, and an additional photometric classification scheme
to selectively limit contaminants. We identify four candidates as possible
companions to nearby Hipparcos stars -- observations are needed to identify
these as potential benchmark UCD companions. We also identify twelve UCDs
within a possible distance 20 pc, three are previously unknown of which two are
estimated within 10 pc, complimenting the nearby volume-limited census of UCDs.
An analysis of the catalogue spatial completeness provides estimates for
distance completeness over three UCD MJ ranges, while Monte-Carlo simulations
provide an estimate of catalogue areal completeness at the 75 per cent level.
We estimate a UCD space density of Rho (total) = (6.41+-3.01)x10^3/pc^3 over
the range of 10.5 <= MJ ~< 14.9, similar to values measured at higher Galactic
latitudes (|b| ~> 10 deg.) in the field population and obtained from more
robust spectroscopically confirmed UCD samples.Comment: MNRAS accepted April 2012. Contains 30 figures and 11 tables. Tables
2 and 6 to be published in full and on-line only. The on-line tables can also
be obtained by contacting the author
A QSO survey via optical variability and zero proper motion in the M92 field. IV. More QSOs due to improved photometry
We continue the QSO search in the 10 square degrees Schmidt field around M92
based on variability and proper motion (VPM) constraints. We have re-reduced
162 digitised B plates with a time-baseline of more than three decades and have
considerably improved both the photometric accuracy and the star-galaxy
separation at B>19. QSO candidates are selected and marked with one out of
three degrees of priority based on the statistical significance of their
measured variability and zero proper motion. Spectroscopic follow-up
observations of 84 new candidates with B>19 revealed an additional 37 QSOs and
7 Seyfert1s. In particular, all 92 high-priority candidates are
spectroscopically classified now; among them are 70 QSOs and 9 Seyfert1s
(success rate 86%). We expect that 87% (55%) of all QSOs with B<19.0 (19.8) are
contained in this high-priority subsample. For the combined sample of
high-priority and medium-priority objects, a completeness of 89% is estimated
up to B_lim=19.5. The sample of all AGNs detected in the framework of the VPM
search in the M92 field contains now 95 QSOs and 14 Seyfert1s with B<19.9.
Although the VPM QSOs were selected by completely different criteria, their
properties do not significantly differ from those of QSOs found by more
traditional optical survey techniques. In particular, the spectra and the
optical broad band colours do not provide any hints on a substantial population
of red QSOs up to the present survey limit.Comment: 13 pages, 12 figures, accepted for publication in Astronomy and
Astrophysic
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